Antigen expression pattern of acute promyelocytic leukaemia cases in Malaysia
Introduction: Acute Promyelocytic Leukaemia (APL) is associated with devastating coagulopathy and life threatening condition which requires immediate medical attention. It is crucial to establish an expedited diagnosis as early therapeutic intervention has led to optimal patient management. In this...
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| Format: | Article |
| Language: | English |
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Malaysian Medical Association
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36475/1/Antigen%20expression%20pattern%20of%20acute%20promyelocytic%20leukaemia%20cases%20in%20Malaysia.pdf |
| _version_ | 1825948848974987264 |
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| author | Ambayya, Angeli Seman, Zainina Md Said, Salmiah Md Noor, Sabariah |
| author_facet | Ambayya, Angeli Seman, Zainina Md Said, Salmiah Md Noor, Sabariah |
| author_sort | Ambayya, Angeli |
| collection | UPM |
| description | Introduction: Acute Promyelocytic Leukaemia (APL) is associated with devastating coagulopathy and life threatening condition which requires immediate medical attention. It is crucial to establish an expedited diagnosis as early therapeutic intervention has led to optimal patient management. In this study, we assessed the type and frequency of antigen expressions in APL and correlated these findings with genetic studies. Methods: Multiparametric immunophenotyping was performed on 30 samples and findings were correlated with karyotypes, FISH for t(15;17) translocation and RT-PCR for PML-RARα for detection of breakpoint cluster regions (bcr1,bcr2 and bcr3). Results: On SSC/CD45, APL cells displayed high to moderate SSC, with the expression of CD33 (100%), CD13 (96.8%), cMPO (71%) but lacked CD34 (3.2%) and HLA-DR (9.7%). Aberrant expression of CD4 was seen in 12.9% and CD56 in 6.5% of the cases. A significant association between cumulative aberrant antigen expression and bcr1 were observed bcr1 (X2(2) =6.833,p<.05). However there were no significant association seen in bcr2 and bcr3; (X2(2) =.199,p>.05) and (X2(2)=4.599,p>.05) respectively. Conclusions: Flow cytometry is a rapid and effective tool in detecting APL. It is interesting to note that there is significant association between cumulative aberrant antigen expression and genotype analysis. Further validation is required to corroborate this relationship. |
| first_indexed | 2024-03-06T08:35:26Z |
| format | Article |
| id | upm.eprints-36475 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:35:26Z |
| publishDate | 2014 |
| publisher | Malaysian Medical Association |
| record_format | dspace |
| spelling | upm.eprints-364752015-08-24T03:29:20Z http://psasir.upm.edu.my/id/eprint/36475/ Antigen expression pattern of acute promyelocytic leukaemia cases in Malaysia Ambayya, Angeli Seman, Zainina Md Said, Salmiah Md Noor, Sabariah Introduction: Acute Promyelocytic Leukaemia (APL) is associated with devastating coagulopathy and life threatening condition which requires immediate medical attention. It is crucial to establish an expedited diagnosis as early therapeutic intervention has led to optimal patient management. In this study, we assessed the type and frequency of antigen expressions in APL and correlated these findings with genetic studies. Methods: Multiparametric immunophenotyping was performed on 30 samples and findings were correlated with karyotypes, FISH for t(15;17) translocation and RT-PCR for PML-RARα for detection of breakpoint cluster regions (bcr1,bcr2 and bcr3). Results: On SSC/CD45, APL cells displayed high to moderate SSC, with the expression of CD33 (100%), CD13 (96.8%), cMPO (71%) but lacked CD34 (3.2%) and HLA-DR (9.7%). Aberrant expression of CD4 was seen in 12.9% and CD56 in 6.5% of the cases. A significant association between cumulative aberrant antigen expression and bcr1 were observed bcr1 (X2(2) =6.833,p<.05). However there were no significant association seen in bcr2 and bcr3; (X2(2) =.199,p>.05) and (X2(2)=4.599,p>.05) respectively. Conclusions: Flow cytometry is a rapid and effective tool in detecting APL. It is interesting to note that there is significant association between cumulative aberrant antigen expression and genotype analysis. Further validation is required to corroborate this relationship. Malaysian Medical Association 2014-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36475/1/Antigen%20expression%20pattern%20of%20acute%20promyelocytic%20leukaemia%20cases%20in%20Malaysia.pdf Ambayya, Angeli and Seman, Zainina and Md Said, Salmiah and Md Noor, Sabariah (2014) Antigen expression pattern of acute promyelocytic leukaemia cases in Malaysia. Medical Journal of Malaysia, 69 (2). pp. 64-69. ISSN 0300-5283 http://www.e-mjm.org/2014/v69n2/acute-promyelocytic-leukaemia.pdf |
| spellingShingle | Ambayya, Angeli Seman, Zainina Md Said, Salmiah Md Noor, Sabariah Antigen expression pattern of acute promyelocytic leukaemia cases in Malaysia |
| title | Antigen expression pattern of acute promyelocytic leukaemia cases in Malaysia |
| title_full | Antigen expression pattern of acute promyelocytic leukaemia cases in Malaysia |
| title_fullStr | Antigen expression pattern of acute promyelocytic leukaemia cases in Malaysia |
| title_full_unstemmed | Antigen expression pattern of acute promyelocytic leukaemia cases in Malaysia |
| title_short | Antigen expression pattern of acute promyelocytic leukaemia cases in Malaysia |
| title_sort | antigen expression pattern of acute promyelocytic leukaemia cases in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/36475/1/Antigen%20expression%20pattern%20of%20acute%20promyelocytic%20leukaemia%20cases%20in%20Malaysia.pdf |
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