Protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil
Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Trans Tech Publications
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35094/1/Protein%20produced%20by%20Bacillus%20subtilis%20ATCC21332%20in%20the%20presence%20of%20Cymbopogon%20flexuosus%20essential%20oil.pdf |
| _version_ | 1796972637883203584 |
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| author | Mohd Noor, Hanina Muhamad, Hairul Shahril Ismail, Ismatul Nurul Asyikin Mat Radzi, Salina Mohamed Rehan, Maryam Abdul Kader, Abdul Jalil Mohamad, Rosfarizan |
| author_facet | Mohd Noor, Hanina Muhamad, Hairul Shahril Ismail, Ismatul Nurul Asyikin Mat Radzi, Salina Mohamed Rehan, Maryam Abdul Kader, Abdul Jalil Mohamad, Rosfarizan |
| author_sort | Mohd Noor, Hanina |
| collection | UPM |
| description | Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oil from Cymbopogon flexuosus in regulating proteins production by Bacillus subtilis ATCC21332. The Minimum Inhibition Concentration (MIC) of the C. flexuosus essential oil on B. subtilis was determined by using microdilution assay, resulting 1.76mg/ml. The bacteria cells were further exposed to the C. flexuosus essential oil at concentration of 0.01 MIC for 72 h. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins profile showed that a band with approximate size of 30 kDa was appeared for the treated bacteria with C. flexuosus essential oil. Thus, B. subtilis ATCC21332 in stressful condition with the presence of C. flexuosus essential oils at low concentration could induce the protein production. The isolated protein also showed antimicrobial activity against selected Gram-positive and Gram-negative bacteria. |
| first_indexed | 2024-03-06T08:31:15Z |
| format | Article |
| id | upm.eprints-35094 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:31:15Z |
| publishDate | 2014 |
| publisher | Trans Tech Publications |
| record_format | dspace |
| spelling | upm.eprints-350942016-10-11T02:22:57Z http://psasir.upm.edu.my/id/eprint/35094/ Protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil Mohd Noor, Hanina Muhamad, Hairul Shahril Ismail, Ismatul Nurul Asyikin Mat Radzi, Salina Mohamed Rehan, Maryam Abdul Kader, Abdul Jalil Mohamad, Rosfarizan Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oil from Cymbopogon flexuosus in regulating proteins production by Bacillus subtilis ATCC21332. The Minimum Inhibition Concentration (MIC) of the C. flexuosus essential oil on B. subtilis was determined by using microdilution assay, resulting 1.76mg/ml. The bacteria cells were further exposed to the C. flexuosus essential oil at concentration of 0.01 MIC for 72 h. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins profile showed that a band with approximate size of 30 kDa was appeared for the treated bacteria with C. flexuosus essential oil. Thus, B. subtilis ATCC21332 in stressful condition with the presence of C. flexuosus essential oils at low concentration could induce the protein production. The isolated protein also showed antimicrobial activity against selected Gram-positive and Gram-negative bacteria. Trans Tech Publications 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35094/1/Protein%20produced%20by%20Bacillus%20subtilis%20ATCC21332%20in%20the%20presence%20of%20Cymbopogon%20flexuosus%20essential%20oil.pdf Mohd Noor, Hanina and Muhamad, Hairul Shahril and Ismail, Ismatul Nurul Asyikin and Mat Radzi, Salina and Mohamed Rehan, Maryam and Abdul Kader, Abdul Jalil and Mohamad, Rosfarizan (2014) Protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil. Key Engineering Materials, 594-595. pp. 370-377. ISSN 1013-9826; ESSN: 1662-9795 http://www.scientific.net/KEM.594-595.370 10.4028/www.scientific.net/KEM.594-595.370 |
| spellingShingle | Mohd Noor, Hanina Muhamad, Hairul Shahril Ismail, Ismatul Nurul Asyikin Mat Radzi, Salina Mohamed Rehan, Maryam Abdul Kader, Abdul Jalil Mohamad, Rosfarizan Protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil |
| title | Protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil |
| title_full | Protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil |
| title_fullStr | Protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil |
| title_full_unstemmed | Protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil |
| title_short | Protein produced by Bacillus subtilis ATCC21332 in the presence of Cymbopogon flexuosus essential oil |
| title_sort | protein produced by bacillus subtilis atcc21332 in the presence of cymbopogon flexuosus essential oil |
| url | http://psasir.upm.edu.my/id/eprint/35094/1/Protein%20produced%20by%20Bacillus%20subtilis%20ATCC21332%20in%20the%20presence%20of%20Cymbopogon%20flexuosus%20essential%20oil.pdf |
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