Physiochemical properties and specificity of mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link
The properties of mycelium-bound lipase of Aspergillus flavus Link obtained after 3 days growth in palm olein were studied. The mycelium-bound lipase has an optimum activity at a temperature range of 50–55°C and once extracted, the activity was optimum at a temperature range of 25–35 °C. The extract...
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| Format: | Article |
| Language: | English |
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Malaysian Agricultural Research and Development Institute (MARDI)
2000
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36849/1/Physiochemical%20properties%20and%20specificity%20of%20mycelium-bound%20lipase%20from%20a%20locally%20isolated%20strain%20of%20Aspergillus%20flavus%20Link.pdf |
| _version_ | 1796973024814039040 |
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| author | Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Bucke, Christopher |
| author_facet | Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Bucke, Christopher |
| author_sort | Long, Kamariah |
| collection | UPM |
| description | The properties of mycelium-bound lipase of Aspergillus flavus Link obtained after 3 days growth in palm olein were studied. The mycelium-bound lipase has an optimum activity at a temperature range of 50–55°C and once extracted, the activity was optimum at a temperature range of 25–35 °C. The extracted lipase was stable at alkaline pH and heat labile, thus lost 14% of its activity after being exposed to 30°C for 4 h. Ca2+ enhanced the lipase activity while EDTA (1 mM) had no effect. The enzyme hydrolysed coconut oil faster than other vegetable oils and tributyrin was not hydrolysed by the extracted lipase. The apparent Km values obtained for mycelium-bound lipase (11.76 mg/mL) was three times higher than the extracted lipase (3.92 mg/mL) suggesting that there was a diffusion limitation of the substrate in reaching the bound lipase. The extracted lipase displayed 1,3 positional specificity. |
| first_indexed | 2024-03-06T08:36:33Z |
| format | Article |
| id | upm.eprints-36849 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:36:33Z |
| publishDate | 2000 |
| publisher | Malaysian Agricultural Research and Development Institute (MARDI) |
| record_format | dspace |
| spelling | upm.eprints-368492016-05-17T06:56:14Z http://psasir.upm.edu.my/id/eprint/36849/ Physiochemical properties and specificity of mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Bucke, Christopher The properties of mycelium-bound lipase of Aspergillus flavus Link obtained after 3 days growth in palm olein were studied. The mycelium-bound lipase has an optimum activity at a temperature range of 50–55°C and once extracted, the activity was optimum at a temperature range of 25–35 °C. The extracted lipase was stable at alkaline pH and heat labile, thus lost 14% of its activity after being exposed to 30°C for 4 h. Ca2+ enhanced the lipase activity while EDTA (1 mM) had no effect. The enzyme hydrolysed coconut oil faster than other vegetable oils and tributyrin was not hydrolysed by the extracted lipase. The apparent Km values obtained for mycelium-bound lipase (11.76 mg/mL) was three times higher than the extracted lipase (3.92 mg/mL) suggesting that there was a diffusion limitation of the substrate in reaching the bound lipase. The extracted lipase displayed 1,3 positional specificity. Malaysian Agricultural Research and Development Institute (MARDI) 2000 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36849/1/Physiochemical%20properties%20and%20specificity%20of%20mycelium-bound%20lipase%20from%20a%20locally%20isolated%20strain%20of%20Aspergillus%20flavus%20Link.pdf Long, Kamariah and Mohd Ghazali, Hasanah and Ariff, Arbakariya and Bucke, Christopher (2000) Physiochemical properties and specificity of mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link. Journal of Tropical Agriculture and Food Science, 28 (2). pp. 135-142. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/87-2000/volume-28-no2/593-280210 |
| spellingShingle | Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Bucke, Christopher Physiochemical properties and specificity of mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link |
| title | Physiochemical properties and specificity of mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link |
| title_full | Physiochemical properties and specificity of mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link |
| title_fullStr | Physiochemical properties and specificity of mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link |
| title_full_unstemmed | Physiochemical properties and specificity of mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link |
| title_short | Physiochemical properties and specificity of mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link |
| title_sort | physiochemical properties and specificity of mycelium bound lipase from a locally isolated strain of aspergillus flavus link |
| url | http://psasir.upm.edu.my/id/eprint/36849/1/Physiochemical%20properties%20and%20specificity%20of%20mycelium-bound%20lipase%20from%20a%20locally%20isolated%20strain%20of%20Aspergillus%20flavus%20Link.pdf |
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