Effectiveness of Haemagglutination test in detection of Newcastle disease virus (NDV) AF2240 strain from harvested allantoic fluids of infected chicken eggs
Haemagglutination (HA) test for the detection of Newcastle disease virus (NDV) is described experimentally using chicken red blood cells (RBCs). The method proved to be reliable and effective, since infectious stocks of AF2240 strains of NDV were detected after the experimental procedure. To detect...
| المؤلفون الرئيسيون: | , , , |
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| التنسيق: | مقال |
| اللغة: | English |
| منشور في: |
Scholar Science Journals
2014
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| الوصول للمادة أونلاين: | http://psasir.upm.edu.my/id/eprint/37329/1/Effectiveness%20of%20Haemagglutination%20test%20in%20detection%20of%20Newcastle%20disease%20virus.pdf |
| _version_ | 1825949014480125952 |
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| author | Ahmad, Umar Ahmed, Ismaila Othman, Fauziah Young, Yoke Keong |
| author_facet | Ahmad, Umar Ahmed, Ismaila Othman, Fauziah Young, Yoke Keong |
| author_sort | Ahmad, Umar |
| collection | UPM |
| description | Haemagglutination (HA) test for the detection of Newcastle disease virus (NDV) is described experimentally using chicken red blood cells (RBCs). The method proved to be reliable and effective, since infectious stocks of AF2240 strains of NDV were detected after the experimental procedure. To detect NDV in chicken allantoic fluids of SPF eggs, 0.1 mL of NDV AF2240 (velogenic) virus stock were inoculated into each egg. The virus was allowed to propagate for production of more stock and the unclarified allantoic fluids were harvested aseptically and tested for confirmation of the virus presence before the stock virus was further clarified and purified using high speed refrigerated centrifuge following sucrose gradients method. This sucrose gradients were later balanced with NTE buffer and sieved using 0.45 µm filter and the purified samples of the virus were collected followed by HA test to detect the presence and to determine the virus titer. The results showed (28 = 256 HAU/mL) virus titer after purification of the allantoic fluids. |
| first_indexed | 2024-03-06T08:38:03Z |
| format | Article |
| id | upm.eprints-37329 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:38:03Z |
| publishDate | 2014 |
| publisher | Scholar Science Journals |
| record_format | dspace |
| spelling | upm.eprints-373292015-08-27T08:09:22Z http://psasir.upm.edu.my/id/eprint/37329/ Effectiveness of Haemagglutination test in detection of Newcastle disease virus (NDV) AF2240 strain from harvested allantoic fluids of infected chicken eggs Ahmad, Umar Ahmed, Ismaila Othman, Fauziah Young, Yoke Keong Haemagglutination (HA) test for the detection of Newcastle disease virus (NDV) is described experimentally using chicken red blood cells (RBCs). The method proved to be reliable and effective, since infectious stocks of AF2240 strains of NDV were detected after the experimental procedure. To detect NDV in chicken allantoic fluids of SPF eggs, 0.1 mL of NDV AF2240 (velogenic) virus stock were inoculated into each egg. The virus was allowed to propagate for production of more stock and the unclarified allantoic fluids were harvested aseptically and tested for confirmation of the virus presence before the stock virus was further clarified and purified using high speed refrigerated centrifuge following sucrose gradients method. This sucrose gradients were later balanced with NTE buffer and sieved using 0.45 µm filter and the purified samples of the virus were collected followed by HA test to detect the presence and to determine the virus titer. The results showed (28 = 256 HAU/mL) virus titer after purification of the allantoic fluids. Scholar Science Journals 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37329/1/Effectiveness%20of%20Haemagglutination%20test%20in%20detection%20of%20Newcastle%20disease%20virus.pdf Ahmad, Umar and Ahmed, Ismaila and Othman, Fauziah and Young, Yoke Keong (2014) Effectiveness of Haemagglutination test in detection of Newcastle disease virus (NDV) AF2240 strain from harvested allantoic fluids of infected chicken eggs. International Journal of Biomedical Research, 5 (10). pp. 606-609. ISSN 0976-9633 http://www.ssjournals.com/index.php/ijbr/article/view/1083 10.7439/ijbr.v5i10.751 |
| spellingShingle | Ahmad, Umar Ahmed, Ismaila Othman, Fauziah Young, Yoke Keong Effectiveness of Haemagglutination test in detection of Newcastle disease virus (NDV) AF2240 strain from harvested allantoic fluids of infected chicken eggs |
| title | Effectiveness of Haemagglutination test in detection of Newcastle disease virus (NDV) AF2240 strain from harvested allantoic fluids of infected chicken eggs |
| title_full | Effectiveness of Haemagglutination test in detection of Newcastle disease virus (NDV) AF2240 strain from harvested allantoic fluids of infected chicken eggs |
| title_fullStr | Effectiveness of Haemagglutination test in detection of Newcastle disease virus (NDV) AF2240 strain from harvested allantoic fluids of infected chicken eggs |
| title_full_unstemmed | Effectiveness of Haemagglutination test in detection of Newcastle disease virus (NDV) AF2240 strain from harvested allantoic fluids of infected chicken eggs |
| title_short | Effectiveness of Haemagglutination test in detection of Newcastle disease virus (NDV) AF2240 strain from harvested allantoic fluids of infected chicken eggs |
| title_sort | effectiveness of haemagglutination test in detection of newcastle disease virus ndv af2240 strain from harvested allantoic fluids of infected chicken eggs |
| url | http://psasir.upm.edu.my/id/eprint/37329/1/Effectiveness%20of%20Haemagglutination%20test%20in%20detection%20of%20Newcastle%20disease%20virus.pdf |
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