The assessment of cholinesterase from the liver of Puntius Javanicus as detection of metal ions
Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 µmole/min/mg and 0.23 mM...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35629/1/The%20Assessment%20of%20Cholinesterase%20from%20the%20Liver%20of.pdf |
| _version_ | 1825948684590776320 |
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| author | Sabullah, Mohd Khalizan Sulaiman, Mohd Rosni Abd Shukor, Mohd Yunus Syed, Mohd Arif Shamaan, Nor Aripin Khalid, Ariff Ahmad, Siti Aqlima |
| author_facet | Sabullah, Mohd Khalizan Sulaiman, Mohd Rosni Abd Shukor, Mohd Yunus Syed, Mohd Arif Shamaan, Nor Aripin Khalid, Ariff Ahmad, Siti Aqlima |
| author_sort | Sabullah, Mohd Khalizan |
| collection | UPM |
| description | Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 µmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23. The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C. The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature. However, ChE activity showed no significant difference when stored at 4.0, 0, and −25°C for 15 days. Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P. javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury. The results showed that P. javanicus ChE has the potential to be used as a biosensor for the detection of metal ions. |
| first_indexed | 2024-03-06T08:32:54Z |
| format | Article |
| id | upm.eprints-35629 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:32:54Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-356292016-01-18T03:25:01Z http://psasir.upm.edu.my/id/eprint/35629/ The assessment of cholinesterase from the liver of Puntius Javanicus as detection of metal ions Sabullah, Mohd Khalizan Sulaiman, Mohd Rosni Abd Shukor, Mohd Yunus Syed, Mohd Arif Shamaan, Nor Aripin Khalid, Ariff Ahmad, Siti Aqlima Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 µmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23. The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C. The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature. However, ChE activity showed no significant difference when stored at 4.0, 0, and −25°C for 15 days. Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P. javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury. The results showed that P. javanicus ChE has the potential to be used as a biosensor for the detection of metal ions. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35629/1/The%20Assessment%20of%20Cholinesterase%20from%20the%20Liver%20of.pdf Sabullah, Mohd Khalizan and Sulaiman, Mohd Rosni and Abd Shukor, Mohd Yunus and Syed, Mohd Arif and Shamaan, Nor Aripin and Khalid, Ariff and Ahmad, Siti Aqlima (2014) The assessment of cholinesterase from the liver of Puntius Javanicus as detection of metal ions. The Scientific World Journal, 2014. art. no. 571094. pp. 1-9. ISSN 2356-6140; ESSN: 1537-744X http://www.hindawi.com/journals/tswj/2014/571094/abs/ 10.1155/2014/571094 |
| spellingShingle | Sabullah, Mohd Khalizan Sulaiman, Mohd Rosni Abd Shukor, Mohd Yunus Syed, Mohd Arif Shamaan, Nor Aripin Khalid, Ariff Ahmad, Siti Aqlima The assessment of cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title | The assessment of cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_full | The assessment of cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_fullStr | The assessment of cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_full_unstemmed | The assessment of cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_short | The assessment of cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_sort | assessment of cholinesterase from the liver of puntius javanicus as detection of metal ions |
| url | http://psasir.upm.edu.my/id/eprint/35629/1/The%20Assessment%20of%20Cholinesterase%20from%20the%20Liver%20of.pdf |
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