Biochemical and molecular characterization of potential phosphate-solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth
A study was conducted to determine the total microbial population, the occurrence of growth promoting bacteria and their beneficial traits in acid sulfate soils. The mechanisms by which the bacteria enhance rice seedlings grown under high Al and low pH stress were investigated. Soils and rice root s...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/36643/1/36643.pdf |
| _version_ | 1825948881742987264 |
|---|---|
| author | Panhwar, Qurban Ali Naher, Umme Aminun Jusop, Shamsuddin Othman, Radziah Abdul Latif, Mohammad Ismail, Mohd Razi |
| author_facet | Panhwar, Qurban Ali Naher, Umme Aminun Jusop, Shamsuddin Othman, Radziah Abdul Latif, Mohammad Ismail, Mohd Razi |
| author_sort | Panhwar, Qurban Ali |
| collection | UPM |
| description | A study was conducted to determine the total microbial population, the occurrence of growth promoting bacteria and their beneficial traits in acid sulfate soils. The mechanisms by which the bacteria enhance rice seedlings grown under high Al and low pH stress were investigated. Soils and rice root samples were randomly collected from four sites in the study area (Kelantan, Malaysia). The topsoil pH and exchangeable Al ranged from 3.3 to 4.7 and 1.24 to 4.25 cmolc kg−1, respectively, which are considered unsuitable for rice production. Total bacterial and actinomycetes population in the acidic soils were found to be higher than fungal populations. A total of 21 phosphate-solubilizing bacteria (PSB) including 19 N2-fixing strains were isolated from the acid sulfate soil. Using 16S rRNA gene sequence analysis, three potential PSB strains based on their beneficial characteristics were identified (Burkholderia thailandensis, Sphingomonas pituitosa and Burkholderia seminalis). The isolated strains were capable of producing indoleacetic acid (IAA) and organic acids that were able to reduce Al availability via a chelation process. These PSB isolates solubilized P (43.65%) existing in the growth media within 72 hours of incubation. Seedling of rice variety, MR 219, grown at pH 4, and with different concentrations of Al (0, 50 and 100 µM) was inoculated with these PSB strains. Results showed that the bacteria increased the pH with a concomitant reduction in Al concentration, which translated into better rice growth. The improved root volume and seedling dry weight of the inoculated plants indicated the potential of these isolates to be used in a bio-fertilizer formulation for rice cultivation on acid sulfate soils. |
| first_indexed | 2024-03-06T08:35:56Z |
| format | Article |
| id | upm.eprints-36643 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:35:56Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | upm.eprints-366432017-08-15T05:52:15Z http://psasir.upm.edu.my/id/eprint/36643/ Biochemical and molecular characterization of potential phosphate-solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth Panhwar, Qurban Ali Naher, Umme Aminun Jusop, Shamsuddin Othman, Radziah Abdul Latif, Mohammad Ismail, Mohd Razi A study was conducted to determine the total microbial population, the occurrence of growth promoting bacteria and their beneficial traits in acid sulfate soils. The mechanisms by which the bacteria enhance rice seedlings grown under high Al and low pH stress were investigated. Soils and rice root samples were randomly collected from four sites in the study area (Kelantan, Malaysia). The topsoil pH and exchangeable Al ranged from 3.3 to 4.7 and 1.24 to 4.25 cmolc kg−1, respectively, which are considered unsuitable for rice production. Total bacterial and actinomycetes population in the acidic soils were found to be higher than fungal populations. A total of 21 phosphate-solubilizing bacteria (PSB) including 19 N2-fixing strains were isolated from the acid sulfate soil. Using 16S rRNA gene sequence analysis, three potential PSB strains based on their beneficial characteristics were identified (Burkholderia thailandensis, Sphingomonas pituitosa and Burkholderia seminalis). The isolated strains were capable of producing indoleacetic acid (IAA) and organic acids that were able to reduce Al availability via a chelation process. These PSB isolates solubilized P (43.65%) existing in the growth media within 72 hours of incubation. Seedling of rice variety, MR 219, grown at pH 4, and with different concentrations of Al (0, 50 and 100 µM) was inoculated with these PSB strains. Results showed that the bacteria increased the pH with a concomitant reduction in Al concentration, which translated into better rice growth. The improved root volume and seedling dry weight of the inoculated plants indicated the potential of these isolates to be used in a bio-fertilizer formulation for rice cultivation on acid sulfate soils. Public Library of Science 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36643/1/36643.pdf Panhwar, Qurban Ali and Naher, Umme Aminun and Jusop, Shamsuddin and Othman, Radziah and Abdul Latif, Mohammad and Ismail, Mohd Razi (2014) Biochemical and molecular characterization of potential phosphate-solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth. PLOS ONE, 9 (10). art. no. e97241. pp. 1-14. ISSN 1932-6203 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0097241 10.1371/journal.pone.0097241 |
| spellingShingle | Panhwar, Qurban Ali Naher, Umme Aminun Jusop, Shamsuddin Othman, Radziah Abdul Latif, Mohammad Ismail, Mohd Razi Biochemical and molecular characterization of potential phosphate-solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth |
| title | Biochemical and molecular characterization of potential phosphate-solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth |
| title_full | Biochemical and molecular characterization of potential phosphate-solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth |
| title_fullStr | Biochemical and molecular characterization of potential phosphate-solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth |
| title_full_unstemmed | Biochemical and molecular characterization of potential phosphate-solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth |
| title_short | Biochemical and molecular characterization of potential phosphate-solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth |
| title_sort | biochemical and molecular characterization of potential phosphate solubilizing bacteria in acid sulfate soils and their beneficial effects on rice growth |
| url | http://psasir.upm.edu.my/id/eprint/36643/1/36643.pdf |
| work_keys_str_mv | AT panhwarqurbanali biochemicalandmolecularcharacterizationofpotentialphosphatesolubilizingbacteriainacidsulfatesoilsandtheirbeneficialeffectsonricegrowth AT naherummeaminun biochemicalandmolecularcharacterizationofpotentialphosphatesolubilizingbacteriainacidsulfatesoilsandtheirbeneficialeffectsonricegrowth AT jusopshamsuddin biochemicalandmolecularcharacterizationofpotentialphosphatesolubilizingbacteriainacidsulfatesoilsandtheirbeneficialeffectsonricegrowth AT othmanradziah biochemicalandmolecularcharacterizationofpotentialphosphatesolubilizingbacteriainacidsulfatesoilsandtheirbeneficialeffectsonricegrowth AT abdullatifmohammad biochemicalandmolecularcharacterizationofpotentialphosphatesolubilizingbacteriainacidsulfatesoilsandtheirbeneficialeffectsonricegrowth AT ismailmohdrazi biochemicalandmolecularcharacterizationofpotentialphosphatesolubilizingbacteriainacidsulfatesoilsandtheirbeneficialeffectsonricegrowth |