Phylogenetic and transcripts profiling of glucose sensing related genes in Candida glabrata
Background: The sensing mechanism of glucose in Saccharomyces cerevisiae is well studied. However, such information is scarcely found in other yeast species such as Candida glabrata. Objectives: This study aimed to identify the glucose sensing pathway related genes of C. glabrata and to analyze the...
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| 格式: | 文件 |
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Ahvaz Jundishapur University of Medical Sciences
2015
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| _version_ | 1825948541818765312 |
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| author | Ng, Tzu Shan Mohd Desa, Mohd Nasir Sandai, Doblin Chong, Pei Pei Than , Leslie Thian Lung |
| author_facet | Ng, Tzu Shan Mohd Desa, Mohd Nasir Sandai, Doblin Chong, Pei Pei Than , Leslie Thian Lung |
| author_sort | Ng, Tzu Shan |
| collection | UPM |
| description | Background: The sensing mechanism of glucose in Saccharomyces cerevisiae is well studied. However, such information is scarcely found in other yeast species such as Candida glabrata.
Objectives: This study aimed to identify the glucose sensing pathway related genes of C. glabrata and to analyze the regulation pattern of these genes in response to different surrounding glucose concentrations through the quantitative real time polymerase chain reaction (qRT-PCR).
Materials and Methods: Phylogenetic analysis was carried out on predicted amino acid sequences of C. glabrata and S. cerevisiae to compare their degree of similarity. In addition, the growth of C. glabrata in response to different amounts of glucose (0%, 0.01%, 0.1%, 1% and 2%) was evaluated via the spot dilution assay on prepared agar medium. Besides, the SNF3 and RGT2, which act as putative glucose sensors, and the RGT1 and MIG1, which act as putative transcriptional regulators and selected downstream hexose transporters (HXTs), were analysed through qRT-PCR analysis for the gene expression level under different glucose concentrations.
Results: Comparative analysis of predicted amino acids in the phylogenetic tree showed high similarity between C. glabrata and S cerevisiae. Besides, C. glabrata demonstrated the capability to grow in glucose levels as low as 0.01% in the spot dilution assay. In qRT-PCR analysis, differential expressions were observed in selected genes when C. glabrata was subjected to different glucose concentrations.
Conclusions: The constructed phylogenetic tree suggests the close evolutionary relationship between C. glabrata and S. cerevisiae. The capability of C. glabrata to grow in extremely low glucose environments and the differential expression of selected glucose-sensing related genes suggested the possible role of these genes in modulating the growth of C. glabrata in response to different glucose concentrations. This study helps deepen our understanding of the glucose sensing mechanism in C. glabrata and serves to provide fundamental data that may assist in unveiling this mechanism as a potential drug target.
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| first_indexed | 2024-03-06T08:30:27Z |
| format | Article |
| id | upm.eprints-34908 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:30:27Z |
| publishDate | 2015 |
| publisher | Ahvaz Jundishapur University of Medical Sciences |
| record_format | dspace |
| spelling | upm.eprints-349082015-12-23T11:11:08Z http://psasir.upm.edu.my/id/eprint/34908/ Phylogenetic and transcripts profiling of glucose sensing related genes in Candida glabrata Ng, Tzu Shan Mohd Desa, Mohd Nasir Sandai, Doblin Chong, Pei Pei Than , Leslie Thian Lung Background: The sensing mechanism of glucose in Saccharomyces cerevisiae is well studied. However, such information is scarcely found in other yeast species such as Candida glabrata. Objectives: This study aimed to identify the glucose sensing pathway related genes of C. glabrata and to analyze the regulation pattern of these genes in response to different surrounding glucose concentrations through the quantitative real time polymerase chain reaction (qRT-PCR). Materials and Methods: Phylogenetic analysis was carried out on predicted amino acid sequences of C. glabrata and S. cerevisiae to compare their degree of similarity. In addition, the growth of C. glabrata in response to different amounts of glucose (0%, 0.01%, 0.1%, 1% and 2%) was evaluated via the spot dilution assay on prepared agar medium. Besides, the SNF3 and RGT2, which act as putative glucose sensors, and the RGT1 and MIG1, which act as putative transcriptional regulators and selected downstream hexose transporters (HXTs), were analysed through qRT-PCR analysis for the gene expression level under different glucose concentrations. Results: Comparative analysis of predicted amino acids in the phylogenetic tree showed high similarity between C. glabrata and S cerevisiae. Besides, C. glabrata demonstrated the capability to grow in glucose levels as low as 0.01% in the spot dilution assay. In qRT-PCR analysis, differential expressions were observed in selected genes when C. glabrata was subjected to different glucose concentrations. Conclusions: The constructed phylogenetic tree suggests the close evolutionary relationship between C. glabrata and S. cerevisiae. The capability of C. glabrata to grow in extremely low glucose environments and the differential expression of selected glucose-sensing related genes suggested the possible role of these genes in modulating the growth of C. glabrata in response to different glucose concentrations. This study helps deepen our understanding of the glucose sensing mechanism in C. glabrata and serves to provide fundamental data that may assist in unveiling this mechanism as a potential drug target. Ahvaz Jundishapur University of Medical Sciences 2015-11 Article PeerReviewed Ng, Tzu Shan and Mohd Desa, Mohd Nasir and Sandai, Doblin and Chong, Pei Pei and Than , Leslie Thian Lung (2015) Phylogenetic and transcripts profiling of glucose sensing related genes in Candida glabrata. Jundishapur Journal of Microbiology, 8 (11). art. no. e25177. pp. 1-9. ISSN 2008-3645; ESSN: 2008-4161 http://jjmicrobiol.com/?page=article&article_id=25177 10.5812/jjm.25177 |
| spellingShingle | Ng, Tzu Shan Mohd Desa, Mohd Nasir Sandai, Doblin Chong, Pei Pei Than , Leslie Thian Lung Phylogenetic and transcripts profiling of glucose sensing related genes in Candida glabrata |
| title | Phylogenetic and transcripts profiling of glucose sensing related genes in Candida glabrata |
| title_full | Phylogenetic and transcripts profiling of glucose sensing related genes in Candida glabrata |
| title_fullStr | Phylogenetic and transcripts profiling of glucose sensing related genes in Candida glabrata |
| title_full_unstemmed | Phylogenetic and transcripts profiling of glucose sensing related genes in Candida glabrata |
| title_short | Phylogenetic and transcripts profiling of glucose sensing related genes in Candida glabrata |
| title_sort | phylogenetic and transcripts profiling of glucose sensing related genes in candida glabrata |
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