Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells
Lactobacillus acidophilus is categorized as a probiotic strain because of its beneficial effects in human health and prevention of disease transmission. This study is aimed to characterize the probiotic potential of L. acidophilus 36YL originally isolated from the vagina of healthy and fertile Irani...
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| Format: | Article |
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Elsevier
2014
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| _version_ | 1825948572698279936 |
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| author | Nami, Yousef Abdullah, Norhafizah Haghshenas, Babak Awang Biak, Dayang Radiah Rosli, Rozita Khosroushahi, Ahmad Yari |
| author_facet | Nami, Yousef Abdullah, Norhafizah Haghshenas, Babak Awang Biak, Dayang Radiah Rosli, Rozita Khosroushahi, Ahmad Yari |
| author_sort | Nami, Yousef |
| collection | UPM |
| description | Lactobacillus acidophilus is categorized as a probiotic strain because of its beneficial effects in human health and prevention of disease transmission. This study is aimed to characterize the probiotic potential of L. acidophilus 36YL originally isolated from the vagina of healthy and fertile Iranian women. The L. acidophilus 36YL strain was identified using 16S rDNA gene sequencing and characterized by biochemical methodologies, such as antibiotics susceptibility, antimicrobial activity, and acid and bile resistance. The bioactivity of the secretion of this strain on four human cancer cell lines (AGS, HeLa, MCF-7, and HT-29) and one normal cell line (HUVEC) was evaluated by cytotoxicity assay and apoptosis analysis. This newly isolated strain was found to exhibit notable probiotic properties, such as admirable antibiotic susceptibility, good antimicrobial activity, and favorable resistance to acid and bile salt. The results of bioactivity assessment demonstrated acceptable anticancer effects on the four tested cancer cell lines and negligible side effects on the assayed normal cell line. Our findings revealed that the anticancer effect of L. acidophilus 36YL strain secretions depends on the induction of apoptosis in cancer cells. L. acidophilus 36YL strain is considered as a nutraceutical alternative or a topical medication with a potential therapeutic index because of the absence of cytotoxicity to normal cells, but effective toxicity to cancer cell lines. |
| first_indexed | 2024-03-06T08:31:10Z |
| format | Article |
| id | upm.eprints-35065 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:31:10Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-350652015-12-29T07:51:51Z http://psasir.upm.edu.my/id/eprint/35065/ Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells Nami, Yousef Abdullah, Norhafizah Haghshenas, Babak Awang Biak, Dayang Radiah Rosli, Rozita Khosroushahi, Ahmad Yari Lactobacillus acidophilus is categorized as a probiotic strain because of its beneficial effects in human health and prevention of disease transmission. This study is aimed to characterize the probiotic potential of L. acidophilus 36YL originally isolated from the vagina of healthy and fertile Iranian women. The L. acidophilus 36YL strain was identified using 16S rDNA gene sequencing and characterized by biochemical methodologies, such as antibiotics susceptibility, antimicrobial activity, and acid and bile resistance. The bioactivity of the secretion of this strain on four human cancer cell lines (AGS, HeLa, MCF-7, and HT-29) and one normal cell line (HUVEC) was evaluated by cytotoxicity assay and apoptosis analysis. This newly isolated strain was found to exhibit notable probiotic properties, such as admirable antibiotic susceptibility, good antimicrobial activity, and favorable resistance to acid and bile salt. The results of bioactivity assessment demonstrated acceptable anticancer effects on the four tested cancer cell lines and negligible side effects on the assayed normal cell line. Our findings revealed that the anticancer effect of L. acidophilus 36YL strain secretions depends on the induction of apoptosis in cancer cells. L. acidophilus 36YL strain is considered as a nutraceutical alternative or a topical medication with a potential therapeutic index because of the absence of cytotoxicity to normal cells, but effective toxicity to cancer cell lines. Elsevier 2014-08 Article PeerReviewed Nami, Yousef and Abdullah, Norhafizah and Haghshenas, Babak and Awang Biak, Dayang Radiah and Rosli, Rozita and Khosroushahi, Ahmad Yari (2014) Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells. Anaerobe, 28. pp. 29-36. ISSN 1075-9964; ESSN: 1095-8274 http://www.sciencedirect.com/science/article/pii/S107599641400050X 10.1016/j.anaerobe.2014.04.012 |
| spellingShingle | Nami, Yousef Abdullah, Norhafizah Haghshenas, Babak Awang Biak, Dayang Radiah Rosli, Rozita Khosroushahi, Ahmad Yari Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells |
| title | Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells |
| title_full | Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells |
| title_fullStr | Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells |
| title_full_unstemmed | Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells |
| title_short | Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells |
| title_sort | probiotic potential and biotherapeutic effects of newly isolated vaginal lactobacillus acidophilus 36yl strain on cancer cells |
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