Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch
Direct assay of resistant starch (RS) in food and feed is accomplished by (i) removal of lipid, protein, and digestible starch to obtain insoluble dietary fiber, and (ii) dissolution of the resistant starch in the insoluble fiber followed by its quantification with specific enzymes. Phosphorylated c...
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| Format: | Article |
| Language: | English English |
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Elsevier
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37112/1/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf http://psasir.upm.edu.my/id/eprint/37112/2/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf |
| _version_ | 1796973078809411584 |
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| author | Shukri, Radhiah Zhu, Lijia Seib, Paul A. Maningat, Clodualdo Shi, Yong Cheng |
| author_facet | Shukri, Radhiah Zhu, Lijia Seib, Paul A. Maningat, Clodualdo Shi, Yong Cheng |
| author_sort | Shukri, Radhiah |
| collection | UPM |
| description | Direct assay of resistant starch (RS) in food and feed is accomplished by (i) removal of lipid, protein, and digestible starch to obtain insoluble dietary fiber, and (ii) dissolution of the resistant starch in the insoluble fiber followed by its quantification with specific enzymes. Phosphorylated cross-linked (CL) RS resists dissolution and therefore has not been assayed directly. The objective of this study was to develop a method to solubilize the RS fraction in phosphorylated (0.4% phosphorus) CL wheat starch (RS4) after its incubation with α-amylase and amyloglucosidase for 16 h at 37 °C as directed by the RS assay AOAC Method 2002.02. The residue was hydrolyzed and solubilized by conducting two back-to-back incubations with thermostable α-amylase for 30 min at 100 °C and pH 5.0, cooling to 50 °C, then incubating quickly with amyloglucosidase at 50 °C for 1 h at pH 5.0. Importantly, the cooling process after α-amylase incubation was done by placing the mixture in a water bath at 50 °C. The degree of hydrolysis of the CL phosphorylated wheat starch was determined as d-glucose using high-performance anion-exchange chromatography with pulsed amperometric detection (99.0%), glucose-oxidase/peroxidase (95.3%), and phenol-sulfuric acid determination of total carbohydrate (105.2%). Based on those findings, we propose a direct determination of RS in foods containing phosphorylated CL wheat starch. |
| first_indexed | 2024-03-06T08:37:20Z |
| format | Article |
| id | upm.eprints-37112 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:37:20Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-371122020-04-15T16:52:07Z http://psasir.upm.edu.my/id/eprint/37112/ Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch Shukri, Radhiah Zhu, Lijia Seib, Paul A. Maningat, Clodualdo Shi, Yong Cheng Direct assay of resistant starch (RS) in food and feed is accomplished by (i) removal of lipid, protein, and digestible starch to obtain insoluble dietary fiber, and (ii) dissolution of the resistant starch in the insoluble fiber followed by its quantification with specific enzymes. Phosphorylated cross-linked (CL) RS resists dissolution and therefore has not been assayed directly. The objective of this study was to develop a method to solubilize the RS fraction in phosphorylated (0.4% phosphorus) CL wheat starch (RS4) after its incubation with α-amylase and amyloglucosidase for 16 h at 37 °C as directed by the RS assay AOAC Method 2002.02. The residue was hydrolyzed and solubilized by conducting two back-to-back incubations with thermostable α-amylase for 30 min at 100 °C and pH 5.0, cooling to 50 °C, then incubating quickly with amyloglucosidase at 50 °C for 1 h at pH 5.0. Importantly, the cooling process after α-amylase incubation was done by placing the mixture in a water bath at 50 °C. The degree of hydrolysis of the CL phosphorylated wheat starch was determined as d-glucose using high-performance anion-exchange chromatography with pulsed amperometric detection (99.0%), glucose-oxidase/peroxidase (95.3%), and phenol-sulfuric acid determination of total carbohydrate (105.2%). Based on those findings, we propose a direct determination of RS in foods containing phosphorylated CL wheat starch. Elsevier 2015-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37112/1/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/37112/2/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf Shukri, Radhiah and Zhu, Lijia and Seib, Paul A. and Maningat, Clodualdo and Shi, Yong Cheng (2015) Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch. Bioactive Carbohydrates and Dietary Fibre, 5 (1). pp. 1-9. ISSN 2212-6198 http://www.sciencedirect.com/science/article/pii/S2212619814000539 10.1016/j.bcdf.2014.11.001 |
| spellingShingle | Shukri, Radhiah Zhu, Lijia Seib, Paul A. Maningat, Clodualdo Shi, Yong Cheng Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch |
| title | Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch |
| title_full | Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch |
| title_fullStr | Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch |
| title_full_unstemmed | Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch |
| title_short | Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch |
| title_sort | direct in vitro assay of resistant starch in phosphorylated cross linked starch |
| url | http://psasir.upm.edu.my/id/eprint/37112/1/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf http://psasir.upm.edu.my/id/eprint/37112/2/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf |
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