Differentially expressed proteins in ER+ MCF7 and ER- MDA- MB-231 human breast cancer cells by RhoGDI-α silencing and overexpression
Background: The consequence of Rho GDP dissociation inhibitor alpha (RhoGDIα) activity on migration and invasion of estrogen receptor positive (ER+) and negative (ER-) breast cancer cells has not been studied using the proteomic approach. Changes in expression of RhoGDIα and other proteins interac...
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| 格式: | 文件 |
| 语言: | English |
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Asian Pacific Organization for Cancer Prevention
2014
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| 在线阅读: | http://psasir.upm.edu.my/id/eprint/37106/1/Differentially%20expressed%20proteins%20in%20ER.pdf |
| _version_ | 1825948971863900160 |
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| author | Hooshmand, Somayeh Ghaderi, Abbas Yusoff, Khatijah Karuppiah, Thilakavathy Rosli, Rozita Mojtahedi, Zahra |
| author_facet | Hooshmand, Somayeh Ghaderi, Abbas Yusoff, Khatijah Karuppiah, Thilakavathy Rosli, Rozita Mojtahedi, Zahra |
| author_sort | Hooshmand, Somayeh |
| collection | UPM |
| description | Background: The consequence of Rho GDP dissociation inhibitor alpha (RhoGDIα) activity on migration and
invasion of estrogen receptor positive (ER+) and negative (ER-) breast cancer cells has not been studied using the
proteomic approach. Changes in expression of RhoGDIα and other proteins interacting directly or indirectly with
RhoGDIα in MCF7 and MDA-MB-231, with different metastatic potentials is of particular interest. Materials
and Methods: ER+ MCF7 and ER- MDA-MB-231 cell lines were subjected to two-dimensional electrophoresis
(2-DE) and spots of interest were identified by matrix-assisted laser desorption/ionization time of- flight/timeof-
flight (MALDI-TOF/TOF) mass spectrometry (MS) analysis after downregulation of RhoGDIα using short
interfering RNA (siRNA) and upregulated using GFP-tagged ORF clone of RhoGDIα. Results: The results
showed a total of 35 proteins that were either up- or down-regulated in these cells. Here we identifed 9 and 15
proteins differentially expressed with silencing of RhoGDIα in MCF-7 and the MDA-MB-231 cells, respectively.
In addition, 10 proteins were differentially expressed in the upregulation of RhoGDIα in MCF7, while only one
protein was identified in the upregulation of RhoGDIα in MDA-MB-231. Based on the biological functions of these
proteins, the results revealed that proteins involved in cell migration are more strongly altered with RhoGDI-α
activity. Although several of these proteins have been previously indicated in tumorigenesis and invasiveness of
breast cancer cells, some ohave not been previously reported to be involved in breast cancer migration. Hence,
these proteins may serve as useful candidate biomarkers for tumorigenesis and invasiveness of breast cancer
cells. Conclusions: Future studies are needed to determine the mechanisms by which these proteins regulate cell
migration. The combination of RhoGDIα with other potential biomarkers may be a more promising approach
in the inhibition of breast cancer cell migration. |
| first_indexed | 2024-03-06T08:37:19Z |
| format | Article |
| id | upm.eprints-37106 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:37:19Z |
| publishDate | 2014 |
| publisher | Asian Pacific Organization for Cancer Prevention |
| record_format | dspace |
| spelling | upm.eprints-371062015-09-10T06:27:01Z http://psasir.upm.edu.my/id/eprint/37106/ Differentially expressed proteins in ER+ MCF7 and ER- MDA- MB-231 human breast cancer cells by RhoGDI-α silencing and overexpression Hooshmand, Somayeh Ghaderi, Abbas Yusoff, Khatijah Karuppiah, Thilakavathy Rosli, Rozita Mojtahedi, Zahra Background: The consequence of Rho GDP dissociation inhibitor alpha (RhoGDIα) activity on migration and invasion of estrogen receptor positive (ER+) and negative (ER-) breast cancer cells has not been studied using the proteomic approach. Changes in expression of RhoGDIα and other proteins interacting directly or indirectly with RhoGDIα in MCF7 and MDA-MB-231, with different metastatic potentials is of particular interest. Materials and Methods: ER+ MCF7 and ER- MDA-MB-231 cell lines were subjected to two-dimensional electrophoresis (2-DE) and spots of interest were identified by matrix-assisted laser desorption/ionization time of- flight/timeof- flight (MALDI-TOF/TOF) mass spectrometry (MS) analysis after downregulation of RhoGDIα using short interfering RNA (siRNA) and upregulated using GFP-tagged ORF clone of RhoGDIα. Results: The results showed a total of 35 proteins that were either up- or down-regulated in these cells. Here we identifed 9 and 15 proteins differentially expressed with silencing of RhoGDIα in MCF-7 and the MDA-MB-231 cells, respectively. In addition, 10 proteins were differentially expressed in the upregulation of RhoGDIα in MCF7, while only one protein was identified in the upregulation of RhoGDIα in MDA-MB-231. Based on the biological functions of these proteins, the results revealed that proteins involved in cell migration are more strongly altered with RhoGDI-α activity. Although several of these proteins have been previously indicated in tumorigenesis and invasiveness of breast cancer cells, some ohave not been previously reported to be involved in breast cancer migration. Hence, these proteins may serve as useful candidate biomarkers for tumorigenesis and invasiveness of breast cancer cells. Conclusions: Future studies are needed to determine the mechanisms by which these proteins regulate cell migration. The combination of RhoGDIα with other potential biomarkers may be a more promising approach in the inhibition of breast cancer cell migration. Asian Pacific Organization for Cancer Prevention 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37106/1/Differentially%20expressed%20proteins%20in%20ER.pdf Hooshmand, Somayeh and Ghaderi, Abbas and Yusoff, Khatijah and Karuppiah, Thilakavathy and Rosli, Rozita and Mojtahedi, Zahra (2014) Differentially expressed proteins in ER+ MCF7 and ER- MDA- MB-231 human breast cancer cells by RhoGDI-α silencing and overexpression. Asian Pacific Journal of Cancer Prevention, 15 (7). pp. 3311-3317. ISSN 1513-7368 http://www.apocpcontrol.org/page/apjcp_issues_view.php?sid=Entrez:PubMed&id=pmid:24815488&key=2014.15.7.3311 10.7314/APJCP.2014.15.7.3311 |
| spellingShingle | Hooshmand, Somayeh Ghaderi, Abbas Yusoff, Khatijah Karuppiah, Thilakavathy Rosli, Rozita Mojtahedi, Zahra Differentially expressed proteins in ER+ MCF7 and ER- MDA- MB-231 human breast cancer cells by RhoGDI-α silencing and overexpression |
| title | Differentially expressed proteins in ER+ MCF7 and ER- MDA- MB-231 human breast cancer cells by RhoGDI-α silencing and overexpression |
| title_full | Differentially expressed proteins in ER+ MCF7 and ER- MDA- MB-231 human breast cancer cells by RhoGDI-α silencing and overexpression |
| title_fullStr | Differentially expressed proteins in ER+ MCF7 and ER- MDA- MB-231 human breast cancer cells by RhoGDI-α silencing and overexpression |
| title_full_unstemmed | Differentially expressed proteins in ER+ MCF7 and ER- MDA- MB-231 human breast cancer cells by RhoGDI-α silencing and overexpression |
| title_short | Differentially expressed proteins in ER+ MCF7 and ER- MDA- MB-231 human breast cancer cells by RhoGDI-α silencing and overexpression |
| title_sort | differentially expressed proteins in er mcf7 and er mda mb 231 human breast cancer cells by rhogdi α silencing and overexpression |
| url | http://psasir.upm.edu.my/id/eprint/37106/1/Differentially%20expressed%20proteins%20in%20ER.pdf |
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