Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals
An inhibitive assay for metals using a bacterial respiratory assay system is presented. The assay is based on the ability of bacteria to reduce the water soluble tetrazolium dye (MTT). The isolate was tentatively identified as Bacillus sp. strain Khayat. The Bacillus sp based MTT assay is sensitive...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Friends Science Publishers
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37052/1/Development%20of%20a%20bacterial.pdf |
| _version_ | 1825948961350877184 |
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| author | Wasoh @ Mohamad Isa, Helmi Wan Johari, Wan Lutfi Syahir, A. Abd. Shukor, Mohd Yunus Abd Aziz, Nor Azwady Shaharuddin, N. A. Mustafa, Muskhazli |
| author_facet | Wasoh @ Mohamad Isa, Helmi Wan Johari, Wan Lutfi Syahir, A. Abd. Shukor, Mohd Yunus Abd Aziz, Nor Azwady Shaharuddin, N. A. Mustafa, Muskhazli |
| author_sort | Wasoh @ Mohamad Isa, Helmi |
| collection | UPM |
| description | An inhibitive assay for metals using a bacterial respiratory assay system is presented. The assay is based on the ability of bacteria to reduce the water soluble tetrazolium dye (MTT). The isolate was tentatively identified as Bacillus sp. strain Khayat. The Bacillus sp based MTT assay is sensitive towards Hg2+, Cu2+, Ag2+, Cd2+ and Zn2+ with concentration of toxicant giving 50% inhibition (IC50) values at 0.046, 0.057, 0.044, 0.857 and 1.716 mg/L, respectively. A Limit of Detection (LOD) value was 0.001 mg/L for Hg2+ and Cu2+ while 0.003, 0.067 and 0.201 mg/L, respectively for Ag2+, Cd2+ and Zn2+. This assay is xenobiotics and pesticide tolerance and can be completed within 20 min. Field test on identify polluted water sample from Bukit Tinggi Industrial Estate, Penang and Bukit Tinggi Industrial Estate, Penang proved that Bacillus sp-based MTT assay was sensitive in toxic response. |
| first_indexed | 2024-03-06T08:37:09Z |
| format | Article |
| id | upm.eprints-37052 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:37:09Z |
| publishDate | 2014 |
| publisher | Friends Science Publishers |
| record_format | dspace |
| spelling | upm.eprints-370522015-10-09T00:28:04Z http://psasir.upm.edu.my/id/eprint/37052/ Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals Wasoh @ Mohamad Isa, Helmi Wan Johari, Wan Lutfi Syahir, A. Abd. Shukor, Mohd Yunus Abd Aziz, Nor Azwady Shaharuddin, N. A. Mustafa, Muskhazli An inhibitive assay for metals using a bacterial respiratory assay system is presented. The assay is based on the ability of bacteria to reduce the water soluble tetrazolium dye (MTT). The isolate was tentatively identified as Bacillus sp. strain Khayat. The Bacillus sp based MTT assay is sensitive towards Hg2+, Cu2+, Ag2+, Cd2+ and Zn2+ with concentration of toxicant giving 50% inhibition (IC50) values at 0.046, 0.057, 0.044, 0.857 and 1.716 mg/L, respectively. A Limit of Detection (LOD) value was 0.001 mg/L for Hg2+ and Cu2+ while 0.003, 0.067 and 0.201 mg/L, respectively for Ag2+, Cd2+ and Zn2+. This assay is xenobiotics and pesticide tolerance and can be completed within 20 min. Field test on identify polluted water sample from Bukit Tinggi Industrial Estate, Penang and Bukit Tinggi Industrial Estate, Penang proved that Bacillus sp-based MTT assay was sensitive in toxic response. Friends Science Publishers 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37052/1/Development%20of%20a%20bacterial.pdf Wasoh @ Mohamad Isa, Helmi and Wan Johari, Wan Lutfi and Syahir, A. and Abd. Shukor, Mohd Yunus and Abd Aziz, Nor Azwady and Shaharuddin, N. A. and Mustafa, Muskhazli (2014) Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals. International Journal of Agriculture and Biology, 16 (6). 1123 - 1128. ISSN 1560-8530; ESSN: 1814–9596 http://www.fspublishers.org/Issue.php?categoryID=127 |
| spellingShingle | Wasoh @ Mohamad Isa, Helmi Wan Johari, Wan Lutfi Syahir, A. Abd. Shukor, Mohd Yunus Abd Aziz, Nor Azwady Shaharuddin, N. A. Mustafa, Muskhazli Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals |
| title | Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals |
| title_full | Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals |
| title_fullStr | Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals |
| title_full_unstemmed | Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals |
| title_short | Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals |
| title_sort | development of a bacterial based tetrazolium dye mtt assay for monitoring of heavy metals |
| url | http://psasir.upm.edu.my/id/eprint/37052/1/Development%20of%20a%20bacterial.pdf |
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