In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards feline infectious peritonitis virus replication
Feline Infectious Peritonitis (FIP) is a severe fatal immune-augmented disease in cat population. It is caused by FIP virus (FIPV), a virulent mutant strain of Feline Enteric Coronavirus (FECV). Current treatments and prophylactics are not effective. The in vitro antiviral properties of five circula...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38005/1/38005.pdf |
| _version_ | 1825949141542371328 |
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| author | Choong, Oi Kuan Mehrbod, Parvaneh Tejo, Bimo Ario Omar, Abdul Rahman |
| author_facet | Choong, Oi Kuan Mehrbod, Parvaneh Tejo, Bimo Ario Omar, Abdul Rahman |
| author_sort | Choong, Oi Kuan |
| collection | UPM |
| description | Feline Infectious Peritonitis (FIP) is a severe fatal immune-augmented disease in cat population. It is caused by FIP virus (FIPV), a virulent mutant strain of Feline Enteric Coronavirus (FECV). Current treatments and prophylactics are not effective. The in vitro antiviral properties of five circular Triple-Helix Forming Oligonucleotide (TFO) RNAs (TFO1 to TFO5), which target the different regions of virulent feline coronavirus (FCoV) strain FIPV WSU 79-1146 genome, were tested in FIPV-infected Crandell-Rees Feline Kidney (CRFK) cells. RT-qPCR results showed that the circular TFO RNAs, except TFO2, inhibit FIPV replication, where the viral genome copy numbers decreased significantly by 5-fold log10 from 1014 in the virus-inoculated cells to 109 in the circular TFO RNAs-transfected cells. Furthermore, the binding of the circular TFO RNA with the targeted viral genome segment was also confirmed using electrophoretic mobility shift assay. The strength of binding kinetics between the TFO RNAs and their target regions was demonstrated by NanoITC assay. In conclusion, the circular TFOs have the potential to be further developed as antiviral agents against FIPV infection. |
| first_indexed | 2024-03-06T08:40:00Z |
| format | Article |
| id | upm.eprints-38005 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:00Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-380052015-12-18T07:51:13Z http://psasir.upm.edu.my/id/eprint/38005/ In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards feline infectious peritonitis virus replication Choong, Oi Kuan Mehrbod, Parvaneh Tejo, Bimo Ario Omar, Abdul Rahman Feline Infectious Peritonitis (FIP) is a severe fatal immune-augmented disease in cat population. It is caused by FIP virus (FIPV), a virulent mutant strain of Feline Enteric Coronavirus (FECV). Current treatments and prophylactics are not effective. The in vitro antiviral properties of five circular Triple-Helix Forming Oligonucleotide (TFO) RNAs (TFO1 to TFO5), which target the different regions of virulent feline coronavirus (FCoV) strain FIPV WSU 79-1146 genome, were tested in FIPV-infected Crandell-Rees Feline Kidney (CRFK) cells. RT-qPCR results showed that the circular TFO RNAs, except TFO2, inhibit FIPV replication, where the viral genome copy numbers decreased significantly by 5-fold log10 from 1014 in the virus-inoculated cells to 109 in the circular TFO RNAs-transfected cells. Furthermore, the binding of the circular TFO RNA with the targeted viral genome segment was also confirmed using electrophoretic mobility shift assay. The strength of binding kinetics between the TFO RNAs and their target regions was demonstrated by NanoITC assay. In conclusion, the circular TFOs have the potential to be further developed as antiviral agents against FIPV infection. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/38005/1/38005.pdf Choong, Oi Kuan and Mehrbod, Parvaneh and Tejo, Bimo Ario and Omar, Abdul Rahman (2014) In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards feline infectious peritonitis virus replication. BioMed Research International, 2014. art. no. 654712. pp. 1-8. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2014/654712/abs/ 10.1155/2014/654712 |
| spellingShingle | Choong, Oi Kuan Mehrbod, Parvaneh Tejo, Bimo Ario Omar, Abdul Rahman In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards feline infectious peritonitis virus replication |
| title | In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards feline infectious peritonitis virus replication |
| title_full | In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards feline infectious peritonitis virus replication |
| title_fullStr | In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards feline infectious peritonitis virus replication |
| title_full_unstemmed | In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards feline infectious peritonitis virus replication |
| title_short | In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards feline infectious peritonitis virus replication |
| title_sort | in vitro antiviral activity of circular triple helix forming oligonucleotide rna towards feline infectious peritonitis virus replication |
| url | http://psasir.upm.edu.my/id/eprint/38005/1/38005.pdf |
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