Arginine–chitosan- and arginine–polyethylene glycol-conjugated superparamagnetic nanoparticles: preparation, cytotoxicity and controlled-release
Iron oxide magnetic nanoparticles (MNPs) can be used in targeted drug delivery systems for localized cancer treatment. MNPs coated with biocompatible polymers are useful for delivering anticancer drugs. Iron oxide MNPs were synthesized via co-precipitation method then coated with either chitosan (CS...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sage Publications
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36535/1/Arginine.pdf |
| _version_ | 1825948860659269632 |
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| author | Hussein Al-Ali, Samer Hasan Arulselvan, Palanisamy Fakurazi, Sharida Hussein, Mohd Zobir Dorniani, Dena |
| author_facet | Hussein Al-Ali, Samer Hasan Arulselvan, Palanisamy Fakurazi, Sharida Hussein, Mohd Zobir Dorniani, Dena |
| author_sort | Hussein Al-Ali, Samer Hasan |
| collection | UPM |
| description | Iron oxide magnetic nanoparticles (MNPs) can be used in targeted drug delivery systems for localized cancer treatment. MNPs coated with biocompatible polymers are useful for delivering anticancer drugs. Iron oxide MNPs were synthesized via co-precipitation method then coated with either chitosan (CS) or polyethylene glycol (PEG) to form CS–MNPs and PEG–MNPs, respectively. Arginine (Arg) was loaded onto both coated nanoparticles to form Arg–CS–MNP and Arg–PEG–MNP nanocomposites. The X-ray diffraction results for the MNPs and the Arg–CS–MNP and Arg–PEG–MNPs nanocomposites indicated that the iron oxide contained pure magnetite. The amount of CS and PEG bound to the MNPs were estimated via thermogravimetric analysis and confirmed via Fourier transform infrared spectroscopy analysis. Arg loading was estimated using UV–vis measurements, which yielded values of 5.5% and 11% for the Arg–CS–MNP and Arg–PEG–MNP nanocomposites, respectively. The release profile of Arg from the nanocomposites followed a pseudo-second-order kinetic model. The cytotoxic effects of the MNPs, Arg–CS–MNPs, and Arg–PEG–MNPs were evaluated in human cervical carcinoma cells (HeLa), mouse embryonic fibroblast cells (3T3) and breast adenocarcinoma cells (MCF-7). The results indicate that the MNPs, Arg–CS–MNPs, and Arg–PEG–MNPs do not exhibit cytotoxicity toward 3T3 and HeLa cells. However, treatment of the MCF-7 cells with the Arg–CS–MNP and Arg–PEG–MNP nanocomposites reduced the cancer cell viability with IC50 values of 48.6 and 42.6 µg/mL, respectively, whereas the MNPs and free Arg did not affect the viability of the MCF-7 cells. |
| first_indexed | 2024-03-06T08:35:37Z |
| format | Article |
| id | upm.eprints-36535 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:35:37Z |
| publishDate | 2014 |
| publisher | Sage Publications |
| record_format | dspace |
| spelling | upm.eprints-365352015-09-10T04:43:33Z http://psasir.upm.edu.my/id/eprint/36535/ Arginine–chitosan- and arginine–polyethylene glycol-conjugated superparamagnetic nanoparticles: preparation, cytotoxicity and controlled-release Hussein Al-Ali, Samer Hasan Arulselvan, Palanisamy Fakurazi, Sharida Hussein, Mohd Zobir Dorniani, Dena Iron oxide magnetic nanoparticles (MNPs) can be used in targeted drug delivery systems for localized cancer treatment. MNPs coated with biocompatible polymers are useful for delivering anticancer drugs. Iron oxide MNPs were synthesized via co-precipitation method then coated with either chitosan (CS) or polyethylene glycol (PEG) to form CS–MNPs and PEG–MNPs, respectively. Arginine (Arg) was loaded onto both coated nanoparticles to form Arg–CS–MNP and Arg–PEG–MNP nanocomposites. The X-ray diffraction results for the MNPs and the Arg–CS–MNP and Arg–PEG–MNPs nanocomposites indicated that the iron oxide contained pure magnetite. The amount of CS and PEG bound to the MNPs were estimated via thermogravimetric analysis and confirmed via Fourier transform infrared spectroscopy analysis. Arg loading was estimated using UV–vis measurements, which yielded values of 5.5% and 11% for the Arg–CS–MNP and Arg–PEG–MNP nanocomposites, respectively. The release profile of Arg from the nanocomposites followed a pseudo-second-order kinetic model. The cytotoxic effects of the MNPs, Arg–CS–MNPs, and Arg–PEG–MNPs were evaluated in human cervical carcinoma cells (HeLa), mouse embryonic fibroblast cells (3T3) and breast adenocarcinoma cells (MCF-7). The results indicate that the MNPs, Arg–CS–MNPs, and Arg–PEG–MNPs do not exhibit cytotoxicity toward 3T3 and HeLa cells. However, treatment of the MCF-7 cells with the Arg–CS–MNP and Arg–PEG–MNP nanocomposites reduced the cancer cell viability with IC50 values of 48.6 and 42.6 µg/mL, respectively, whereas the MNPs and free Arg did not affect the viability of the MCF-7 cells. Sage Publications 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36535/1/Arginine.pdf Hussein Al-Ali, Samer Hasan and Arulselvan, Palanisamy and Fakurazi, Sharida and Hussein, Mohd Zobir and Dorniani, Dena (2014) Arginine–chitosan- and arginine–polyethylene glycol-conjugated superparamagnetic nanoparticles: preparation, cytotoxicity and controlled-release. Journal of Biomaterials Applications, 29 (2). pp. 186-198. ISSN 0885-3282; ESSN: 1530-8022 http://jba.sagepub.com/content/29/2/186.abstract 10.1177/0885328213519691 |
| spellingShingle | Hussein Al-Ali, Samer Hasan Arulselvan, Palanisamy Fakurazi, Sharida Hussein, Mohd Zobir Dorniani, Dena Arginine–chitosan- and arginine–polyethylene glycol-conjugated superparamagnetic nanoparticles: preparation, cytotoxicity and controlled-release |
| title | Arginine–chitosan- and arginine–polyethylene glycol-conjugated superparamagnetic nanoparticles: preparation, cytotoxicity and controlled-release |
| title_full | Arginine–chitosan- and arginine–polyethylene glycol-conjugated superparamagnetic nanoparticles: preparation, cytotoxicity and controlled-release |
| title_fullStr | Arginine–chitosan- and arginine–polyethylene glycol-conjugated superparamagnetic nanoparticles: preparation, cytotoxicity and controlled-release |
| title_full_unstemmed | Arginine–chitosan- and arginine–polyethylene glycol-conjugated superparamagnetic nanoparticles: preparation, cytotoxicity and controlled-release |
| title_short | Arginine–chitosan- and arginine–polyethylene glycol-conjugated superparamagnetic nanoparticles: preparation, cytotoxicity and controlled-release |
| title_sort | arginine chitosan and arginine polyethylene glycol conjugated superparamagnetic nanoparticles preparation cytotoxicity and controlled release |
| url | http://psasir.upm.edu.my/id/eprint/36535/1/Arginine.pdf |
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