Homing and engraftment of intravenously administered equine cord blood-derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses: a pilot project
Limb wounds on horses are often slow to heal and are prone to developing exuberant granulation tissue (EGT) and close primarily through epithelialization, which results in a cosmetically inferior and non-durable repair. In contrast, wounds on the body heal rapidly and primarily through contraction a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36793/1/36793.pdf |
| _version_ | 1796973013495709696 |
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| author | Mund, Suzanne J. K. Kawamura, Eiko Awang Junaidi, Awang Hazmi Campbell, John Wobeser, Bruce MacPhee, Daniel J. Honaramooz, Ali Barber, Spencer |
| author_facet | Mund, Suzanne J. K. Kawamura, Eiko Awang Junaidi, Awang Hazmi Campbell, John Wobeser, Bruce MacPhee, Daniel J. Honaramooz, Ali Barber, Spencer |
| author_sort | Mund, Suzanne J. K. |
| collection | UPM |
| description | Limb wounds on horses are often slow to heal and are prone to developing exuberant granulation tissue (EGT) and close primarily through epithelialization, which results in a cosmetically inferior and non-durable repair. In contrast, wounds on the body heal rapidly and primarily through contraction and rarely develop EGT. Intravenous (IV) multipotent mesenchymal stromal cells (MSCs) are promising. They home and engraft to cutaneous wounds and promote healing in laboratory animals, but this has not been demonstrated in horses. Furthermore, the clinical safety of administering >1.00 × 108 allogeneic MSCs IV to a horse has not been determined. A proof-of-principle pilot project was performed with two horses that were administered 1.02 × 108 fluorescently labeled allogeneic cord blood-derived MSCs (CB-MSCs) following wound creation on the forelimb and thorax. Wounds and contralateral non-wounded skin were sequentially biopsied on days 0, 1, 2, 7, 14, and 33 and evaluated with confocal microscopy to determine presence of homing and engraftment. Results confirmed preferential homing and engraftment to wounds with persistence of CB-MSCs at 33 days following wound creation, without clinically adverse reactions to the infusion. The absence of overt adverse reactions allows further studies to determine effects of IV CB-MSCs on equine wound healing. |
| first_indexed | 2024-03-06T08:36:23Z |
| format | Article |
| id | upm.eprints-36793 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:36:23Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-367932020-07-06T03:32:50Z http://psasir.upm.edu.my/id/eprint/36793/ Homing and engraftment of intravenously administered equine cord blood-derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses: a pilot project Mund, Suzanne J. K. Kawamura, Eiko Awang Junaidi, Awang Hazmi Campbell, John Wobeser, Bruce MacPhee, Daniel J. Honaramooz, Ali Barber, Spencer Limb wounds on horses are often slow to heal and are prone to developing exuberant granulation tissue (EGT) and close primarily through epithelialization, which results in a cosmetically inferior and non-durable repair. In contrast, wounds on the body heal rapidly and primarily through contraction and rarely develop EGT. Intravenous (IV) multipotent mesenchymal stromal cells (MSCs) are promising. They home and engraft to cutaneous wounds and promote healing in laboratory animals, but this has not been demonstrated in horses. Furthermore, the clinical safety of administering >1.00 × 108 allogeneic MSCs IV to a horse has not been determined. A proof-of-principle pilot project was performed with two horses that were administered 1.02 × 108 fluorescently labeled allogeneic cord blood-derived MSCs (CB-MSCs) following wound creation on the forelimb and thorax. Wounds and contralateral non-wounded skin were sequentially biopsied on days 0, 1, 2, 7, 14, and 33 and evaluated with confocal microscopy to determine presence of homing and engraftment. Results confirmed preferential homing and engraftment to wounds with persistence of CB-MSCs at 33 days following wound creation, without clinically adverse reactions to the infusion. The absence of overt adverse reactions allows further studies to determine effects of IV CB-MSCs on equine wound healing. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/36793/1/36793.pdf Mund, Suzanne J. K. and Kawamura, Eiko and Awang Junaidi, Awang Hazmi and Campbell, John and Wobeser, Bruce and MacPhee, Daniel J. and Honaramooz, Ali and Barber, Spencer (2020) Homing and engraftment of intravenously administered equine cord blood-derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses: a pilot project. Cells, 9 (5). art. no. 1162. pp. 1-23. ISSN 2073-4409 https://www.mdpi.com/2073-4409/9/5/1162 10.3390/cells9051162 |
| spellingShingle | Mund, Suzanne J. K. Kawamura, Eiko Awang Junaidi, Awang Hazmi Campbell, John Wobeser, Bruce MacPhee, Daniel J. Honaramooz, Ali Barber, Spencer Homing and engraftment of intravenously administered equine cord blood-derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses: a pilot project |
| title | Homing and engraftment of intravenously administered equine cord blood-derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses: a pilot project |
| title_full | Homing and engraftment of intravenously administered equine cord blood-derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses: a pilot project |
| title_fullStr | Homing and engraftment of intravenously administered equine cord blood-derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses: a pilot project |
| title_full_unstemmed | Homing and engraftment of intravenously administered equine cord blood-derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses: a pilot project |
| title_short | Homing and engraftment of intravenously administered equine cord blood-derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses: a pilot project |
| title_sort | homing and engraftment of intravenously administered equine cord blood derived multipotent mesenchymal stromal cells to surgically created cutaneous wound in horses a pilot project |
| url | http://psasir.upm.edu.my/id/eprint/36793/1/36793.pdf |
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