Sandwich-type DNA micro-optode based on gold–latex spheres label for reflectance dengue virus detection
A DNA micro-optode for dengue virus detection was developed based on the sandwich hybridization strategy of DNAs on succinimide-functionalized poly(n-butyl acrylate) (poly(nBA-NAS)) microspheres. Gold nanoparticles (AuNPs) with an average diameter of ~20 nm were synthesized using a centrifugation-ba...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38106/1/38106.pdf |
| _version_ | 1825949161340534784 |
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| author | Jeningsih Tan, Ling Ling Ulianas, Alizar Lee, Yook Heng Mazlan, Nur-Fadhilah Jamaluddin, Nur Diyana Mohd Yusof, Nurul Yuziana Khalid, Bahariah Goh, Choo Ta |
| author_facet | Jeningsih Tan, Ling Ling Ulianas, Alizar Lee, Yook Heng Mazlan, Nur-Fadhilah Jamaluddin, Nur Diyana Mohd Yusof, Nurul Yuziana Khalid, Bahariah Goh, Choo Ta |
| author_sort | Jeningsih |
| collection | UPM |
| description | A DNA micro-optode for dengue virus detection was developed based on the sandwich hybridization strategy of DNAs on succinimide-functionalized poly(n-butyl acrylate) (poly(nBA-NAS)) microspheres. Gold nanoparticles (AuNPs) with an average diameter of ~20 nm were synthesized using a centrifugation-based method and adsorbed on the submicrometer-sized polyelectrolyte-coated poly(styrene-co-acrylic acid) (PSA) latex particles via an electrostatic method. The AuNP–latex spheres were attached to the thiolated reporter probe (rDNA) by Au–thiol binding to functionalize as an optical gold–latex–rDNA label. The one-step sandwich hybridization recognition involved a pair of a DNA probe, i.e., capture probe (pDNA), and AuNP–PSA reporter label that flanked the target DNA (complementary DNA (cDNA)). The concentration of dengue virus cDNA was optically transduced by immobilized AuNP–PSA–rDNA conjugates as the DNA micro-optode exhibited a violet hue upon the DNA sandwich hybridization reaction, which could be monitored by a fiber-optic reflectance spectrophotometer at 637 nm. The optical genosensor showed a linear reflectance response over a wide cDNA concentration range from 1.0 × 10−21 M to 1.0 × 10−12 M cDNA (R2 = 0.9807) with a limit of detection (LOD) of 1 × 10−29 M. The DNA biosensor was reusable for three consecutive applications after regeneration with mild sodium hydroxide. The sandwich-type optical biosensor was well validated with a molecular reverse transcription polymerase chain reaction (RT-PCR) technique for screening of dengue virus in clinical samples, e.g., serum, urine, and saliva from dengue virus-infected patients under informed consent. |
| first_indexed | 2024-03-06T08:40:19Z |
| format | Article |
| id | upm.eprints-38106 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:19Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-381062020-04-14T13:58:37Z http://psasir.upm.edu.my/id/eprint/38106/ Sandwich-type DNA micro-optode based on gold–latex spheres label for reflectance dengue virus detection Jeningsih Tan, Ling Ling Ulianas, Alizar Lee, Yook Heng Mazlan, Nur-Fadhilah Jamaluddin, Nur Diyana Mohd Yusof, Nurul Yuziana Khalid, Bahariah Goh, Choo Ta A DNA micro-optode for dengue virus detection was developed based on the sandwich hybridization strategy of DNAs on succinimide-functionalized poly(n-butyl acrylate) (poly(nBA-NAS)) microspheres. Gold nanoparticles (AuNPs) with an average diameter of ~20 nm were synthesized using a centrifugation-based method and adsorbed on the submicrometer-sized polyelectrolyte-coated poly(styrene-co-acrylic acid) (PSA) latex particles via an electrostatic method. The AuNP–latex spheres were attached to the thiolated reporter probe (rDNA) by Au–thiol binding to functionalize as an optical gold–latex–rDNA label. The one-step sandwich hybridization recognition involved a pair of a DNA probe, i.e., capture probe (pDNA), and AuNP–PSA reporter label that flanked the target DNA (complementary DNA (cDNA)). The concentration of dengue virus cDNA was optically transduced by immobilized AuNP–PSA–rDNA conjugates as the DNA micro-optode exhibited a violet hue upon the DNA sandwich hybridization reaction, which could be monitored by a fiber-optic reflectance spectrophotometer at 637 nm. The optical genosensor showed a linear reflectance response over a wide cDNA concentration range from 1.0 × 10−21 M to 1.0 × 10−12 M cDNA (R2 = 0.9807) with a limit of detection (LOD) of 1 × 10−29 M. The DNA biosensor was reusable for three consecutive applications after regeneration with mild sodium hydroxide. The sandwich-type optical biosensor was well validated with a molecular reverse transcription polymerase chain reaction (RT-PCR) technique for screening of dengue virus in clinical samples, e.g., serum, urine, and saliva from dengue virus-infected patients under informed consent. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38106/1/38106.pdf Jeningsih and Tan, Ling Ling and Ulianas, Alizar and Lee, Yook Heng and Mazlan, Nur-Fadhilah and Jamaluddin, Nur Diyana and Mohd Yusof, Nurul Yuziana and Khalid, Bahariah and Goh, Choo Ta (2020) Sandwich-type DNA micro-optode based on gold–latex spheres label for reflectance dengue virus detection. Sensors, 20 (7). art. no. 1820. pp. 1-17. ISSN 1424-8220 https://www.mdpi.com/1424-8220/20/7/1820 10.3390/s20071820 |
| spellingShingle | Jeningsih Tan, Ling Ling Ulianas, Alizar Lee, Yook Heng Mazlan, Nur-Fadhilah Jamaluddin, Nur Diyana Mohd Yusof, Nurul Yuziana Khalid, Bahariah Goh, Choo Ta Sandwich-type DNA micro-optode based on gold–latex spheres label for reflectance dengue virus detection |
| title | Sandwich-type DNA micro-optode based on gold–latex spheres label for reflectance dengue virus detection |
| title_full | Sandwich-type DNA micro-optode based on gold–latex spheres label for reflectance dengue virus detection |
| title_fullStr | Sandwich-type DNA micro-optode based on gold–latex spheres label for reflectance dengue virus detection |
| title_full_unstemmed | Sandwich-type DNA micro-optode based on gold–latex spheres label for reflectance dengue virus detection |
| title_short | Sandwich-type DNA micro-optode based on gold–latex spheres label for reflectance dengue virus detection |
| title_sort | sandwich type dna micro optode based on gold latex spheres label for reflectance dengue virus detection |
| url | http://psasir.upm.edu.my/id/eprint/38106/1/38106.pdf |
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