Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch
Bacterial community structure and biochemical changes during the composting of lignocellulosic oil palm empty bunch (EFB) and palm oil mill effluent (POME) anaerobic sludge were studied by examining the succession of the bacterial community and its association with changes in lignocellulosic compone...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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North Carolina State University
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36608/1/Bacterial%20community%20structure%20and%20biochemical%20changes%20associated%20with%20composting%20of%20lignocellulosic%20oil%20palm%20empty%20fruit%20bunch.pdf |
| _version_ | 1825948875060412416 |
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| author | Mohd Zainudin, Mohd Huzairi Hassan, Mohd Ali Md. Shah, Umi Kalsom Abdullah, Norhani Tokura, Mitsunori Yasueda, Hisashi Shirai, Yoshihito Sakai, Kenji Samsu Baharuddin, Azhari |
| author_facet | Mohd Zainudin, Mohd Huzairi Hassan, Mohd Ali Md. Shah, Umi Kalsom Abdullah, Norhani Tokura, Mitsunori Yasueda, Hisashi Shirai, Yoshihito Sakai, Kenji Samsu Baharuddin, Azhari |
| author_sort | Mohd Zainudin, Mohd Huzairi |
| collection | UPM |
| description | Bacterial community structure and biochemical changes during the composting of lignocellulosic oil palm empty bunch (EFB) and palm oil mill effluent (POME) anaerobic sludge were studied by examining the succession of the bacterial community and its association with changes in lignocellulosic components by denaturing gradient gel electrophoresis (DGGE) and the 16S rRNA gene clone library. During composting, a major reduction in cellulose after 10 days from 50% to 19% and the carbon content from 44% to 27% towards the end of the 40-day composting period were observed. The C/N ratio also decreased. A drastic change in the bacterial community structure and diversity throughout the composting process was clearly observed using PCR-DGGE banding patterns. The bacterial community drastically shifted between the thermophilic and maturing stages. 16s rRNA clones belonging to the genera Bacillus, Exiguobacterium, Desemzia, and Planococcus were the dominant groups throughout composting. The species closely related to Solibacillus silvestris were found to be major contributors to changes in the lignocellulosic component. Clones identified as Thermobacillus xylanilyticus, Brachybacterium faecium, Cellulosimicrobium cellulans, Cellulomonas sp., and Thermobifida fusca, which are known to be lignocellulosic-degrading bacteria, were also detected and are believed to support the lignocellulose degradation. |
| first_indexed | 2024-03-06T08:35:50Z |
| format | Article |
| id | upm.eprints-36608 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:35:50Z |
| publishDate | 2014 |
| publisher | North Carolina State University |
| record_format | dspace |
| spelling | upm.eprints-366082016-09-08T01:44:17Z http://psasir.upm.edu.my/id/eprint/36608/ Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch Mohd Zainudin, Mohd Huzairi Hassan, Mohd Ali Md. Shah, Umi Kalsom Abdullah, Norhani Tokura, Mitsunori Yasueda, Hisashi Shirai, Yoshihito Sakai, Kenji Samsu Baharuddin, Azhari Bacterial community structure and biochemical changes during the composting of lignocellulosic oil palm empty bunch (EFB) and palm oil mill effluent (POME) anaerobic sludge were studied by examining the succession of the bacterial community and its association with changes in lignocellulosic components by denaturing gradient gel electrophoresis (DGGE) and the 16S rRNA gene clone library. During composting, a major reduction in cellulose after 10 days from 50% to 19% and the carbon content from 44% to 27% towards the end of the 40-day composting period were observed. The C/N ratio also decreased. A drastic change in the bacterial community structure and diversity throughout the composting process was clearly observed using PCR-DGGE banding patterns. The bacterial community drastically shifted between the thermophilic and maturing stages. 16s rRNA clones belonging to the genera Bacillus, Exiguobacterium, Desemzia, and Planococcus were the dominant groups throughout composting. The species closely related to Solibacillus silvestris were found to be major contributors to changes in the lignocellulosic component. Clones identified as Thermobacillus xylanilyticus, Brachybacterium faecium, Cellulosimicrobium cellulans, Cellulomonas sp., and Thermobifida fusca, which are known to be lignocellulosic-degrading bacteria, were also detected and are believed to support the lignocellulose degradation. North Carolina State University 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36608/1/Bacterial%20community%20structure%20and%20biochemical%20changes%20associated%20with%20composting%20of%20lignocellulosic%20oil%20palm%20empty%20fruit%20bunch.pdf Mohd Zainudin, Mohd Huzairi and Hassan, Mohd Ali and Md. Shah, Umi Kalsom and Abdullah, Norhani and Tokura, Mitsunori and Yasueda, Hisashi and Shirai, Yoshihito and Sakai, Kenji and Samsu Baharuddin, Azhari (2014) Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch. BioResources, 9 (1). pp. 316-335. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_1_316_Zainudin_Bacterial_Community_Structure_Composting |
| spellingShingle | Mohd Zainudin, Mohd Huzairi Hassan, Mohd Ali Md. Shah, Umi Kalsom Abdullah, Norhani Tokura, Mitsunori Yasueda, Hisashi Shirai, Yoshihito Sakai, Kenji Samsu Baharuddin, Azhari Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch |
| title | Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch |
| title_full | Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch |
| title_fullStr | Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch |
| title_full_unstemmed | Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch |
| title_short | Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch |
| title_sort | bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch |
| url | http://psasir.upm.edu.my/id/eprint/36608/1/Bacterial%20community%20structure%20and%20biochemical%20changes%20associated%20with%20composting%20of%20lignocellulosic%20oil%20palm%20empty%20fruit%20bunch.pdf |
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