Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass
The combination of superheated steam (SHS) with ligninolytic enzyme laccase pretreatment together with size reduction was conducted in order to enhance the enzymatic hydrolysis of oil palm biomass into glucose. The oil palm empty fruit bunch (OPEFB) and oil palm mesocarp fiber (OPMF) were pretreated...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35496/1/35496.pdf |
| _version_ | 1825948658206507008 |
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| author | Ahmad Rizal, Nur Fatin Athirah Ibrahim, Mohamad Faizal Zakaria @ Mamat, Mohd Rafein Kamal Bahrin, Ezyana Abd. Aziz, Suraini Hassan, Mohd Ali |
| author_facet | Ahmad Rizal, Nur Fatin Athirah Ibrahim, Mohamad Faizal Zakaria @ Mamat, Mohd Rafein Kamal Bahrin, Ezyana Abd. Aziz, Suraini Hassan, Mohd Ali |
| author_sort | Ahmad Rizal, Nur Fatin Athirah |
| collection | UPM |
| description | The combination of superheated steam (SHS) with ligninolytic enzyme laccase pretreatment together with size reduction was conducted in order to enhance the enzymatic hydrolysis of oil palm biomass into glucose. The oil palm empty fruit bunch (OPEFB) and oil palm mesocarp fiber (OPMF) were pretreated with SHS and ground using a hammer mill to sizes of 2, 1, 0.5 and 0.25 mm before pretreatment using laccase to remove lignin. This study showed that reduction of size from raw to 0.25 mm plays important role in lignin degradation by laccase that removed 38.7% and 39.6% of the lignin from OPEFB and OPMF, respectively. The subsequent saccharification process of these pretreated OPEFB and OPMF generates glucose yields of 71.5% and 63.0%, which represent a 4.6 and 4.8-fold increase, respectively, as compared to untreated samples. This study showed that the combination of SHS with laccase pretreatment together with size reduction could enhance the glucose yield. |
| first_indexed | 2024-03-06T08:32:30Z |
| format | Article |
| id | upm.eprints-35496 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:32:30Z |
| publishDate | 2018 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-354962018-08-13T01:35:19Z http://psasir.upm.edu.my/id/eprint/35496/ Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass Ahmad Rizal, Nur Fatin Athirah Ibrahim, Mohamad Faizal Zakaria @ Mamat, Mohd Rafein Kamal Bahrin, Ezyana Abd. Aziz, Suraini Hassan, Mohd Ali The combination of superheated steam (SHS) with ligninolytic enzyme laccase pretreatment together with size reduction was conducted in order to enhance the enzymatic hydrolysis of oil palm biomass into glucose. The oil palm empty fruit bunch (OPEFB) and oil palm mesocarp fiber (OPMF) were pretreated with SHS and ground using a hammer mill to sizes of 2, 1, 0.5 and 0.25 mm before pretreatment using laccase to remove lignin. This study showed that reduction of size from raw to 0.25 mm plays important role in lignin degradation by laccase that removed 38.7% and 39.6% of the lignin from OPEFB and OPMF, respectively. The subsequent saccharification process of these pretreated OPEFB and OPMF generates glucose yields of 71.5% and 63.0%, which represent a 4.6 and 4.8-fold increase, respectively, as compared to untreated samples. This study showed that the combination of SHS with laccase pretreatment together with size reduction could enhance the glucose yield. MDPI 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/35496/1/35496.pdf Ahmad Rizal, Nur Fatin Athirah and Ibrahim, Mohamad Faizal and Zakaria @ Mamat, Mohd Rafein and Kamal Bahrin, Ezyana and Abd. Aziz, Suraini and Hassan, Mohd Ali (2018) Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass. Molecules, 23 (4). art. no. 811. pp. 1-14. ISSN 1420-3049 http://www.mdpi.com/1420-3049/23/4/811 10.3390/molecules23040811 |
| spellingShingle | Ahmad Rizal, Nur Fatin Athirah Ibrahim, Mohamad Faizal Zakaria @ Mamat, Mohd Rafein Kamal Bahrin, Ezyana Abd. Aziz, Suraini Hassan, Mohd Ali Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass |
| title | Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass |
| title_full | Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass |
| title_fullStr | Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass |
| title_full_unstemmed | Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass |
| title_short | Combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass |
| title_sort | combination of superheated steam with laccase pretreatment together with size reduction to enhance enzymatic hydrolysis of oil palm biomass |
| url | http://psasir.upm.edu.my/id/eprint/35496/1/35496.pdf |
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