Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21
A vector that drives the expression of the reporter gusA gene in both Lactobacillus plantarum and Lactococcus lactis was constructed in this study. This vector contained a newly characterized heat shock promoter (Phsp), amplified from an Enterococcus faecium plasmid, pAR6. Functionality and characte...
| Main Authors: | , , , , , |
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| Format: | Article |
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Elsevier
2014
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| _version_ | 1825948930327707648 |
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| author | Maidin, Mohd Shawal Thakib Song, Adelene Ai Lian Jalilsood, Tannaz Sieo, Chin Chin Yusoff, Khatijah Abdul Rahim, Raha |
| author_facet | Maidin, Mohd Shawal Thakib Song, Adelene Ai Lian Jalilsood, Tannaz Sieo, Chin Chin Yusoff, Khatijah Abdul Rahim, Raha |
| author_sort | Maidin, Mohd Shawal Thakib |
| collection | UPM |
| description | A vector that drives the expression of the reporter gusA gene in both Lactobacillus plantarum and Lactococcus lactis was constructed in this study. This vector contained a newly characterized heat shock promoter (Phsp), amplified from an Enterococcus faecium plasmid, pAR6. Functionality and characterization of this promoter was initially performed by cloning Phsp into pNZ8008, a commercial lactococcal plasmid used for screening of putative promoters which utilizes gusA as a reporter. It was observed that Phsp was induced under heat, salinity and alkaline stresses or a combination of all three stresses. The newly characterized Phsp promoter was then used to construct a novel Lactobacillus vector, pAR1801 and its ability to express the gusA under stress-induced conditions was reproducible in both Lb. plantarum Pa21 and L. lactis M4 hosts. |
| first_indexed | 2024-03-06T08:36:41Z |
| format | Article |
| id | upm.eprints-36892 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:36:41Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-368922016-01-12T00:26:36Z http://psasir.upm.edu.my/id/eprint/36892/ Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21 Maidin, Mohd Shawal Thakib Song, Adelene Ai Lian Jalilsood, Tannaz Sieo, Chin Chin Yusoff, Khatijah Abdul Rahim, Raha A vector that drives the expression of the reporter gusA gene in both Lactobacillus plantarum and Lactococcus lactis was constructed in this study. This vector contained a newly characterized heat shock promoter (Phsp), amplified from an Enterococcus faecium plasmid, pAR6. Functionality and characterization of this promoter was initially performed by cloning Phsp into pNZ8008, a commercial lactococcal plasmid used for screening of putative promoters which utilizes gusA as a reporter. It was observed that Phsp was induced under heat, salinity and alkaline stresses or a combination of all three stresses. The newly characterized Phsp promoter was then used to construct a novel Lactobacillus vector, pAR1801 and its ability to express the gusA under stress-induced conditions was reproducible in both Lb. plantarum Pa21 and L. lactis M4 hosts. Elsevier 2014-07 Article PeerReviewed Maidin, Mohd Shawal Thakib and Song, Adelene Ai Lian and Jalilsood, Tannaz and Sieo, Chin Chin and Yusoff, Khatijah and Abdul Rahim, Raha (2014) Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21. Plasmid, 74. pp. 32-38. ISSN 0147-619X; ESSN:1095-9890 http://www.sciencedirect.com/science/article/pii/S0147619X14000298 10.1016/j.plasmid.2014.05.003 |
| spellingShingle | Maidin, Mohd Shawal Thakib Song, Adelene Ai Lian Jalilsood, Tannaz Sieo, Chin Chin Yusoff, Khatijah Abdul Rahim, Raha Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21 |
| title | Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21 |
| title_full | Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21 |
| title_fullStr | Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21 |
| title_full_unstemmed | Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21 |
| title_short | Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21 |
| title_sort | construction of a novel inducible expression vector for lactococcus lactis m4 and lactobacillus plantarum pa21 |
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