Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia
Yellowing symptoms similar to coconut yellow decline phytoplasma disease were observed on lipstick palms (Cyrtostachys renda) in Selangor state, Malaysia. Typical symptoms were yellowing, light green fronds, gradual collapse of older fronds and decline in growth. Polymerase chain reaction assay was...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Blackwell Verlag GmbH
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37007/1/Detection%20and%20identification%20of%20aster%20yellows%20phytoplasma%20associated%20with%20lipstick%20yellow%20frond%20disease%20in%20Malaysia.pdf |
| _version_ | 1825948952741019648 |
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| author | Naderali, Neda Vadamalai, Ganesan Tan, Yee How Nejat, Naghmeh |
| author_facet | Naderali, Neda Vadamalai, Ganesan Tan, Yee How Nejat, Naghmeh |
| author_sort | Naderali, Neda |
| collection | UPM |
| description | Yellowing symptoms similar to coconut yellow decline phytoplasma disease were observed on lipstick palms (Cyrtostachys renda) in Selangor state, Malaysia. Typical symptoms were yellowing, light green fronds, gradual collapse of older fronds and decline in growth. Polymerase chain reaction assay was employed to detect phytoplasma in symptomatic lipstick palms. Extracted DNA was amplified from symptomatic lipstick palms by PCR using phytoplasma-universal primer pair P1/P7 followed by R16F2n/R16R2. Phytoplasma presence was confirmed, and the 1250 bp products were cloned and sequenced. Sequence analysis indicated that the phytoplasmas associated with lipstick yellow frond disease were isolates of ‘Candidatus Phytoplasma asteris’ belonging to the 16SrI group. Virtual RFLP analysis of the resulting profiles revealed that these palm-infecting phytoplasmas belong to subgroup 16SrI-B and a possibly new 16SrI-subgroup. This is the first report of lipstick palm as a new host of aster yellows phytoplasma (16SrI) in Malaysia and worldwide. |
| first_indexed | 2024-03-06T08:37:01Z |
| format | Article |
| id | upm.eprints-37007 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:37:01Z |
| publishDate | 2014 |
| publisher | Blackwell Verlag GmbH |
| record_format | dspace |
| spelling | upm.eprints-370072015-12-03T06:51:09Z http://psasir.upm.edu.my/id/eprint/37007/ Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia Naderali, Neda Vadamalai, Ganesan Tan, Yee How Nejat, Naghmeh Yellowing symptoms similar to coconut yellow decline phytoplasma disease were observed on lipstick palms (Cyrtostachys renda) in Selangor state, Malaysia. Typical symptoms were yellowing, light green fronds, gradual collapse of older fronds and decline in growth. Polymerase chain reaction assay was employed to detect phytoplasma in symptomatic lipstick palms. Extracted DNA was amplified from symptomatic lipstick palms by PCR using phytoplasma-universal primer pair P1/P7 followed by R16F2n/R16R2. Phytoplasma presence was confirmed, and the 1250 bp products were cloned and sequenced. Sequence analysis indicated that the phytoplasmas associated with lipstick yellow frond disease were isolates of ‘Candidatus Phytoplasma asteris’ belonging to the 16SrI group. Virtual RFLP analysis of the resulting profiles revealed that these palm-infecting phytoplasmas belong to subgroup 16SrI-B and a possibly new 16SrI-subgroup. This is the first report of lipstick palm as a new host of aster yellows phytoplasma (16SrI) in Malaysia and worldwide. Blackwell Verlag GmbH 2014-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37007/1/Detection%20and%20identification%20of%20aster%20yellows%20phytoplasma%20associated%20with%20lipstick%20yellow%20frond%20disease%20in%20Malaysia.pdf Naderali, Neda and Vadamalai, Ganesan and Tan, Yee How and Nejat, Naghmeh (2014) Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia. Journal of Phytopathology, 162 (4). pp. 264-268. ISSN 0931-1785 10.1111/jph.12181 |
| spellingShingle | Naderali, Neda Vadamalai, Ganesan Tan, Yee How Nejat, Naghmeh Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia |
| title | Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia |
| title_full | Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia |
| title_fullStr | Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia |
| title_full_unstemmed | Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia |
| title_short | Detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in Malaysia |
| title_sort | detection and identification of aster yellows phytoplasma associated with lipstick yellow frond disease in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/37007/1/Detection%20and%20identification%20of%20aster%20yellows%20phytoplasma%20associated%20with%20lipstick%20yellow%20frond%20disease%20in%20Malaysia.pdf |
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