High-risk human papillomavirus infection in different histological subtypes of renal cell carcinoma
Limited data exist regarding whether a high-risk human papillomavirus (HR-HPV) infection increases the risk of developing renal cell carcinoma. The aim of this study was to investigate whether HPV infection has a role in the pathogenesis or development of a certain histological subtype of renal cell...
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| Format: | Article |
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Wiley Periodicals
2014
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| _version_ | 1825949115221016576 |
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| author | Farhadi, Ali Behbahani, Abbas Behzad Geramizadeh, Bita Sekawi, Zamberi Rahsaz, Marjan Sharifzadeh, Sedigheh |
| author_facet | Farhadi, Ali Behbahani, Abbas Behzad Geramizadeh, Bita Sekawi, Zamberi Rahsaz, Marjan Sharifzadeh, Sedigheh |
| author_sort | Farhadi, Ali |
| collection | UPM |
| description | Limited data exist regarding whether a high-risk human papillomavirus (HR-HPV) infection increases the risk of developing renal cell carcinoma. The aim of this study was to investigate whether HPV infection has a role in the pathogenesis or development of a certain histological subtype of renal cell carcinoma. Formalin-fixed paraffin-embedded (FFPE) specimens of 122 patients with histopathologically proven renal cell carcinoma and their respective peritumoral tissues were examined. The presence of HPV-DNA was determined by a combination of MY/GP+ consensus primers and HPV-16/18 type specific nested PCRs followed by direct sequencing. Catalyzed signal-amplified colorimetric in situ hybridization (CSAC-ISH) technique was applied to determine the physical status of viral genome. The expression of p16INK4a and HPV L1 capsid proteins was evaluated using immunohistochemistry. HPV genome was detected in 37 (30.3%) tumor specimens and their four (4.1%) corresponding peritumoral tissues. HPV-18 was the most common viral type identified followed by HPV-16 and 58. Immunoexpression of p16INK4a was detected in 24 (20.3%) cases. Data analysis showed a significant correlation between p16INK4a expression and the presence of HR-HPV DNA (P < 0.001). CSAC-ISH analysis confirmed HR-HPV infection in 45% of tumors, which were previously tested positive for HPV-DNA. Diffuse signal pattern was identified in 15 (83.3%) samples whereas a mixed pattern of diffuse and punctate signals was only detectable in three cases. The results indicate an association of HR-HPV types with renal cell carcinoma. It is proposed that HPV infection in high-grade tumors might precede disease progression in a number of tumors, particularly of the papillary subtype. |
| first_indexed | 2024-03-06T08:39:36Z |
| format | Article |
| id | upm.eprints-37866 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:39:36Z |
| publishDate | 2014 |
| publisher | Wiley Periodicals |
| record_format | dspace |
| spelling | upm.eprints-378662015-09-25T01:28:57Z http://psasir.upm.edu.my/id/eprint/37866/ High-risk human papillomavirus infection in different histological subtypes of renal cell carcinoma Farhadi, Ali Behbahani, Abbas Behzad Geramizadeh, Bita Sekawi, Zamberi Rahsaz, Marjan Sharifzadeh, Sedigheh Limited data exist regarding whether a high-risk human papillomavirus (HR-HPV) infection increases the risk of developing renal cell carcinoma. The aim of this study was to investigate whether HPV infection has a role in the pathogenesis or development of a certain histological subtype of renal cell carcinoma. Formalin-fixed paraffin-embedded (FFPE) specimens of 122 patients with histopathologically proven renal cell carcinoma and their respective peritumoral tissues were examined. The presence of HPV-DNA was determined by a combination of MY/GP+ consensus primers and HPV-16/18 type specific nested PCRs followed by direct sequencing. Catalyzed signal-amplified colorimetric in situ hybridization (CSAC-ISH) technique was applied to determine the physical status of viral genome. The expression of p16INK4a and HPV L1 capsid proteins was evaluated using immunohistochemistry. HPV genome was detected in 37 (30.3%) tumor specimens and their four (4.1%) corresponding peritumoral tissues. HPV-18 was the most common viral type identified followed by HPV-16 and 58. Immunoexpression of p16INK4a was detected in 24 (20.3%) cases. Data analysis showed a significant correlation between p16INK4a expression and the presence of HR-HPV DNA (P < 0.001). CSAC-ISH analysis confirmed HR-HPV infection in 45% of tumors, which were previously tested positive for HPV-DNA. Diffuse signal pattern was identified in 15 (83.3%) samples whereas a mixed pattern of diffuse and punctate signals was only detectable in three cases. The results indicate an association of HR-HPV types with renal cell carcinoma. It is proposed that HPV infection in high-grade tumors might precede disease progression in a number of tumors, particularly of the papillary subtype. Wiley Periodicals 2014-07 Article PeerReviewed Farhadi, Ali and Behbahani, Abbas Behzad and Geramizadeh, Bita and Sekawi, Zamberi and Rahsaz, Marjan and Sharifzadeh, Sedigheh (2014) High-risk human papillomavirus infection in different histological subtypes of renal cell carcinoma. Journal of Medical Virology, 86 (7). pp. 1134-1144. ISSN 0146-6615; ESSN: 1096-9071 http://onlinelibrary.wiley.com/doi/10.1002/jmv.23945/abstract;jsessionid=F558D9E27012B3915E023131D1F90EBF.f02t03 10.1002/jmv.23945 |
| spellingShingle | Farhadi, Ali Behbahani, Abbas Behzad Geramizadeh, Bita Sekawi, Zamberi Rahsaz, Marjan Sharifzadeh, Sedigheh High-risk human papillomavirus infection in different histological subtypes of renal cell carcinoma |
| title | High-risk human papillomavirus infection in different histological subtypes of renal cell carcinoma |
| title_full | High-risk human papillomavirus infection in different histological subtypes of renal cell carcinoma |
| title_fullStr | High-risk human papillomavirus infection in different histological subtypes of renal cell carcinoma |
| title_full_unstemmed | High-risk human papillomavirus infection in different histological subtypes of renal cell carcinoma |
| title_short | High-risk human papillomavirus infection in different histological subtypes of renal cell carcinoma |
| title_sort | high risk human papillomavirus infection in different histological subtypes of renal cell carcinoma |
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