In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao
The aims of this study were to determine the antioxidant and antiproliferative activity of the following Theobroma cacao plant part methanolic extracts: leaf, bark, husk, fermented and unfermented shell, pith, root, and cherelle. Antioxidant activity was determined using 2,2-diphenyl-2-picrylhydrazy...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38002/1/38002.pdf |
| _version_ | 1825949140820951040 |
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| author | Baharum, Zainal Md. Akim, Abdah Yap, Taufiq Yun Hin Abd Hamid @ Abdul Razak, Roslida Kasran, Rosmin |
| author_facet | Baharum, Zainal Md. Akim, Abdah Yap, Taufiq Yun Hin Abd Hamid @ Abdul Razak, Roslida Kasran, Rosmin |
| author_sort | Baharum, Zainal |
| collection | UPM |
| description | The aims of this study were to determine the antioxidant and antiproliferative activity of the following Theobroma cacao plant part methanolic extracts: leaf, bark, husk, fermented and unfermented shell, pith, root, and cherelle. Antioxidant activity was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH), thiobarbituric acid-reactive substances (TBARS), and Folin-Ciocalteu assays; the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay was used to determine antiproliferative activity. The root extract had the highest antioxidant activity; its median effective dose (EC50) was 358.3 ± 7.0 µg/mL and total phenolic content was 22.0 ± 1.1 g GAE/100 g extract as compared to the other methanolic plant part extracts. Only the cherelle extract demonstrated 10.4% ± 1.1% inhibition activity in the lipid peroxidation assay. The MTT assay revealed that the leaf extract had the highest antiproliferative activity against MCF-7 cells [median inhibitory concentration (IC50) = 41.4 ± 3.3 µg/mL]. Given the overall high IC50 for the normal liver cell line WRL-68, this study indicates that T. cacao methanolic extracts have a cytotoxic effect in cancer cells, but not in normal cells. Planned future investigations will involve the purification, identification, determination of the mechanisms of action, and molecular assay of T. cacao plant extracts. |
| first_indexed | 2024-03-06T08:40:00Z |
| format | Article |
| id | upm.eprints-38002 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:00Z |
| publishDate | 2014 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-380022016-10-31T02:02:25Z http://psasir.upm.edu.my/id/eprint/38002/ In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao Baharum, Zainal Md. Akim, Abdah Yap, Taufiq Yun Hin Abd Hamid @ Abdul Razak, Roslida Kasran, Rosmin The aims of this study were to determine the antioxidant and antiproliferative activity of the following Theobroma cacao plant part methanolic extracts: leaf, bark, husk, fermented and unfermented shell, pith, root, and cherelle. Antioxidant activity was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH), thiobarbituric acid-reactive substances (TBARS), and Folin-Ciocalteu assays; the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay was used to determine antiproliferative activity. The root extract had the highest antioxidant activity; its median effective dose (EC50) was 358.3 ± 7.0 µg/mL and total phenolic content was 22.0 ± 1.1 g GAE/100 g extract as compared to the other methanolic plant part extracts. Only the cherelle extract demonstrated 10.4% ± 1.1% inhibition activity in the lipid peroxidation assay. The MTT assay revealed that the leaf extract had the highest antiproliferative activity against MCF-7 cells [median inhibitory concentration (IC50) = 41.4 ± 3.3 µg/mL]. Given the overall high IC50 for the normal liver cell line WRL-68, this study indicates that T. cacao methanolic extracts have a cytotoxic effect in cancer cells, but not in normal cells. Planned future investigations will involve the purification, identification, determination of the mechanisms of action, and molecular assay of T. cacao plant extracts. MDPI 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/38002/1/38002.pdf Baharum, Zainal and Md. Akim, Abdah and Yap, Taufiq Yun Hin and Abd Hamid @ Abdul Razak, Roslida and Kasran, Rosmin (2014) In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao. Molecules, 19 (11). pp. 18317-18331. ISSN 1420-3049 http://www.mdpi.com/1420-3049/19/11/18317 10.3390/molecules191118317 |
| spellingShingle | Baharum, Zainal Md. Akim, Abdah Yap, Taufiq Yun Hin Abd Hamid @ Abdul Razak, Roslida Kasran, Rosmin In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao |
| title | In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao |
| title_full | In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao |
| title_fullStr | In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao |
| title_full_unstemmed | In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao |
| title_short | In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao |
| title_sort | in vitro antioxidant and antiproliferative activities of methanolic plant part extracts of theobroma cacao |
| url | http://psasir.upm.edu.my/id/eprint/38002/1/38002.pdf |
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