Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves
The effects of ethanol concentration (0%–100%, v/v), solid-to-solvent ratio (1:10–1:60, w/v) and extraction time (30–180 min) on the extraction of polyphenols from agarwood (Aquilaria crassna) were examined. Total phenolic content (TPC), total flavonoid content (TFC) and total flavanol (TF) assays a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36557/1/Assessment%20of%20extraction%20parameters%20on%20antioxidant%20capacity.pdf |
| _version_ | 1825948864981499904 |
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| author | Tay, Pei Yin Tan, Chin Ping Abas, Faridah Yim, Hip Seng Ho, Chun Wai |
| author_facet | Tay, Pei Yin Tan, Chin Ping Abas, Faridah Yim, Hip Seng Ho, Chun Wai |
| author_sort | Tay, Pei Yin |
| collection | UPM |
| description | The effects of ethanol concentration (0%–100%, v/v), solid-to-solvent ratio (1:10–1:60, w/v) and extraction time (30–180 min) on the extraction of polyphenols from agarwood (Aquilaria crassna) were examined. Total phenolic content (TPC), total flavonoid content (TFC) and total flavanol (TF) assays and HPLC-DAD were used for the determination and quantification of polyphenols, flavanol gallates (epigallocatechin gallate—EGCG and epicatechin gallate—ECG) and a benzophenone (iriflophenone 3-C-β-glucoside) from the crude polyphenol extract (CPE) of A. crassna. 2,2'-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was used to evaluate the antioxidant capacity of the CPE. Experimental results concluded that ethanol concentration and solid-to-solvent ratio had significant effects (p < 0.05) on the yields of polyphenol and antioxidant capacity. Extraction time had an insignificant influence on the recovery of EGCG, ECG and iriflophenone 3-C-β-glucoside, as well as radical scavenging capacity from the CPE. The extraction parameters that exhibited maximum yields were 40% (v/v) ethanol, 1:60 (w/v) for 30 min where the TPC, TFC, TF, DPPH, EGCG, ECG and iriflophenone 3-C-β-glucoside levels achieved were 183.5 mg GAE/g DW, 249.0 mg QE/g DW, 4.9 mg CE/g DW, 93.7%, 29.1 mg EGCG/g DW, 44.3 mg ECG/g DW and 39.9 mg iriflophenone 3-C-β-glucoside/g DW respectively. The IC50 of the CPE was 24.6 mg/L. |
| first_indexed | 2024-03-06T08:35:41Z |
| format | Article |
| id | upm.eprints-36557 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:35:41Z |
| publishDate | 2014 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-365572015-12-01T02:41:39Z http://psasir.upm.edu.my/id/eprint/36557/ Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves Tay, Pei Yin Tan, Chin Ping Abas, Faridah Yim, Hip Seng Ho, Chun Wai The effects of ethanol concentration (0%–100%, v/v), solid-to-solvent ratio (1:10–1:60, w/v) and extraction time (30–180 min) on the extraction of polyphenols from agarwood (Aquilaria crassna) were examined. Total phenolic content (TPC), total flavonoid content (TFC) and total flavanol (TF) assays and HPLC-DAD were used for the determination and quantification of polyphenols, flavanol gallates (epigallocatechin gallate—EGCG and epicatechin gallate—ECG) and a benzophenone (iriflophenone 3-C-β-glucoside) from the crude polyphenol extract (CPE) of A. crassna. 2,2'-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was used to evaluate the antioxidant capacity of the CPE. Experimental results concluded that ethanol concentration and solid-to-solvent ratio had significant effects (p < 0.05) on the yields of polyphenol and antioxidant capacity. Extraction time had an insignificant influence on the recovery of EGCG, ECG and iriflophenone 3-C-β-glucoside, as well as radical scavenging capacity from the CPE. The extraction parameters that exhibited maximum yields were 40% (v/v) ethanol, 1:60 (w/v) for 30 min where the TPC, TFC, TF, DPPH, EGCG, ECG and iriflophenone 3-C-β-glucoside levels achieved were 183.5 mg GAE/g DW, 249.0 mg QE/g DW, 4.9 mg CE/g DW, 93.7%, 29.1 mg EGCG/g DW, 44.3 mg ECG/g DW and 39.9 mg iriflophenone 3-C-β-glucoside/g DW respectively. The IC50 of the CPE was 24.6 mg/L. MDPI 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36557/1/Assessment%20of%20extraction%20parameters%20on%20antioxidant%20capacity.pdf Tay, Pei Yin and Tan, Chin Ping and Abas, Faridah and Yim, Hip Seng and Ho, Chun Wai (2014) Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves. Molecules, 19 (8). pp. 12304-12319. ISSN 1431-5157; ESSN: 1420-3049 http://www.mdpi.com/1420-3049/19/8/12304 10.3390/molecules190812304 |
| spellingShingle | Tay, Pei Yin Tan, Chin Ping Abas, Faridah Yim, Hip Seng Ho, Chun Wai Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves |
| title | Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves |
| title_full | Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves |
| title_fullStr | Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves |
| title_full_unstemmed | Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves |
| title_short | Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves |
| title_sort | assessment of extraction parameters on antioxidant capacity polyphenol content epigallocatechin gallate egcg epicatechin gallate ecg and iriflophenone 3 c β glucoside of agarwood aquilaria crassna young leaves |
| url | http://psasir.upm.edu.my/id/eprint/36557/1/Assessment%20of%20extraction%20parameters%20on%20antioxidant%20capacity.pdf |
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