Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens

The antimicrobial activity of Cosmos caudatus extract was evaluated against Bacillus cereus(ATCC 33019), Bacillus subtilis(ATCC 6633), Proteus mirabilis (ATCC 21100), Pseudomonas aeruginosa (ATCC 9027) and Candida albicans (ATCC 10231) using the methods as recommended by the Clinical and Laboratory...

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Main Authors: Yusoff, N. A. H., Bustamam, M. S., Abas, Faridah, Khatib, Alfi, Rukayadi, Yaya
Format: Article
Language:English
Published: Oriental Scientific Publishing 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36479/1/Antimicrobial%20activity%20of%20Cosmos%20caudatus%20extract%20against%20foodborne%20pathogens.pdf
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author Yusoff, N. A. H.
Bustamam, M. S.
Abas, Faridah
Khatib, Alfi
Rukayadi, Yaya
author_facet Yusoff, N. A. H.
Bustamam, M. S.
Abas, Faridah
Khatib, Alfi
Rukayadi, Yaya
author_sort Yusoff, N. A. H.
collection UPM
description The antimicrobial activity of Cosmos caudatus extract was evaluated against Bacillus cereus(ATCC 33019), Bacillus subtilis(ATCC 6633), Proteus mirabilis (ATCC 21100), Pseudomonas aeruginosa (ATCC 9027) and Candida albicans (ATCC 10231) using the methods as recommended by the Clinical and Laboratory Standard Institute (CLSI). The antimicrobial tests were conducted in term of susceptibility, minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration (MBC/MFC) and killing-time curve. The results showed that C. caudatus extract was susceptible against all tested pathogens; the inhibition zone ranged from 8.60 mm to 9.83 mm. The MIC and MBC/MFC values were ranged from 6.25 mg/ml-12.50 mg/ml and 12.50 mg/ml-50.00 mg/ml, respectively. Mean while, killing-time curves showed that C. caudatus extract can killed the B. cereus, B. subtilis, P. mirabilis, P. aeruginosa and C. albicans at concentration of 8 MIC for 2 h, 4 MIC for 2 h or 2 MIC for 2 h, 8 MIC for 4 h, 4 MIC for 0.5 h and 4 MIC for 1 h, as respectively. Findings indicated that C. caudatus extract has the potentiality to develop as a natural antimicrobial agent.
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spelling upm.eprints-364792015-10-22T03:27:32Z http://psasir.upm.edu.my/id/eprint/36479/ Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens Yusoff, N. A. H. Bustamam, M. S. Abas, Faridah Khatib, Alfi Rukayadi, Yaya The antimicrobial activity of Cosmos caudatus extract was evaluated against Bacillus cereus(ATCC 33019), Bacillus subtilis(ATCC 6633), Proteus mirabilis (ATCC 21100), Pseudomonas aeruginosa (ATCC 9027) and Candida albicans (ATCC 10231) using the methods as recommended by the Clinical and Laboratory Standard Institute (CLSI). The antimicrobial tests were conducted in term of susceptibility, minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration (MBC/MFC) and killing-time curve. The results showed that C. caudatus extract was susceptible against all tested pathogens; the inhibition zone ranged from 8.60 mm to 9.83 mm. The MIC and MBC/MFC values were ranged from 6.25 mg/ml-12.50 mg/ml and 12.50 mg/ml-50.00 mg/ml, respectively. Mean while, killing-time curves showed that C. caudatus extract can killed the B. cereus, B. subtilis, P. mirabilis, P. aeruginosa and C. albicans at concentration of 8 MIC for 2 h, 4 MIC for 2 h or 2 MIC for 2 h, 8 MIC for 4 h, 4 MIC for 0.5 h and 4 MIC for 1 h, as respectively. Findings indicated that C. caudatus extract has the potentiality to develop as a natural antimicrobial agent. Oriental Scientific Publishing 2014-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36479/1/Antimicrobial%20activity%20of%20Cosmos%20caudatus%20extract%20against%20foodborne%20pathogens.pdf Yusoff, N. A. H. and Bustamam, M. S. and Abas, Faridah and Khatib, Alfi and Rukayadi, Yaya (2014) Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens. Journal of Pure and Applied Microbiology, 8 (5). pp. 3681-3688. ISSN 0973-7510 http://www.microbiologyjournal.org/jmabsread.php?snoid=2142&month=Oct&year=2014
spellingShingle Yusoff, N. A. H.
Bustamam, M. S.
Abas, Faridah
Khatib, Alfi
Rukayadi, Yaya
Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens
title Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens
title_full Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens
title_fullStr Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens
title_full_unstemmed Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens
title_short Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens
title_sort antimicrobial activity of cosmos caudatus extract against foodborne pathogens
url http://psasir.upm.edu.my/id/eprint/36479/1/Antimicrobial%20activity%20of%20Cosmos%20caudatus%20extract%20against%20foodborne%20pathogens.pdf
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