Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia
Streptococcus pneumoniae is an epidemiologically important bacterial pathogen. Recently, we reported the antibiotic susceptibility patterns of a limited collection of pneumococcal isolates in Malaysia with a high prevalence of erythromycin resistant strains. In the present study, 55 of the pneumococ...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37767/1/37767.pdf |
| _version_ | 1796973212675866624 |
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| author | Jeevajothi Nathan, Jayakayatri Mohd Desa, Mohd Nasir Thong, Kwai Lin Clarke, Stuart C. Masri, Siti Norbaya Md Yasin, Rohani Mohd Taib, Niazlin |
| author_facet | Jeevajothi Nathan, Jayakayatri Mohd Desa, Mohd Nasir Thong, Kwai Lin Clarke, Stuart C. Masri, Siti Norbaya Md Yasin, Rohani Mohd Taib, Niazlin |
| author_sort | Jeevajothi Nathan, Jayakayatri |
| collection | UPM |
| description | Streptococcus pneumoniae is an epidemiologically important bacterial pathogen. Recently, we reported the antibiotic susceptibility patterns of a limited collection of pneumococcal isolates in Malaysia with a high prevalence of erythromycin resistant strains. In the present study, 55 of the pneumococcal isolates of serotype 19F were further analysed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The generated genotypic patterns were then correlated with the antibiograms previously reported. Forty-seven different PFGE profiles (PTs) were obtained, showing that the isolates were genetically diverse. MLST identified 16 sequence types (STs) with ST-236 being predominant (58.2%), followed by ST-81 (10.3%). Among the ST-236 isolates, 22 were erythromycin resistant S. pneumoniae (ERSP) and 15 were trimethoprim/sulfamethoxazole (TMP/SMX) resistant, while among ST-81, four isolates were ERSP and two were TMP/SMX resistant. The high prevalence of erythromycin resistant serotype 19F isolates of ST-236 in this study has also been reported in other North and South East Asian countries. |
| first_indexed | 2024-03-06T08:39:19Z |
| format | Article |
| id | upm.eprints-37767 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:39:19Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-377672016-04-22T02:36:33Z http://psasir.upm.edu.my/id/eprint/37767/ Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia Jeevajothi Nathan, Jayakayatri Mohd Desa, Mohd Nasir Thong, Kwai Lin Clarke, Stuart C. Masri, Siti Norbaya Md Yasin, Rohani Mohd Taib, Niazlin Streptococcus pneumoniae is an epidemiologically important bacterial pathogen. Recently, we reported the antibiotic susceptibility patterns of a limited collection of pneumococcal isolates in Malaysia with a high prevalence of erythromycin resistant strains. In the present study, 55 of the pneumococcal isolates of serotype 19F were further analysed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The generated genotypic patterns were then correlated with the antibiograms previously reported. Forty-seven different PFGE profiles (PTs) were obtained, showing that the isolates were genetically diverse. MLST identified 16 sequence types (STs) with ST-236 being predominant (58.2%), followed by ST-81 (10.3%). Among the ST-236 isolates, 22 were erythromycin resistant S. pneumoniae (ERSP) and 15 were trimethoprim/sulfamethoxazole (TMP/SMX) resistant, while among ST-81, four isolates were ERSP and two were TMP/SMX resistant. The high prevalence of erythromycin resistant serotype 19F isolates of ST-236 in this study has also been reported in other North and South East Asian countries. Elsevier 2014-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37767/1/37767.pdf Jeevajothi Nathan, Jayakayatri and Mohd Desa, Mohd Nasir and Thong, Kwai Lin and Clarke, Stuart C. and Masri, Siti Norbaya and Md Yasin, Rohani and Mohd Taib, Niazlin (2014) Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia. Infection, Genetics and Evolution, 21. pp. 391-394. ISSN 1567-1348; ESSN: 1567-7257 http://www.sciencedirect.com/science/article/pii/S1567134813004437 10.1016/j.meegid.2013.11.026 |
| spellingShingle | Jeevajothi Nathan, Jayakayatri Mohd Desa, Mohd Nasir Thong, Kwai Lin Clarke, Stuart C. Masri, Siti Norbaya Md Yasin, Rohani Mohd Taib, Niazlin Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia |
| title | Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia |
| title_full | Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia |
| title_fullStr | Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia |
| title_full_unstemmed | Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia |
| title_short | Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia |
| title_sort | genotypic characterization of streptococcus pneumoniae serotype 19f in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/37767/1/37767.pdf |
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