Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin
Background: Tamm-Horsfall glycoprotein (THP) and uromodulin are the most abundant glycoproteins in non-pregnant women’s/men’s and pregnant women’s urine, respectively. However, the bioactivities of these glycoproteins are still unclear. Objective: To evaluate the immunomodulatory properties of THP a...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Allergy, Asthma and Immunology Association of Thailand
2015
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/37938/1/Immunomodulatory%20properties%20of%20Tamm-Horsfall%20glycoprotein%20%28THP%29%20and%20uromodulin.pdf |
| _version_ | 1796973246342496256 |
|---|---|
| author | Hong, Chia Yean Wong, Nyet Kui Abdullah, Maha |
| author_facet | Hong, Chia Yean Wong, Nyet Kui Abdullah, Maha |
| author_sort | Hong, Chia Yean |
| collection | UPM |
| description | Background: Tamm-Horsfall glycoprotein (THP) and uromodulin are the most abundant glycoproteins in non-pregnant women’s/men’s and pregnant women’s urine, respectively. However, the bioactivities of these glycoproteins are still unclear. Objective: To evaluate the immunomodulatory properties of THP and uromodulin on human peripheral blood mononuclear cells (PBMC). Methods: THP and uromodulin isolated with diatomaceous earth filtration were subjected to several bioassays, such as MTS viability assay, immunophenotyping and cytokine analysis. Results: MTS viability assay and immunophenotyping analysis showed that uromodulin has greater inhibitory activities in suppressing PBMC viability and the percentage of CD4+ T helper cells and CD8+ cytotoxic T cells, compared to that of THP. In cytokine analysis, THP tended to induce pro-inflammatory cytokines such as IL-1β, TNF and Th1 cytokine IFN-γ; while uromodulin only induced IL-1β and suppressed both Th1 cytokine IFN-γ and Th2 cytokine IL-10. Conclusion: These results demonstrate that uromodulin has greater immunosuppressive activities and lower inductive property in relation to activation of immune cells, which provides a more tolerant environment for the developing fetus. |
| first_indexed | 2024-03-06T08:39:49Z |
| format | Article |
| id | upm.eprints-37938 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:39:49Z |
| publishDate | 2015 |
| publisher | Allergy, Asthma and Immunology Association of Thailand |
| record_format | dspace |
| spelling | upm.eprints-379382015-12-29T12:26:44Z http://psasir.upm.edu.my/id/eprint/37938/ Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin Hong, Chia Yean Wong, Nyet Kui Abdullah, Maha Background: Tamm-Horsfall glycoprotein (THP) and uromodulin are the most abundant glycoproteins in non-pregnant women’s/men’s and pregnant women’s urine, respectively. However, the bioactivities of these glycoproteins are still unclear. Objective: To evaluate the immunomodulatory properties of THP and uromodulin on human peripheral blood mononuclear cells (PBMC). Methods: THP and uromodulin isolated with diatomaceous earth filtration were subjected to several bioassays, such as MTS viability assay, immunophenotyping and cytokine analysis. Results: MTS viability assay and immunophenotyping analysis showed that uromodulin has greater inhibitory activities in suppressing PBMC viability and the percentage of CD4+ T helper cells and CD8+ cytotoxic T cells, compared to that of THP. In cytokine analysis, THP tended to induce pro-inflammatory cytokines such as IL-1β, TNF and Th1 cytokine IFN-γ; while uromodulin only induced IL-1β and suppressed both Th1 cytokine IFN-γ and Th2 cytokine IL-10. Conclusion: These results demonstrate that uromodulin has greater immunosuppressive activities and lower inductive property in relation to activation of immune cells, which provides a more tolerant environment for the developing fetus. Allergy, Asthma and Immunology Association of Thailand 2015-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37938/1/Immunomodulatory%20properties%20of%20Tamm-Horsfall%20glycoprotein%20%28THP%29%20and%20uromodulin.pdf Hong, Chia Yean and Wong, Nyet Kui and Abdullah, Maha (2015) Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin. Asian Pacific Journal of Allergy and Immunology, 33 (1). pp. 26-32. ISSN 0125-877X http://thailand.digitaljournals.org/index.php/APJAI/article/view/27305 |
| spellingShingle | Hong, Chia Yean Wong, Nyet Kui Abdullah, Maha Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin |
| title | Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin |
| title_full | Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin |
| title_fullStr | Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin |
| title_full_unstemmed | Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin |
| title_short | Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin |
| title_sort | immunomodulatory properties of tamm horsfall glycoprotein thp and uromodulin |
| url | http://psasir.upm.edu.my/id/eprint/37938/1/Immunomodulatory%20properties%20of%20Tamm-Horsfall%20glycoprotein%20%28THP%29%20and%20uromodulin.pdf |
| work_keys_str_mv | AT hongchiayean immunomodulatorypropertiesoftammhorsfallglycoproteinthpanduromodulin AT wongnyetkui immunomodulatorypropertiesoftammhorsfallglycoproteinthpanduromodulin AT abdullahmaha immunomodulatorypropertiesoftammhorsfallglycoproteinthpanduromodulin |