Methanol extract of Dicranopteris linearis leaves attenuate pain via the modulation of opioid/NO-mediated pathway
Dicranopteris linearis leaf has been reported to exert antinociceptive activity. The present study elucidates the possible mechanisms of antinociception modulated by the methanol extract of D. linearis leaves (MEDL) using various mouse models. The extract (25, 150, and 300 mg/kg) was administered or...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38160/1/38160.pdf |
| _version_ | 1796973290539974656 |
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| author | Zakaria, Zainul Amiruddin Roosli, Rushduddin Al Jufri Marmaya, Najihah Hanisah Omar, Maizatul Hasyima Basir, Rusliza Somchit, Muhammad Nazrul |
| author_facet | Zakaria, Zainul Amiruddin Roosli, Rushduddin Al Jufri Marmaya, Najihah Hanisah Omar, Maizatul Hasyima Basir, Rusliza Somchit, Muhammad Nazrul |
| author_sort | Zakaria, Zainul Amiruddin |
| collection | UPM |
| description | Dicranopteris linearis leaf has been reported to exert antinociceptive activity. The present study elucidates the possible mechanisms of antinociception modulated by the methanol extract of D. linearis leaves (MEDL) using various mouse models. The extract (25, 150, and 300 mg/kg) was administered orally to mice for 30 min priot to subjection to the acetic acid-induced writhing-, hot plate- or formalin-test to establish the antinociceptive profile of MEDL. The most effective dose was then used in the elucidation of possible mechanisms of action stage. The extract was also subjected to the phytochemical analyses. The results confirmed that MEDL exerted significant (p < 0.05) antinociceptive activity in those pain models as well as the capsaicin-, glutamate-, bradykinin- and phorbol 12-myristate 13-acetate (PMA)-induced paw licking model. Pretreatment with naloxone (a non-selective opioid antagonist) significantly (p < 0.05) reversed MEDL effect on thermal nociception. Only l-arginine (a nitric oxide (NO) donor) but not N(ω)-nitro-l-arginine methyl ester (l-NAME; a NO inhibitor) or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; a specific soluble guanylyl cyclase inhibitor) significantly (p < 0.05) modified MEDL effect on the writhing test. Several polyphenolics and volatile antinociceptive compounds were detected in MEDL. In conclusion, MEDL exerted the opioid/NO-mediated antinociceptive activity, thus, justify D. linearis as a potential source for new analgesic agents development. |
| first_indexed | 2024-03-06T08:40:28Z |
| format | Article |
| id | upm.eprints-38160 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:28Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-381602020-05-03T23:00:51Z http://psasir.upm.edu.my/id/eprint/38160/ Methanol extract of Dicranopteris linearis leaves attenuate pain via the modulation of opioid/NO-mediated pathway Zakaria, Zainul Amiruddin Roosli, Rushduddin Al Jufri Marmaya, Najihah Hanisah Omar, Maizatul Hasyima Basir, Rusliza Somchit, Muhammad Nazrul Dicranopteris linearis leaf has been reported to exert antinociceptive activity. The present study elucidates the possible mechanisms of antinociception modulated by the methanol extract of D. linearis leaves (MEDL) using various mouse models. The extract (25, 150, and 300 mg/kg) was administered orally to mice for 30 min priot to subjection to the acetic acid-induced writhing-, hot plate- or formalin-test to establish the antinociceptive profile of MEDL. The most effective dose was then used in the elucidation of possible mechanisms of action stage. The extract was also subjected to the phytochemical analyses. The results confirmed that MEDL exerted significant (p < 0.05) antinociceptive activity in those pain models as well as the capsaicin-, glutamate-, bradykinin- and phorbol 12-myristate 13-acetate (PMA)-induced paw licking model. Pretreatment with naloxone (a non-selective opioid antagonist) significantly (p < 0.05) reversed MEDL effect on thermal nociception. Only l-arginine (a nitric oxide (NO) donor) but not N(ω)-nitro-l-arginine methyl ester (l-NAME; a NO inhibitor) or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; a specific soluble guanylyl cyclase inhibitor) significantly (p < 0.05) modified MEDL effect on the writhing test. Several polyphenolics and volatile antinociceptive compounds were detected in MEDL. In conclusion, MEDL exerted the opioid/NO-mediated antinociceptive activity, thus, justify D. linearis as a potential source for new analgesic agents development. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38160/1/38160.pdf Zakaria, Zainul Amiruddin and Roosli, Rushduddin Al Jufri and Marmaya, Najihah Hanisah and Omar, Maizatul Hasyima and Basir, Rusliza and Somchit, Muhammad Nazrul (2020) Methanol extract of Dicranopteris linearis leaves attenuate pain via the modulation of opioid/NO-mediated pathway. Biomolecules, 10 (2). art. no. 280. pp. 1-23. ISSN 2218-273X https://www.mdpi.com/2218-273X/10/2/280 10.3390/biom10020280 |
| spellingShingle | Zakaria, Zainul Amiruddin Roosli, Rushduddin Al Jufri Marmaya, Najihah Hanisah Omar, Maizatul Hasyima Basir, Rusliza Somchit, Muhammad Nazrul Methanol extract of Dicranopteris linearis leaves attenuate pain via the modulation of opioid/NO-mediated pathway |
| title | Methanol extract of Dicranopteris linearis leaves attenuate pain via the modulation of opioid/NO-mediated pathway |
| title_full | Methanol extract of Dicranopteris linearis leaves attenuate pain via the modulation of opioid/NO-mediated pathway |
| title_fullStr | Methanol extract of Dicranopteris linearis leaves attenuate pain via the modulation of opioid/NO-mediated pathway |
| title_full_unstemmed | Methanol extract of Dicranopteris linearis leaves attenuate pain via the modulation of opioid/NO-mediated pathway |
| title_short | Methanol extract of Dicranopteris linearis leaves attenuate pain via the modulation of opioid/NO-mediated pathway |
| title_sort | methanol extract of dicranopteris linearis leaves attenuate pain via the modulation of opioid no mediated pathway |
| url | http://psasir.upm.edu.my/id/eprint/38160/1/38160.pdf |
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