Characterisation of L. monocytogenes strains isolated from salad vegetables
Seventy-one strains of L. monocytogenes isolated from raw salad vegetables consumed in the minimally processed state in Malaysia were characterised by serotyping, random amplified polymorphic DNA-polymerase chain reaction analysis (RAPD-PCR) and antimicrobial susceptibility testing. Based on serotyp...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Inderscience Publishers
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36758/1/Characterisation%20of%20L.pdf |
| _version_ | 1825948904158396416 |
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| author | Ponniah, Jeyaletchumi Robin, Tunung Radu, Son Cheah, Yoke Kqueen Mohamad Ghazali, Farinazleen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep |
| author_facet | Ponniah, Jeyaletchumi Robin, Tunung Radu, Son Cheah, Yoke Kqueen Mohamad Ghazali, Farinazleen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep |
| author_sort | Ponniah, Jeyaletchumi |
| collection | UPM |
| description | Seventy-one strains of L. monocytogenes isolated from raw salad vegetables consumed in the minimally processed state in Malaysia were characterised by serotyping, random amplified polymorphic DNA-polymerase chain reaction analysis (RAPD-PCR) and antimicrobial susceptibility testing. Based on serotyping, only one strain belonged to serotype 4 while the rest were from serotype 1. RAPD-PCR typing was carried out using two primers, i.e., OPAR-8 and GEN 1-50-09. Based on RAPD-PCR fingerprinting, the strains could be grouped into six composite profile groups and a solitary isolate. The isolates were also evaluated against 16 antibiotics. Excluding nalidixic acid and oxacillin for which L. monocytogenes has natural resistance, the highest prevalence of resistance was against cephalexin. None of the isolates were resistant against imipenem and kanamycin. The multiple antibiotic resistance index (MARI) ranged from 0.06 to 0.63. It was found that most (82%) isolates were susceptible to antibiotics commonly used in veterinary and human listeriosis treatment. |
| first_indexed | 2024-03-06T08:36:16Z |
| format | Article |
| id | upm.eprints-36758 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:36:16Z |
| publishDate | 2015 |
| publisher | Inderscience Publishers |
| record_format | dspace |
| spelling | upm.eprints-367582015-10-28T02:38:46Z http://psasir.upm.edu.my/id/eprint/36758/ Characterisation of L. monocytogenes strains isolated from salad vegetables Ponniah, Jeyaletchumi Robin, Tunung Radu, Son Cheah, Yoke Kqueen Mohamad Ghazali, Farinazleen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep Seventy-one strains of L. monocytogenes isolated from raw salad vegetables consumed in the minimally processed state in Malaysia were characterised by serotyping, random amplified polymorphic DNA-polymerase chain reaction analysis (RAPD-PCR) and antimicrobial susceptibility testing. Based on serotyping, only one strain belonged to serotype 4 while the rest were from serotype 1. RAPD-PCR typing was carried out using two primers, i.e., OPAR-8 and GEN 1-50-09. Based on RAPD-PCR fingerprinting, the strains could be grouped into six composite profile groups and a solitary isolate. The isolates were also evaluated against 16 antibiotics. Excluding nalidixic acid and oxacillin for which L. monocytogenes has natural resistance, the highest prevalence of resistance was against cephalexin. None of the isolates were resistant against imipenem and kanamycin. The multiple antibiotic resistance index (MARI) ranged from 0.06 to 0.63. It was found that most (82%) isolates were susceptible to antibiotics commonly used in veterinary and human listeriosis treatment. Inderscience Publishers 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36758/1/Characterisation%20of%20L.pdf Ponniah, Jeyaletchumi and Robin, Tunung and Radu, Son and Cheah, Yoke Kqueen and Mohamad Ghazali, Farinazleen and Nishibuchi, Mitsuaki and Nakaguchi, Yoshitsugu and Malakar, Pradeep (2015) Characterisation of L. monocytogenes strains isolated from salad vegetables. International Journal of Food Safety, Nutrition and Public Health, 5 (2). pp. 137-150. ISSN 1479-3911; ESSN:1479-392X 10.1504/IJFSNPH.2015.067562 |
| spellingShingle | Ponniah, Jeyaletchumi Robin, Tunung Radu, Son Cheah, Yoke Kqueen Mohamad Ghazali, Farinazleen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep Characterisation of L. monocytogenes strains isolated from salad vegetables |
| title | Characterisation of L. monocytogenes strains isolated from salad vegetables |
| title_full | Characterisation of L. monocytogenes strains isolated from salad vegetables |
| title_fullStr | Characterisation of L. monocytogenes strains isolated from salad vegetables |
| title_full_unstemmed | Characterisation of L. monocytogenes strains isolated from salad vegetables |
| title_short | Characterisation of L. monocytogenes strains isolated from salad vegetables |
| title_sort | characterisation of l monocytogenes strains isolated from salad vegetables |
| url | http://psasir.upm.edu.my/id/eprint/36758/1/Characterisation%20of%20L.pdf |
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