Application of PCR-ELISA in molecular diagnosis
Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36517/1/Application%20of%20PCR.pdf |
| _version_ | 1825948857038536704 |
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| author | Sue, Mei Jean Yeap, Swee Keong Omar, Abdul Rahman Tan, Sheau Wei |
| author_facet | Sue, Mei Jean Yeap, Swee Keong Omar, Abdul Rahman Tan, Sheau Wei |
| author_sort | Sue, Mei Jean |
| collection | UPM |
| description | Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality. |
| first_indexed | 2024-03-06T08:35:33Z |
| format | Article |
| id | upm.eprints-36517 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:35:33Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-365172016-01-26T07:50:42Z http://psasir.upm.edu.my/id/eprint/36517/ Application of PCR-ELISA in molecular diagnosis Sue, Mei Jean Yeap, Swee Keong Omar, Abdul Rahman Tan, Sheau Wei Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36517/1/Application%20of%20PCR.pdf Sue, Mei Jean and Yeap, Swee Keong and Omar, Abdul Rahman and Tan, Sheau Wei (2014) Application of PCR-ELISA in molecular diagnosis. BioMed Research International, 2014. art. no. 653014. pp. 1-6. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2014/653014/ 10.1155/2014/653014 |
| spellingShingle | Sue, Mei Jean Yeap, Swee Keong Omar, Abdul Rahman Tan, Sheau Wei Application of PCR-ELISA in molecular diagnosis |
| title | Application of PCR-ELISA in molecular diagnosis |
| title_full | Application of PCR-ELISA in molecular diagnosis |
| title_fullStr | Application of PCR-ELISA in molecular diagnosis |
| title_full_unstemmed | Application of PCR-ELISA in molecular diagnosis |
| title_short | Application of PCR-ELISA in molecular diagnosis |
| title_sort | application of pcr elisa in molecular diagnosis |
| url | http://psasir.upm.edu.my/id/eprint/36517/1/Application%20of%20PCR.pdf |
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