Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b
The periplasmic proteome of recombinant E. coli cells expressing human interferon-α2b (INF-α2b) was analysed by 2D-gel electrophoresis to find the most altered proteins. Of some unique up- and down-regulated proteins in the proteome, ten were identified by MS. The majority of the proteins belonged t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35096/1/Proteome%20analysis%20of%20Escherichia%20coli%20periplasmic%20proteins%20in%20response%20to%20over-expression%20of%20recombinant%20human%20interferon%20%CE%B12b.pdf |
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| author | Heidary, Somayyeh Abdul Rahim, Raha Eissazadeh, Samira Moeini, Hassan Leow, Adam Thean Chor Abdullah, Mohd Puad |
| author_facet | Heidary, Somayyeh Abdul Rahim, Raha Eissazadeh, Samira Moeini, Hassan Leow, Adam Thean Chor Abdullah, Mohd Puad |
| author_sort | Heidary, Somayyeh |
| collection | UPM |
| description | The periplasmic proteome of recombinant E. coli cells expressing human interferon-α2b (INF-α2b) was analysed by 2D-gel electrophoresis to find the most altered proteins. Of some unique up- and down-regulated proteins in the proteome, ten were identified by MS. The majority of the proteins belonged to the ABC transporter protein family. Other affected proteins were ones involved in the regulation of transcription such as DNA-binding response regulator, stress-related proteins and ecotin. Thus, the production of INF-α2b acts as a stress on the cells and results in the induction of various transporters and stress related proteins. |
| first_indexed | 2024-03-06T08:31:16Z |
| format | Article |
| id | upm.eprints-35096 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:31:16Z |
| publishDate | 2014 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-350962016-10-11T02:22:59Z http://psasir.upm.edu.my/id/eprint/35096/ Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b Heidary, Somayyeh Abdul Rahim, Raha Eissazadeh, Samira Moeini, Hassan Leow, Adam Thean Chor Abdullah, Mohd Puad The periplasmic proteome of recombinant E. coli cells expressing human interferon-α2b (INF-α2b) was analysed by 2D-gel electrophoresis to find the most altered proteins. Of some unique up- and down-regulated proteins in the proteome, ten were identified by MS. The majority of the proteins belonged to the ABC transporter protein family. Other affected proteins were ones involved in the regulation of transcription such as DNA-binding response regulator, stress-related proteins and ecotin. Thus, the production of INF-α2b acts as a stress on the cells and results in the induction of various transporters and stress related proteins. Springer 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35096/1/Proteome%20analysis%20of%20Escherichia%20coli%20periplasmic%20proteins%20in%20response%20to%20over-expression%20of%20recombinant%20human%20interferon%20%CE%B12b.pdf Heidary, Somayyeh and Abdul Rahim, Raha and Eissazadeh, Samira and Moeini, Hassan and Leow, Adam Thean Chor and Abdullah, Mohd Puad (2014) Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b. Biotechnology Letters, 36 (7). pp. 1479-1484. ISSN 0141-5492; ESSN: 1573-6776 http://link.springer.com/article/10.1007/s10529-014-1504-7?view=classic 10.1007/s10529-014-1504-7 |
| spellingShingle | Heidary, Somayyeh Abdul Rahim, Raha Eissazadeh, Samira Moeini, Hassan Leow, Adam Thean Chor Abdullah, Mohd Puad Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b |
| title | Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b |
| title_full | Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b |
| title_fullStr | Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b |
| title_full_unstemmed | Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b |
| title_short | Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b |
| title_sort | proteome analysis of escherichia coli periplasmic proteins in response to over expression of recombinant human interferon α2b |
| url | http://psasir.upm.edu.my/id/eprint/35096/1/Proteome%20analysis%20of%20Escherichia%20coli%20periplasmic%20proteins%20in%20response%20to%20over-expression%20of%20recombinant%20human%20interferon%20%CE%B12b.pdf |
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