Identification of Pithomyces chartarum causing leaf spot of cabbage in Malaysia

Identification of Pithomyces chartarum causing leaf spot of cabbage in Malaysia

A leaf spot disease was observed on cabbage (Brassica oleracea L. var. capitata) affecting 80% of plants growing in greenhouses and fields in Serdang, Selangor, Malaysia. Symptomatic leaf samples were collected from infected plants and isolations made on agar medium. Single-spore isolates from resul...

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Bibliographic Details
Main Authors: Nasehi, Abbas, Kadir, Jugah, Ashtiani, Farnaz Abed, Esfahani, Mehdi Nasr, Golkhandan, Elham, Nyaka Ngobisa, A. I. C.
Format: Article
Language:English
Published: Taylor & Francis 2014
Online Access:http://psasir.upm.edu.my/id/eprint/37922/1/Identification%20of%20Pithomyces%20chartarum%20causing%20leaf%20spot%20of%20cabbage%20in%20Malaysia.pdf
Description
Summary:A leaf spot disease was observed on cabbage (Brassica oleracea L. var. capitata) affecting 80% of plants growing in greenhouses and fields in Serdang, Selangor, Malaysia. Symptomatic leaf samples were collected from infected plants and isolations made on agar medium. Single-spore isolates from resulting colonies were identified based on cultural and morphological characteristics as Pithomyces chartarum. Morphological identification was confirmed by sequence analysis of the internal transcribed spacer (ITS) regions 1 and 2, including 5.8S rDNA (ITS1-5.8S-ITS2). Pathogenicity tests indicated that P. chartarum causes leaf spot on cabbage. This is the first report of leaf spot caused by P. chartarum on cabbage in Malaysia.

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