Characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube-based drug delivery system
The objective of the present study was to develop and characterize an in vitro sustained release formulation of silibinin (SB) using commercially available carboxylated multiwalled carbon nanotubes (COOH-MWCNTs) and to investigate cytotoxicity action of the synthesized nanohybrid (SB-MWCNTs). The re...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/36748/1/Characterization%20and%20in%20vitro%20sustained%20release%20of%20silibinin%20from%20ph%20responsive%20carbon%20nanotube.pdf |
| _version_ | 1825948902405177344 |
|---|---|
| author | Tan, Julia M. Karthivashan, Govindarajan Arulselvan, Palanisamy Fakurazi, Sharida Hussein, Mohd Zobir |
| author_facet | Tan, Julia M. Karthivashan, Govindarajan Arulselvan, Palanisamy Fakurazi, Sharida Hussein, Mohd Zobir |
| author_sort | Tan, Julia M. |
| collection | UPM |
| description | The objective of the present study was to develop and characterize an in vitro sustained release formulation of silibinin (SB) using commercially available carboxylated multiwalled carbon nanotubes (COOH-MWCNTs) and to investigate cytotoxicity action of the synthesized nanohybrid (SB-MWCNTs). The resulting nanohybrid was characterized with Fourier transform infrared (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), ultraviolet-visible spectrophotometry (UV-Vis), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). FTIR, Raman spectroscopy, and TGA analysis confirmed the adsorption of SB molecules to the COOH-MWCNTs. The release of SB from the COOH-MWCNTs nanocarrier was found to be sustained and pH-dependent. The maximum percentage release of SB from the nanocarrier reached approximately 96.6% and 43.1% within 1000 minutes when exposed to pH 7.4 and pH 4.8 solutions, respectively. It was observed that the release of kinetic behaviour of SB from the MWCNTs nanocarrier conformed well to pseudo-second order kinetic model. The obtained MTT result showed that the SB-MWCNTs exhibited enhanced cytotoxicity to human cancer cell lines in comparison with free SB at lower concentrations. These results suggest that SB-MWCNTs nanohybrid may be a promising nanodrug delivery system with sustained release property for the treatment of cancers. |
| first_indexed | 2024-03-06T08:36:15Z |
| format | Article |
| id | upm.eprints-36748 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:36:15Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-367482015-12-08T00:43:24Z http://psasir.upm.edu.my/id/eprint/36748/ Characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube-based drug delivery system Tan, Julia M. Karthivashan, Govindarajan Arulselvan, Palanisamy Fakurazi, Sharida Hussein, Mohd Zobir The objective of the present study was to develop and characterize an in vitro sustained release formulation of silibinin (SB) using commercially available carboxylated multiwalled carbon nanotubes (COOH-MWCNTs) and to investigate cytotoxicity action of the synthesized nanohybrid (SB-MWCNTs). The resulting nanohybrid was characterized with Fourier transform infrared (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), ultraviolet-visible spectrophotometry (UV-Vis), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). FTIR, Raman spectroscopy, and TGA analysis confirmed the adsorption of SB molecules to the COOH-MWCNTs. The release of SB from the COOH-MWCNTs nanocarrier was found to be sustained and pH-dependent. The maximum percentage release of SB from the nanocarrier reached approximately 96.6% and 43.1% within 1000 minutes when exposed to pH 7.4 and pH 4.8 solutions, respectively. It was observed that the release of kinetic behaviour of SB from the MWCNTs nanocarrier conformed well to pseudo-second order kinetic model. The obtained MTT result showed that the SB-MWCNTs exhibited enhanced cytotoxicity to human cancer cell lines in comparison with free SB at lower concentrations. These results suggest that SB-MWCNTs nanohybrid may be a promising nanodrug delivery system with sustained release property for the treatment of cancers. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36748/1/Characterization%20and%20in%20vitro%20sustained%20release%20of%20silibinin%20from%20ph%20responsive%20carbon%20nanotube.pdf Tan, Julia M. and Karthivashan, Govindarajan and Arulselvan, Palanisamy and Fakurazi, Sharida and Hussein, Mohd Zobir (2014) Characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube-based drug delivery system. Journal of Nanomaterials, 2014. art. no. 439873. pp. 1-10. ISSN 1687-4110; ESSN: 1687-4129 http://www.hindawi.com/journals/jnm/2014/439873/ 10.1155/2014/439873 |
| spellingShingle | Tan, Julia M. Karthivashan, Govindarajan Arulselvan, Palanisamy Fakurazi, Sharida Hussein, Mohd Zobir Characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube-based drug delivery system |
| title | Characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube-based drug delivery system |
| title_full | Characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube-based drug delivery system |
| title_fullStr | Characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube-based drug delivery system |
| title_full_unstemmed | Characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube-based drug delivery system |
| title_short | Characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube-based drug delivery system |
| title_sort | characterization and in vitro sustained release of silibinin from ph responsive carbon nanotube based drug delivery system |
| url | http://psasir.upm.edu.my/id/eprint/36748/1/Characterization%20and%20in%20vitro%20sustained%20release%20of%20silibinin%20from%20ph%20responsive%20carbon%20nanotube.pdf |
| work_keys_str_mv | AT tanjuliam characterizationandinvitrosustainedreleaseofsilibininfromphresponsivecarbonnanotubebaseddrugdeliverysystem AT karthivashangovindarajan characterizationandinvitrosustainedreleaseofsilibininfromphresponsivecarbonnanotubebaseddrugdeliverysystem AT arulselvanpalanisamy characterizationandinvitrosustainedreleaseofsilibininfromphresponsivecarbonnanotubebaseddrugdeliverysystem AT fakurazisharida characterizationandinvitrosustainedreleaseofsilibininfromphresponsivecarbonnanotubebaseddrugdeliverysystem AT husseinmohdzobir characterizationandinvitrosustainedreleaseofsilibininfromphresponsivecarbonnanotubebaseddrugdeliverysystem |