Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique
A simple and rapid method of DNA microsatellite isolation based on the Random Amplified Microsatellites (RAMs) PCR technique was used in this study. The work presented here is part of a continuous effort in refining and perfecting the technique for more rapid, effective and optimum productivity in s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/3656/1/Rapid_and_Non-radioactive_Detection_Method_of_Microsatellites_in_Mystus.pdf |
| _version_ | 1796966593768456192 |
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| author | Chan, Soon Choy Siraj, Siti Shapor Tan, Soon Guan Mohd Yusoff, Khatijah |
| author_facet | Chan, Soon Choy Siraj, Siti Shapor Tan, Soon Guan Mohd Yusoff, Khatijah |
| author_sort | Chan, Soon Choy |
| collection | UPM |
| description | A simple and rapid method of DNA microsatellite isolation based on the Random Amplified Microsatellites (RAMs) PCR technique was used in this study. The work presented here is part of a continuous effort in refining and perfecting the technique for more rapid, effective and optimum productivity in single locus microsatellite marker development for the River catfish, Mystus nemurus. The current refined protocol for microsatellite isolation was able to detect a total of 135 microsatellite regions resulting in 42 unique genomic sequences being submitted to GenBank. This refined technique is able to reduce the total time required from peR cloning till sequencing specific microsatellite regions to less than three and a half months. |
| first_indexed | 2024-03-06T07:01:45Z |
| format | Article |
| id | upm.eprints-3656 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:01:45Z |
| publishDate | 2005 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-36562016-12-02T02:44:07Z http://psasir.upm.edu.my/id/eprint/3656/ Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique Chan, Soon Choy Siraj, Siti Shapor Tan, Soon Guan Mohd Yusoff, Khatijah A simple and rapid method of DNA microsatellite isolation based on the Random Amplified Microsatellites (RAMs) PCR technique was used in this study. The work presented here is part of a continuous effort in refining and perfecting the technique for more rapid, effective and optimum productivity in single locus microsatellite marker development for the River catfish, Mystus nemurus. The current refined protocol for microsatellite isolation was able to detect a total of 135 microsatellite regions resulting in 42 unique genomic sequences being submitted to GenBank. This refined technique is able to reduce the total time required from peR cloning till sequencing specific microsatellite regions to less than three and a half months. Universiti Putra Malaysia Press 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3656/1/Rapid_and_Non-radioactive_Detection_Method_of_Microsatellites_in_Mystus.pdf Chan, Soon Choy and Siraj, Siti Shapor and Tan, Soon Guan and Mohd Yusoff, Khatijah (2005) Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique. Pertanika Journal of Tropical Agricultural Science, 28 (1). pp. 73-77. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2028%20(1)%20Apr.%202005/JTAS%20Vol.28%20(1)%202005%20(73-77).pdf |
| spellingShingle | Chan, Soon Choy Siraj, Siti Shapor Tan, Soon Guan Mohd Yusoff, Khatijah Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique |
| title | Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique |
| title_full | Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique |
| title_fullStr | Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique |
| title_full_unstemmed | Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique |
| title_short | Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique |
| title_sort | rapid and non radioactive detection method of microsatellites in mystus nemurus a refined technique |
| url | http://psasir.upm.edu.my/id/eprint/3656/1/Rapid_and_Non-radioactive_Detection_Method_of_Microsatellites_in_Mystus.pdf |
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