Uptake and accumulation of aluminium, copper and cobalt in tissue cultured Melastoma malabathricum Linn. plantlets

Aims: This study was carried out to investigate the metal tolerance levels of Melastoma malabathricum Linn. plantlets. Study Design: The metal tolerance levels of M. malabathricum L. were examined using an in vitro approach. The ability of the plant to survive in tissue culture medium containing al...

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Main Authors: Thau, Wilson Lym Yong, Li, Joan Jett Chang, Thien, Vun Yee, Lye, Harry Hin Chong, Abdullah, Janna Ong
Format: Article
Published: SCIENCEDOMAIN international 2014
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author Thau, Wilson Lym Yong
Li, Joan Jett Chang
Thien, Vun Yee
Lye, Harry Hin Chong
Abdullah, Janna Ong
author_facet Thau, Wilson Lym Yong
Li, Joan Jett Chang
Thien, Vun Yee
Lye, Harry Hin Chong
Abdullah, Janna Ong
author_sort Thau, Wilson Lym Yong
collection UPM
description Aims: This study was carried out to investigate the metal tolerance levels of Melastoma malabathricum Linn. plantlets. Study Design: The metal tolerance levels of M. malabathricum L. were examined using an in vitro approach. The ability of the plant to survive in tissue culture medium containing aluminium (Al), copper (Cu) and cobalt (Co) were assessed. Place and Duration of Study: The study was carried out in the Plant Culture Laboratory, Biotechnology Research Institute, Universiti Malaysia Sabah (UMS), between September 2011 and February 2013. Methodology: M. malabathricum L. plantlets were cultured on half strength MS media supplemented with Al, Cu and Co at concentrations ranging from 0, 0.5, 1.0, 1.5 to 2.0 mM. The growth and survival of the plantlets were observed at every 10 days of treatment and the metals accumulation levels in the leaves and roots were analyzed after 30 days of culture. Results: The order of the survival rate for M. malabathricum L. plantlets subjected to these three metals was demonstrated to be Al > Cu > Co in the highest metal concentration tested. More accumulation of Al was observed in the roots, and Cu was found to be higher than Co in the leaves. Conclusion: Data obtained from this study on the potential uptake and accumulation of toxic metals for M. malabathricum L. will be used in future for the development of this plant species as a phytoremediator.
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spelling upm.eprints-359272016-02-11T08:46:31Z http://psasir.upm.edu.my/id/eprint/35927/ Uptake and accumulation of aluminium, copper and cobalt in tissue cultured Melastoma malabathricum Linn. plantlets Thau, Wilson Lym Yong Li, Joan Jett Chang Thien, Vun Yee Lye, Harry Hin Chong Abdullah, Janna Ong Aims: This study was carried out to investigate the metal tolerance levels of Melastoma malabathricum Linn. plantlets. Study Design: The metal tolerance levels of M. malabathricum L. were examined using an in vitro approach. The ability of the plant to survive in tissue culture medium containing aluminium (Al), copper (Cu) and cobalt (Co) were assessed. Place and Duration of Study: The study was carried out in the Plant Culture Laboratory, Biotechnology Research Institute, Universiti Malaysia Sabah (UMS), between September 2011 and February 2013. Methodology: M. malabathricum L. plantlets were cultured on half strength MS media supplemented with Al, Cu and Co at concentrations ranging from 0, 0.5, 1.0, 1.5 to 2.0 mM. The growth and survival of the plantlets were observed at every 10 days of treatment and the metals accumulation levels in the leaves and roots were analyzed after 30 days of culture. Results: The order of the survival rate for M. malabathricum L. plantlets subjected to these three metals was demonstrated to be Al > Cu > Co in the highest metal concentration tested. More accumulation of Al was observed in the roots, and Cu was found to be higher than Co in the leaves. Conclusion: Data obtained from this study on the potential uptake and accumulation of toxic metals for M. malabathricum L. will be used in future for the development of this plant species as a phytoremediator. SCIENCEDOMAIN international 2014-08 Article NonPeerReviewed Thau, Wilson Lym Yong and Li, Joan Jett Chang and Thien, Vun Yee and Lye, Harry Hin Chong and Abdullah, Janna Ong (2014) Uptake and accumulation of aluminium, copper and cobalt in tissue cultured Melastoma malabathricum Linn. plantlets. International Journal of Plant & Soil Science, 3 (8). pp. 1018-1030. ISSN 2320-7035 http://sciencedomain.org/abstract/4847 10.9734/IJPSS/2014/10253
spellingShingle Thau, Wilson Lym Yong
Li, Joan Jett Chang
Thien, Vun Yee
Lye, Harry Hin Chong
Abdullah, Janna Ong
Uptake and accumulation of aluminium, copper and cobalt in tissue cultured Melastoma malabathricum Linn. plantlets
title Uptake and accumulation of aluminium, copper and cobalt in tissue cultured Melastoma malabathricum Linn. plantlets
title_full Uptake and accumulation of aluminium, copper and cobalt in tissue cultured Melastoma malabathricum Linn. plantlets
title_fullStr Uptake and accumulation of aluminium, copper and cobalt in tissue cultured Melastoma malabathricum Linn. plantlets
title_full_unstemmed Uptake and accumulation of aluminium, copper and cobalt in tissue cultured Melastoma malabathricum Linn. plantlets
title_short Uptake and accumulation of aluminium, copper and cobalt in tissue cultured Melastoma malabathricum Linn. plantlets
title_sort uptake and accumulation of aluminium copper and cobalt in tissue cultured melastoma malabathricum linn plantlets
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AT lijoanjettchang uptakeandaccumulationofaluminiumcopperandcobaltintissueculturedmelastomamalabathricumlinnplantlets
AT thienvunyee uptakeandaccumulationofaluminiumcopperandcobaltintissueculturedmelastomamalabathricumlinnplantlets
AT lyeharryhinchong uptakeandaccumulationofaluminiumcopperandcobaltintissueculturedmelastomamalabathricumlinnplantlets
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