Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants
Background Regulatory T cells (Treg) play an essential role in early immune programming and shaping the immune response towards a pro-allergic or tolerant state. We evaluated cord blood Treg and cytokine responses to microbial and non-microbial stimuli in infants at high risk of allergic disease an...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Wiley-Blackwell Publishing Ltd.
2014
|
| _version_ | 1796972656106405888 |
|---|---|
| author | Ismail , Intan Hakimah Boyle, Robert J. Mah, Li Jeen Licciardi, Paul V. Tang, Mimi L. K. |
| author_facet | Ismail , Intan Hakimah Boyle, Robert J. Mah, Li Jeen Licciardi, Paul V. Tang, Mimi L. K. |
| author_sort | Ismail , Intan Hakimah |
| collection | UPM |
| description | Background
Regulatory T cells (Treg) play an essential role in early immune programming and shaping the immune response towards a pro-allergic or tolerant state. We evaluated cord blood Treg and cytokine responses to microbial and non-microbial stimuli in infants at high risk of allergic disease and their associations with development of allergic disease in the first year.
Methods
Cord blood mononuclear cells from 72 neonates were cultured with toll-like receptors (TLR2) ligands: lipoteichoic acid (LTA) and heat-killed Lactobacillus rhamnosus GG (HKL); TLR4 ligand: lipopolysaccharide (LPS); ovalbumin (OVA); anti-CD3; or media for 48 h. Treg numbers and Treg cytokines were assessed in relation to allergic disease outcomes during the first year of life (eczema and atopic sensitization).
Results
Infants with eczema (n = 24) had reduced percentages of FoxP3hiCD25hi Treg in LTA (p = 0.01, adj p = 0.005) and HKL (p = 0.04, adj p = 0.02) stimulated cultures as well as reduced IL-10 (p = 0.01) production following HKL stimulation compared to those without eczema (n = 48). No differences in Treg or cytokine responses to LPS, OVA or anti-CD3 were seen. Infants who developed sensitization had lower percentages of Treg following TLR2 stimulation (but not other stimuli) compared to non-sensitized infants.
Conclusions
High-risk children who develop allergic disease in the first year of life have deficient Treg responses to microbial stimuli but not allergen from the time of birth, which may contribute to failure of immune tolerance development in infancy. |
| first_indexed | 2024-03-06T08:31:34Z |
| format | Article |
| id | upm.eprints-35183 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:31:34Z |
| publishDate | 2014 |
| publisher | Wiley-Blackwell Publishing Ltd. |
| record_format | dspace |
| spelling | upm.eprints-351832015-12-31T02:11:48Z http://psasir.upm.edu.my/id/eprint/35183/ Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants Ismail , Intan Hakimah Boyle, Robert J. Mah, Li Jeen Licciardi, Paul V. Tang, Mimi L. K. Background Regulatory T cells (Treg) play an essential role in early immune programming and shaping the immune response towards a pro-allergic or tolerant state. We evaluated cord blood Treg and cytokine responses to microbial and non-microbial stimuli in infants at high risk of allergic disease and their associations with development of allergic disease in the first year. Methods Cord blood mononuclear cells from 72 neonates were cultured with toll-like receptors (TLR2) ligands: lipoteichoic acid (LTA) and heat-killed Lactobacillus rhamnosus GG (HKL); TLR4 ligand: lipopolysaccharide (LPS); ovalbumin (OVA); anti-CD3; or media for 48 h. Treg numbers and Treg cytokines were assessed in relation to allergic disease outcomes during the first year of life (eczema and atopic sensitization). Results Infants with eczema (n = 24) had reduced percentages of FoxP3hiCD25hi Treg in LTA (p = 0.01, adj p = 0.005) and HKL (p = 0.04, adj p = 0.02) stimulated cultures as well as reduced IL-10 (p = 0.01) production following HKL stimulation compared to those without eczema (n = 48). No differences in Treg or cytokine responses to LPS, OVA or anti-CD3 were seen. Infants who developed sensitization had lower percentages of Treg following TLR2 stimulation (but not other stimuli) compared to non-sensitized infants. Conclusions High-risk children who develop allergic disease in the first year of life have deficient Treg responses to microbial stimuli but not allergen from the time of birth, which may contribute to failure of immune tolerance development in infancy. Wiley-Blackwell Publishing Ltd. 2014-11 Article PeerReviewed Ismail , Intan Hakimah and Boyle, Robert J. and Mah, Li Jeen and Licciardi, Paul V. and Tang, Mimi L. K. (2014) Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants. Pediatric Allergy and Immunology, 25 (7). pp. 674-684. ISSN 0905-6157; ESSN: 1399-3038 http://onlinelibrary.wiley.com/doi/10.1111/pai.12303/abstract 10.1111/pai.12303 |
| spellingShingle | Ismail , Intan Hakimah Boyle, Robert J. Mah, Li Jeen Licciardi, Paul V. Tang, Mimi L. K. Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants |
| title | Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants |
| title_full | Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants |
| title_fullStr | Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants |
| title_full_unstemmed | Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants |
| title_short | Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants |
| title_sort | reduced neonatal regulatory t cell response to microbial stimuli associates with subsequent eczema in high risk infants |
| work_keys_str_mv | AT ismailintanhakimah reducedneonatalregulatorytcellresponsetomicrobialstimuliassociateswithsubsequenteczemainhighriskinfants AT boylerobertj reducedneonatalregulatorytcellresponsetomicrobialstimuliassociateswithsubsequenteczemainhighriskinfants AT mahlijeen reducedneonatalregulatorytcellresponsetomicrobialstimuliassociateswithsubsequenteczemainhighriskinfants AT licciardipaulv reducedneonatalregulatorytcellresponsetomicrobialstimuliassociateswithsubsequenteczemainhighriskinfants AT tangmimilk reducedneonatalregulatorytcellresponsetomicrobialstimuliassociateswithsubsequenteczemainhighriskinfants |