Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): molecular, biochemical, and histopathological alterations
Information on the biological responses of polyploid animals towards environmental contaminants is scarce. This study aimed to compare reproductive axis-related gene expressions in the brain, plasma biochemical responses, and the liver and gill histopathological alterations in diploid and triploid f...
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35198/1/Acute%20phenanthrene%20toxicity%20to%20juvenile%20diploid%20and%20triploid%20African%20catfish%20%28Clarias%20gariepinus%29%20molecular%2C%20biochemical%2C%20and%20histopathological%20alterations.pdf |
| _version_ | 1796972659350700032 |
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| author | Karami, Ali Romano, Nicholas Paul Hamzah, Hazilawati Simpson, Stuart L. Yap, Chee Kong |
| author_facet | Karami, Ali Romano, Nicholas Paul Hamzah, Hazilawati Simpson, Stuart L. Yap, Chee Kong |
| author_sort | Karami, Ali |
| collection | UPM |
| description | Information on the biological responses of polyploid animals towards environmental contaminants is scarce. This study aimed to compare reproductive axis-related gene expressions in the brain, plasma biochemical responses, and the liver and gill histopathological alterations in diploid and triploid full-sibling juvenile African catfish (Clarias gariepinus). Fish were exposed for 96 h to one of the two waterborne phenanthrene (Phe) concentrations [mean measured (SD): 6.2 (2.4) and 76 (4.2) μg/L]. In triploids, exposure to 76 μg/L Phe increased mRNA level of fushi tarazu-factor 1 (ftz-f1). Expression of tryptophan hydroxylase2 (tph2) was also elevated in both ploidies following the exposure to 76 μg/L Phe compared to the solvent control. In triploids, 76 μg/L Phe increased plasma alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) levels compared to the other Phe-exposed group. It also elevated lactate and glucose contents relative to the other groups. In diploids, however, biochemical biomarkers did not change. Phenanthrene exposures elevated glycogen contents and the prevalence of histopathological lesions in the liver and gills of both ploidies. This study showed substantial differences between diploids and triploids on biochemical and molecular biomarker responses, but similar histopathological alterations following acute Phe exposures. |
| first_indexed | 2024-03-06T08:31:36Z |
| format | Article |
| id | upm.eprints-35198 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:31:36Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-351982016-10-11T02:54:47Z http://psasir.upm.edu.my/id/eprint/35198/ Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): molecular, biochemical, and histopathological alterations Karami, Ali Romano, Nicholas Paul Hamzah, Hazilawati Simpson, Stuart L. Yap, Chee Kong Information on the biological responses of polyploid animals towards environmental contaminants is scarce. This study aimed to compare reproductive axis-related gene expressions in the brain, plasma biochemical responses, and the liver and gill histopathological alterations in diploid and triploid full-sibling juvenile African catfish (Clarias gariepinus). Fish were exposed for 96 h to one of the two waterborne phenanthrene (Phe) concentrations [mean measured (SD): 6.2 (2.4) and 76 (4.2) μg/L]. In triploids, exposure to 76 μg/L Phe increased mRNA level of fushi tarazu-factor 1 (ftz-f1). Expression of tryptophan hydroxylase2 (tph2) was also elevated in both ploidies following the exposure to 76 μg/L Phe compared to the solvent control. In triploids, 76 μg/L Phe increased plasma alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) levels compared to the other Phe-exposed group. It also elevated lactate and glucose contents relative to the other groups. In diploids, however, biochemical biomarkers did not change. Phenanthrene exposures elevated glycogen contents and the prevalence of histopathological lesions in the liver and gills of both ploidies. This study showed substantial differences between diploids and triploids on biochemical and molecular biomarker responses, but similar histopathological alterations following acute Phe exposures. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35198/1/Acute%20phenanthrene%20toxicity%20to%20juvenile%20diploid%20and%20triploid%20African%20catfish%20%28Clarias%20gariepinus%29%20molecular%2C%20biochemical%2C%20and%20histopathological%20alterations.pdf Karami, Ali and Romano, Nicholas Paul and Hamzah, Hazilawati and Simpson, Stuart L. and Yap, Chee Kong (2016) Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): molecular, biochemical, and histopathological alterations. Environmental Pollution, 212. pp. 155-165. ISSN 0269-7491; ESSN: 1873-6424 http://www.sciencedirect.com/science/article/pii/S0269749116300550 10.1016/j.envpol.2016.01.055 |
| spellingShingle | Karami, Ali Romano, Nicholas Paul Hamzah, Hazilawati Simpson, Stuart L. Yap, Chee Kong Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): molecular, biochemical, and histopathological alterations |
| title | Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): molecular, biochemical, and histopathological alterations |
| title_full | Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): molecular, biochemical, and histopathological alterations |
| title_fullStr | Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): molecular, biochemical, and histopathological alterations |
| title_full_unstemmed | Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): molecular, biochemical, and histopathological alterations |
| title_short | Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): molecular, biochemical, and histopathological alterations |
| title_sort | acute phenanthrene toxicity to juvenile diploid and triploid african catfish clarias gariepinus molecular biochemical and histopathological alterations |
| url | http://psasir.upm.edu.my/id/eprint/35198/1/Acute%20phenanthrene%20toxicity%20to%20juvenile%20diploid%20and%20triploid%20African%20catfish%20%28Clarias%20gariepinus%29%20molecular%2C%20biochemical%2C%20and%20histopathological%20alterations.pdf |
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