Early detection of biofilm formation of selected bacterial isolates through a new screening method using 'image J'
Aim: The cells growing in biofilms are physiologically distinct even from the same microorganism in the planktonic state. Although mixed-species biofilms predominate in most of the environments, single-species biofilms exist in a large variety of infections, on the surface of medical implants and pa...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Microbiology
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37182/1/Early%20detection%20of%20biofilm%20formation%20of%20selected%20bacterial%20isolates%20through%20a%20new%20screening%20method%20using.pdf |
| _version_ | 1796973095042416640 |
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| author | Shumi, Wahhida Chowdhury, Rejaul Haque Mostafa Hossain, Md. Aktar Foo, Hooi Ling |
| author_facet | Shumi, Wahhida Chowdhury, Rejaul Haque Mostafa Hossain, Md. Aktar Foo, Hooi Ling |
| author_sort | Shumi, Wahhida |
| collection | UPM |
| description | Aim: The cells growing in biofilms are physiologically distinct even from the same microorganism in the planktonic state. Although mixed-species biofilms predominate in most of the environments, single-species biofilms exist in a large variety of infections, on the surface of medical implants and parts of the machineries in the food industries. Convenient method and early detection of these biofilms are therefore, necessary to avoid or prevent the important implants from the bacterial biofilm formation. Therefore present research was undertaken with the aim to establish an in vitro method for early detection and screening of biofilm with easily applicable image J. Methodology and results: Eight bacterial colonies were isolated from the poultry wastes (PW), dental plaques (DP) and hospital effluents (HE). Among them, three isolates were finally selected for the study based on their source of origin, colony characteristics and the biofilm forming ability determined by test tube assay. The isolates were provisionally identified as Salmonella arizonae (from PW), Micrococcus luteus (from DP) and Aerococcus viridians (from HE) and examined for their biofilm forming ability through a novel in vitro method, cover slip assay. The images of crystal violet stained biofilms of the isolates on the surface of the cover slips were captured and analysed by image-processing software image J. The results of the cover slip assay were then compared with those from test tube assays to conform the efficacy and reliability of the method for screening and evaluating biofilm formation. The results suggest that the novel in vitro method for biofilm screening by cover slip assay is effective for evaluating selected bacterial biofilms. The ability to form biofilm was not specifically correlated with the colony characteristics but the initial attachment for early development of the biofilm was significantly correlated with their motility. Conclusion, significance and impact of study: The method of biofilm screening with the cover slip assay used in this study is novel, very simple but powerful and effective method, is expected to have significant impact and gain additional interest among the scientists for biofilm screening and study. |
| first_indexed | 2024-03-06T08:37:32Z |
| format | Article |
| id | upm.eprints-37182 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:37:32Z |
| publishDate | 2014 |
| publisher | Malaysian Society for Microbiology |
| record_format | dspace |
| spelling | upm.eprints-371822016-01-27T01:20:00Z http://psasir.upm.edu.my/id/eprint/37182/ Early detection of biofilm formation of selected bacterial isolates through a new screening method using 'image J' Shumi, Wahhida Chowdhury, Rejaul Haque Mostafa Hossain, Md. Aktar Foo, Hooi Ling Aim: The cells growing in biofilms are physiologically distinct even from the same microorganism in the planktonic state. Although mixed-species biofilms predominate in most of the environments, single-species biofilms exist in a large variety of infections, on the surface of medical implants and parts of the machineries in the food industries. Convenient method and early detection of these biofilms are therefore, necessary to avoid or prevent the important implants from the bacterial biofilm formation. Therefore present research was undertaken with the aim to establish an in vitro method for early detection and screening of biofilm with easily applicable image J. Methodology and results: Eight bacterial colonies were isolated from the poultry wastes (PW), dental plaques (DP) and hospital effluents (HE). Among them, three isolates were finally selected for the study based on their source of origin, colony characteristics and the biofilm forming ability determined by test tube assay. The isolates were provisionally identified as Salmonella arizonae (from PW), Micrococcus luteus (from DP) and Aerococcus viridians (from HE) and examined for their biofilm forming ability through a novel in vitro method, cover slip assay. The images of crystal violet stained biofilms of the isolates on the surface of the cover slips were captured and analysed by image-processing software image J. The results of the cover slip assay were then compared with those from test tube assays to conform the efficacy and reliability of the method for screening and evaluating biofilm formation. The results suggest that the novel in vitro method for biofilm screening by cover slip assay is effective for evaluating selected bacterial biofilms. The ability to form biofilm was not specifically correlated with the colony characteristics but the initial attachment for early development of the biofilm was significantly correlated with their motility. Conclusion, significance and impact of study: The method of biofilm screening with the cover slip assay used in this study is novel, very simple but powerful and effective method, is expected to have significant impact and gain additional interest among the scientists for biofilm screening and study. Malaysian Society for Microbiology 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37182/1/Early%20detection%20of%20biofilm%20formation%20of%20selected%20bacterial%20isolates%20through%20a%20new%20screening%20method%20using.pdf Shumi, Wahhida and Chowdhury, Rejaul Haque Mostafa and Hossain, Md. Aktar and Foo, Hooi Ling (2014) Early detection of biofilm formation of selected bacterial isolates through a new screening method using 'image J'. Malaysian Journal of Microbiology, 10 (3). pp. 179-185. ISSN 1823-8262; ESSN: 2231-7538 http://web.usm.my/mjm/issues/vol10no3/Research%205.pdf |
| spellingShingle | Shumi, Wahhida Chowdhury, Rejaul Haque Mostafa Hossain, Md. Aktar Foo, Hooi Ling Early detection of biofilm formation of selected bacterial isolates through a new screening method using 'image J' |
| title | Early detection of biofilm formation of selected bacterial isolates through a new screening method using 'image J' |
| title_full | Early detection of biofilm formation of selected bacterial isolates through a new screening method using 'image J' |
| title_fullStr | Early detection of biofilm formation of selected bacterial isolates through a new screening method using 'image J' |
| title_full_unstemmed | Early detection of biofilm formation of selected bacterial isolates through a new screening method using 'image J' |
| title_short | Early detection of biofilm formation of selected bacterial isolates through a new screening method using 'image J' |
| title_sort | early detection of biofilm formation of selected bacterial isolates through a new screening method using image j |
| url | http://psasir.upm.edu.my/id/eprint/37182/1/Early%20detection%20of%20biofilm%20formation%20of%20selected%20bacterial%20isolates%20through%20a%20new%20screening%20method%20using.pdf |
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