Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b

The periplasmic proteome of recombinant E. coli cells expressing human interferon-α2b (INF-α2b) was analysed by 2D-gel electrophoresis to find the most altered proteins. Of some unique up- and down-regulated proteins in the proteome, ten were identified by MS. The majority of the proteins belonged t...

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Main Authors: Heidary, Somayyeh, Abdul Rahim, Raha, Eissazadeh, Samira, Moeini, Hassan, Leow, Adam Thean Chor, Abdullah, Mohd Puad
Format: Article
Language:English
Published: Springer 2014
Online Access:http://psasir.upm.edu.my/id/eprint/35096/1/Proteome%20analysis%20of%20Escherichia%20coli%20periplasmic%20proteins%20in%20response%20to%20over-expression%20of%20recombinant%20human%20interferon%20%CE%B12b.pdf
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author Heidary, Somayyeh
Abdul Rahim, Raha
Eissazadeh, Samira
Moeini, Hassan
Leow, Adam Thean Chor
Abdullah, Mohd Puad
author_facet Heidary, Somayyeh
Abdul Rahim, Raha
Eissazadeh, Samira
Moeini, Hassan
Leow, Adam Thean Chor
Abdullah, Mohd Puad
author_sort Heidary, Somayyeh
collection UPM
description The periplasmic proteome of recombinant E. coli cells expressing human interferon-α2b (INF-α2b) was analysed by 2D-gel electrophoresis to find the most altered proteins. Of some unique up- and down-regulated proteins in the proteome, ten were identified by MS. The majority of the proteins belonged to the ABC transporter protein family. Other affected proteins were ones involved in the regulation of transcription such as DNA-binding response regulator, stress-related proteins and ecotin. Thus, the production of INF-α2b acts as a stress on the cells and results in the induction of various transporters and stress related proteins.
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spelling upm.eprints-350962016-10-11T02:22:59Z http://psasir.upm.edu.my/id/eprint/35096/ Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b Heidary, Somayyeh Abdul Rahim, Raha Eissazadeh, Samira Moeini, Hassan Leow, Adam Thean Chor Abdullah, Mohd Puad The periplasmic proteome of recombinant E. coli cells expressing human interferon-α2b (INF-α2b) was analysed by 2D-gel electrophoresis to find the most altered proteins. Of some unique up- and down-regulated proteins in the proteome, ten were identified by MS. The majority of the proteins belonged to the ABC transporter protein family. Other affected proteins were ones involved in the regulation of transcription such as DNA-binding response regulator, stress-related proteins and ecotin. Thus, the production of INF-α2b acts as a stress on the cells and results in the induction of various transporters and stress related proteins. Springer 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35096/1/Proteome%20analysis%20of%20Escherichia%20coli%20periplasmic%20proteins%20in%20response%20to%20over-expression%20of%20recombinant%20human%20interferon%20%CE%B12b.pdf Heidary, Somayyeh and Abdul Rahim, Raha and Eissazadeh, Samira and Moeini, Hassan and Leow, Adam Thean Chor and Abdullah, Mohd Puad (2014) Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b. Biotechnology Letters, 36 (7). pp. 1479-1484. ISSN 0141-5492; ESSN: 1573-6776 http://link.springer.com/article/10.1007/s10529-014-1504-7?view=classic 10.1007/s10529-014-1504-7
spellingShingle Heidary, Somayyeh
Abdul Rahim, Raha
Eissazadeh, Samira
Moeini, Hassan
Leow, Adam Thean Chor
Abdullah, Mohd Puad
Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b
title Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b
title_full Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b
title_fullStr Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b
title_full_unstemmed Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b
title_short Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b
title_sort proteome analysis of escherichia coli periplasmic proteins in response to over expression of recombinant human interferon α2b
url http://psasir.upm.edu.my/id/eprint/35096/1/Proteome%20analysis%20of%20Escherichia%20coli%20periplasmic%20proteins%20in%20response%20to%20over-expression%20of%20recombinant%20human%20interferon%20%CE%B12b.pdf
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