Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples
An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method, in combination with a mixed matrix membrane microextraction method for the quantification of nonsteroidal anti-inflammatory drugs (NSAIDs) in environmental water samples, is reported. The extraction device was p...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2020
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/38122/1/38122.pdf |
| _version_ | 1825949164455854080 |
|---|---|
| author | Ganesan, Thipashini Mukhtar, Nurul Hazirah Lim, Hong Ngee See, Hong Heng |
| author_facet | Ganesan, Thipashini Mukhtar, Nurul Hazirah Lim, Hong Ngee See, Hong Heng |
| author_sort | Ganesan, Thipashini |
| collection | UPM |
| description | An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method, in combination with a mixed matrix membrane microextraction method for the quantification of nonsteroidal anti-inflammatory drugs (NSAIDs) in environmental water samples, is reported. The extraction device was prepared by casting well-dispersed polymeric bonded octadecyl (C18) particles in a cellulose triacetate matrix solution onto commercially available 200 μL micropipette tips. The membrane formed contains 25% of the adsorbent loading amount and was firmly attached to the inner wall of the membrane tip. The dynamic extraction was performed by withdrawing and dispensing the sample solution through the tip device for effective analyte adsorption, followed by the analyte desorption process into 40 μL of methanol and acetonitrile (1:1) prior to UPLC–MS/MS analysis. NSAIDs—namely diclofenac, ibuprofen, indoprofen, naproxen and sulindac—were chosen as targeted analytes. Several extraction parameters were comprehensively optimized, including sample pH value, ionic strength, dynamic extraction cycle, desorption solvent and desorption time. The optimized conditions demonstrated a linear range from 0.25 to 500 ng L−1, with correlation coefficients (r2) from 0.9988 to 0.9992 and detection limits ranging from 0.08 to 0.40 ng L−1. The recoveries of the spiked water samples were between 92% and 99% and exhibited excellent precision relative to standard deviations (RSDs ≤ 4.9%), and enrichment factors (EFs) were at 201–249 for the developed approach. |
| first_indexed | 2024-03-06T08:40:21Z |
| format | Article |
| id | upm.eprints-38122 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:21Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-381222020-05-03T22:52:38Z http://psasir.upm.edu.my/id/eprint/38122/ Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples Ganesan, Thipashini Mukhtar, Nurul Hazirah Lim, Hong Ngee See, Hong Heng An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method, in combination with a mixed matrix membrane microextraction method for the quantification of nonsteroidal anti-inflammatory drugs (NSAIDs) in environmental water samples, is reported. The extraction device was prepared by casting well-dispersed polymeric bonded octadecyl (C18) particles in a cellulose triacetate matrix solution onto commercially available 200 μL micropipette tips. The membrane formed contains 25% of the adsorbent loading amount and was firmly attached to the inner wall of the membrane tip. The dynamic extraction was performed by withdrawing and dispensing the sample solution through the tip device for effective analyte adsorption, followed by the analyte desorption process into 40 μL of methanol and acetonitrile (1:1) prior to UPLC–MS/MS analysis. NSAIDs—namely diclofenac, ibuprofen, indoprofen, naproxen and sulindac—were chosen as targeted analytes. Several extraction parameters were comprehensively optimized, including sample pH value, ionic strength, dynamic extraction cycle, desorption solvent and desorption time. The optimized conditions demonstrated a linear range from 0.25 to 500 ng L−1, with correlation coefficients (r2) from 0.9988 to 0.9992 and detection limits ranging from 0.08 to 0.40 ng L−1. The recoveries of the spiked water samples were between 92% and 99% and exhibited excellent precision relative to standard deviations (RSDs ≤ 4.9%), and enrichment factors (EFs) were at 201–249 for the developed approach. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38122/1/38122.pdf Ganesan, Thipashini and Mukhtar, Nurul Hazirah and Lim, Hong Ngee and See, Hong Heng (2020) Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples. Separations, 7 (1). art. no. 19. pp. 1-10. ISSN 2297-8739 https://www.mdpi.com/2297-8739/7/1/19 10.3390/separations7010019 |
| spellingShingle | Ganesan, Thipashini Mukhtar, Nurul Hazirah Lim, Hong Ngee See, Hong Heng Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples |
| title | Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples |
| title_full | Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples |
| title_fullStr | Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples |
| title_full_unstemmed | Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples |
| title_short | Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples |
| title_sort | mixed matrix membrane tip extraction coupled with uplc ms ms for the monitoring of nonsteroidal anti inflammatory drugs in water samples |
| url | http://psasir.upm.edu.my/id/eprint/38122/1/38122.pdf |
| work_keys_str_mv | AT ganesanthipashini mixedmatrixmembranetipextractioncoupledwithuplcmsmsforthemonitoringofnonsteroidalantiinflammatorydrugsinwatersamples AT mukhtarnurulhazirah mixedmatrixmembranetipextractioncoupledwithuplcmsmsforthemonitoringofnonsteroidalantiinflammatorydrugsinwatersamples AT limhongngee mixedmatrixmembranetipextractioncoupledwithuplcmsmsforthemonitoringofnonsteroidalantiinflammatorydrugsinwatersamples AT seehongheng mixedmatrixmembranetipextractioncoupledwithuplcmsmsforthemonitoringofnonsteroidalantiinflammatorydrugsinwatersamples |