Species delineation of Malaysian mangrove fireflies (Coleoptera: Lampyridae) using DNA barcodes
Identification and revision of firefly species are largely based on morphological characteristics. Subsequently, female firefly specimens have been neglected in taxonomic inventories because morphological descriptions relied only on male firefly specimens. In light of successful DNA barcoding in man...
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| Format: | Article |
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BioOne
2014
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| _version_ | 1825948646080774144 |
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| author | Jusoh, Wan Faridah Akmal Hashim, Nor Rasidah Saaksjarvi, Ilari Eerikki Adam, Nur Azura Wahlberg, Niklas |
| author_facet | Jusoh, Wan Faridah Akmal Hashim, Nor Rasidah Saaksjarvi, Ilari Eerikki Adam, Nur Azura Wahlberg, Niklas |
| author_sort | Jusoh, Wan Faridah Akmal |
| collection | UPM |
| description | Identification and revision of firefly species are largely based on morphological characteristics. Subsequently, female firefly specimens have been neglected in taxonomic inventories because morphological descriptions relied only on male firefly specimens. In light of successful DNA barcoding in many invertebrates, this study explored the potential of DNA barcoding to complement morphological identification of fireflies in Malaysia. Firefly specimens were collected from riparian forests (including mangroves) in Peninsular Malaysia and Sarawak. Specimens were morphologically examined, and their DNA was extracted for molecular analysis. The morphological data of the male specimens was found to be congruent with molecular data, i.e., both indicated 12 species. However, inclusion of females and larvae in the DNA analysis increased the species count to 14 species. We also found distinct geographically structured variations in the DNA barcodes of two species: Pteroptyx tener Olivier and Pteroptyx bearni Olivier. The study indicates that firefly species delimitation using traditional taxonomic knowledge and new technology like DNA barcoding are compatible, therefore increasing the reliability of species identification. |
| first_indexed | 2024-03-06T08:32:19Z |
| format | Article |
| id | upm.eprints-35434 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:32:19Z |
| publishDate | 2014 |
| publisher | BioOne |
| record_format | dspace |
| spelling | upm.eprints-354342016-01-11T01:46:07Z http://psasir.upm.edu.my/id/eprint/35434/ Species delineation of Malaysian mangrove fireflies (Coleoptera: Lampyridae) using DNA barcodes Jusoh, Wan Faridah Akmal Hashim, Nor Rasidah Saaksjarvi, Ilari Eerikki Adam, Nur Azura Wahlberg, Niklas Identification and revision of firefly species are largely based on morphological characteristics. Subsequently, female firefly specimens have been neglected in taxonomic inventories because morphological descriptions relied only on male firefly specimens. In light of successful DNA barcoding in many invertebrates, this study explored the potential of DNA barcoding to complement morphological identification of fireflies in Malaysia. Firefly specimens were collected from riparian forests (including mangroves) in Peninsular Malaysia and Sarawak. Specimens were morphologically examined, and their DNA was extracted for molecular analysis. The morphological data of the male specimens was found to be congruent with molecular data, i.e., both indicated 12 species. However, inclusion of females and larvae in the DNA analysis increased the species count to 14 species. We also found distinct geographically structured variations in the DNA barcodes of two species: Pteroptyx tener Olivier and Pteroptyx bearni Olivier. The study indicates that firefly species delimitation using traditional taxonomic knowledge and new technology like DNA barcoding are compatible, therefore increasing the reliability of species identification. BioOne 2014 Article PeerReviewed Jusoh, Wan Faridah Akmal and Hashim, Nor Rasidah and Saaksjarvi, Ilari Eerikki and Adam, Nur Azura and Wahlberg, Niklas (2014) Species delineation of Malaysian mangrove fireflies (Coleoptera: Lampyridae) using DNA barcodes. The Coleopterists Bulletin, 68 (4). pp. 703-711. ISSN 0010-065X; ESSN: 1938-4394 http://www.bioone.org/doi/abs/10.1649/0010-065X-68.4.703 10.1649/0010-065X-68.4.703 |
| spellingShingle | Jusoh, Wan Faridah Akmal Hashim, Nor Rasidah Saaksjarvi, Ilari Eerikki Adam, Nur Azura Wahlberg, Niklas Species delineation of Malaysian mangrove fireflies (Coleoptera: Lampyridae) using DNA barcodes |
| title | Species delineation of Malaysian mangrove fireflies (Coleoptera: Lampyridae) using DNA barcodes |
| title_full | Species delineation of Malaysian mangrove fireflies (Coleoptera: Lampyridae) using DNA barcodes |
| title_fullStr | Species delineation of Malaysian mangrove fireflies (Coleoptera: Lampyridae) using DNA barcodes |
| title_full_unstemmed | Species delineation of Malaysian mangrove fireflies (Coleoptera: Lampyridae) using DNA barcodes |
| title_short | Species delineation of Malaysian mangrove fireflies (Coleoptera: Lampyridae) using DNA barcodes |
| title_sort | species delineation of malaysian mangrove fireflies coleoptera lampyridae using dna barcodes |
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