Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and gamma-oryzanols in rice bran and rice bran oil
Rice bran and rice bran oil contain high amounts of lipid soluble phytochemicals such as tocopherols, tocotrienols and γ-oryzanols with potential health benefits. A reversed-phase high-performance liquid chromatographic method for the quantitation of these phytochemicals was developed. These phytoch...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer US
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35153/1/Rapid%20reversed.pdf |
| _version_ | 1796972650157834240 |
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| author | Shammugasamy, Balakrishnan Ramakrishnan, Yogeshini Manan, Farhiah Syed Muhammad, Sharifah Kharidah |
| author_facet | Shammugasamy, Balakrishnan Ramakrishnan, Yogeshini Manan, Farhiah Syed Muhammad, Sharifah Kharidah |
| author_sort | Shammugasamy, Balakrishnan |
| collection | UPM |
| description | Rice bran and rice bran oil contain high amounts of lipid soluble phytochemicals such as tocopherols, tocotrienols and γ-oryzanols with potential health benefits. A reversed-phase high-performance liquid chromatographic method for the quantitation of these phytochemicals was developed. These phytochemicals were extracted using rapid one-step methanol extraction and separated on a Kinetex pentafluorophenyl phase column for quantitation using fluorescence and ultraviolet detectors. Separation was carried out within 20 min at a flow rate of 1.0 mL/min using a gradient elution of mobile phase consisting of methanol and water at column temperature of 30 °C. A good separation of all eight tocol isomers was achieved while the γ-oryzanols were separated near to baseline. Extraction yields of tocopherols, tocotrienols and γ-oryzanols from saponification medium with different pH conditions were studied. Total γ-oryzanols extracted from alkaline medium were significantly lower than those from the neutral and acidic mediums (p < 0.05), while total tocols extracted from these mediums were not significantly different. The method gave a good calibration linear response (R² > 0.9994), low detection limit (3–34 ng/mL) and high precision (RSD < 7.5 %) with satisfactory recovery (R% = 81–116 %). The advantages of this method over available reverse-phase methods are the better resolution of β and γ tocol isomers with the γ-oryzanols, usage of non-halogenated mobile phase and shorter analysis time, and it is compatible with the standard laboratorial chromatographic equipment. |
| first_indexed | 2024-03-06T08:31:26Z |
| format | Article |
| id | upm.eprints-35153 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:31:26Z |
| publishDate | 2015 |
| publisher | Springer US |
| record_format | dspace |
| spelling | upm.eprints-351532016-01-22T02:42:22Z http://psasir.upm.edu.my/id/eprint/35153/ Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and gamma-oryzanols in rice bran and rice bran oil Shammugasamy, Balakrishnan Ramakrishnan, Yogeshini Manan, Farhiah Syed Muhammad, Sharifah Kharidah Rice bran and rice bran oil contain high amounts of lipid soluble phytochemicals such as tocopherols, tocotrienols and γ-oryzanols with potential health benefits. A reversed-phase high-performance liquid chromatographic method for the quantitation of these phytochemicals was developed. These phytochemicals were extracted using rapid one-step methanol extraction and separated on a Kinetex pentafluorophenyl phase column for quantitation using fluorescence and ultraviolet detectors. Separation was carried out within 20 min at a flow rate of 1.0 mL/min using a gradient elution of mobile phase consisting of methanol and water at column temperature of 30 °C. A good separation of all eight tocol isomers was achieved while the γ-oryzanols were separated near to baseline. Extraction yields of tocopherols, tocotrienols and γ-oryzanols from saponification medium with different pH conditions were studied. Total γ-oryzanols extracted from alkaline medium were significantly lower than those from the neutral and acidic mediums (p < 0.05), while total tocols extracted from these mediums were not significantly different. The method gave a good calibration linear response (R² > 0.9994), low detection limit (3–34 ng/mL) and high precision (RSD < 7.5 %) with satisfactory recovery (R% = 81–116 %). The advantages of this method over available reverse-phase methods are the better resolution of β and γ tocol isomers with the γ-oryzanols, usage of non-halogenated mobile phase and shorter analysis time, and it is compatible with the standard laboratorial chromatographic equipment. Springer US 2015-03 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35153/1/Rapid%20reversed.pdf Shammugasamy, Balakrishnan and Ramakrishnan, Yogeshini and Manan, Farhiah and Syed Muhammad, Sharifah Kharidah (2015) Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and gamma-oryzanols in rice bran and rice bran oil. Food Analytical Methods, 8 (3). 649-655 . ISSN 1936-9751; ESSN:1936-976X http://link.springer.com/article/10.1007%2Fs12161-014-9929-0 10.1007/s12161-014-9929-0 |
| spellingShingle | Shammugasamy, Balakrishnan Ramakrishnan, Yogeshini Manan, Farhiah Syed Muhammad, Sharifah Kharidah Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and gamma-oryzanols in rice bran and rice bran oil |
| title | Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and gamma-oryzanols in rice bran and rice bran oil |
| title_full | Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and gamma-oryzanols in rice bran and rice bran oil |
| title_fullStr | Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and gamma-oryzanols in rice bran and rice bran oil |
| title_full_unstemmed | Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and gamma-oryzanols in rice bran and rice bran oil |
| title_short | Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and gamma-oryzanols in rice bran and rice bran oil |
| title_sort | rapid reversed phase chromatographic method for determination of eight vitamin e isomers and gamma oryzanols in rice bran and rice bran oil |
| url | http://psasir.upm.edu.my/id/eprint/35153/1/Rapid%20reversed.pdf |
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