Rapid evaluation and optimization of medium components governing tryptophan production by Pediococcus acidilactici TP-6 isolated from Malaysian food via statistical approaches
Tryptophan is one of the most extensively used amino acids in livestock industry owing to its effectiveness in enhancing the growth performance of animals. Conventionally, the production of tryptophan relies heavily on genetically modified Escherichia coli but its pathogenicity is a great concern. O...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38166/1/38166.pdf |
| _version_ | 1825949172876967936 |
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| author | Lim, Ye Heng Foo, Hooi Ling Loh, Teck Chwen Mohamad, Rosfarizan Abdul Rahim, Raha |
| author_facet | Lim, Ye Heng Foo, Hooi Ling Loh, Teck Chwen Mohamad, Rosfarizan Abdul Rahim, Raha |
| author_sort | Lim, Ye Heng |
| collection | UPM |
| description | Tryptophan is one of the most extensively used amino acids in livestock industry owing to its effectiveness in enhancing the growth performance of animals. Conventionally, the production of tryptophan relies heavily on genetically modified Escherichia coli but its pathogenicity is a great concern. Our recent study demonstrated that a lactic acid bacterium (LAB), Pediococcus acidilactici TP-6 that isolated from Malaysian food was a promising tryptophan producer. However, the tryptophan production must enhance further for viable industrial application. Hence, the current study evaluated the effects of medium components and optimized the medium composition for tryptophan production by P. acidilactici TP-6 statistically using Plackett-Burman Design, and Central Composite Design. The optimized medium containing molasses (14.06 g/L), meat extract (23.68 g/L), urea (5.56 g/L) and FeSO4 (0.024 g/L) significantly enhanced the tryptophan production by 150% as compared to the control de Man, Rogosa and Sharpe medium. The findings obtained in this study revealed that rapid evaluation and effective optimization of medium composition governing tryptophan production by P. acidilactici TP-6 were feasible via statistical approaches. Additionally, the current findings reveal the potential of utilizing LAB as a safer alternative tryptophan producer and provides insight for future exploitation of various amino acid productions by LAB. |
| first_indexed | 2024-03-06T08:40:29Z |
| format | Article |
| id | upm.eprints-38166 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:29Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-381662020-05-03T23:01:54Z http://psasir.upm.edu.my/id/eprint/38166/ Rapid evaluation and optimization of medium components governing tryptophan production by Pediococcus acidilactici TP-6 isolated from Malaysian food via statistical approaches Lim, Ye Heng Foo, Hooi Ling Loh, Teck Chwen Mohamad, Rosfarizan Abdul Rahim, Raha Tryptophan is one of the most extensively used amino acids in livestock industry owing to its effectiveness in enhancing the growth performance of animals. Conventionally, the production of tryptophan relies heavily on genetically modified Escherichia coli but its pathogenicity is a great concern. Our recent study demonstrated that a lactic acid bacterium (LAB), Pediococcus acidilactici TP-6 that isolated from Malaysian food was a promising tryptophan producer. However, the tryptophan production must enhance further for viable industrial application. Hence, the current study evaluated the effects of medium components and optimized the medium composition for tryptophan production by P. acidilactici TP-6 statistically using Plackett-Burman Design, and Central Composite Design. The optimized medium containing molasses (14.06 g/L), meat extract (23.68 g/L), urea (5.56 g/L) and FeSO4 (0.024 g/L) significantly enhanced the tryptophan production by 150% as compared to the control de Man, Rogosa and Sharpe medium. The findings obtained in this study revealed that rapid evaluation and effective optimization of medium composition governing tryptophan production by P. acidilactici TP-6 were feasible via statistical approaches. Additionally, the current findings reveal the potential of utilizing LAB as a safer alternative tryptophan producer and provides insight for future exploitation of various amino acid productions by LAB. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38166/1/38166.pdf Lim, Ye Heng and Foo, Hooi Ling and Loh, Teck Chwen and Mohamad, Rosfarizan and Abdul Rahim, Raha (2020) Rapid evaluation and optimization of medium components governing tryptophan production by Pediococcus acidilactici TP-6 isolated from Malaysian food via statistical approaches. Molecules, 25 (4). art. no. 779. pp. 1-27. ISSN 1420-3049 https://www.mdpi.com/1420-3049/25/4/779 10.3390/molecules25040779 |
| spellingShingle | Lim, Ye Heng Foo, Hooi Ling Loh, Teck Chwen Mohamad, Rosfarizan Abdul Rahim, Raha Rapid evaluation and optimization of medium components governing tryptophan production by Pediococcus acidilactici TP-6 isolated from Malaysian food via statistical approaches |
| title | Rapid evaluation and optimization of medium components governing tryptophan production by Pediococcus acidilactici TP-6 isolated from Malaysian food via statistical approaches |
| title_full | Rapid evaluation and optimization of medium components governing tryptophan production by Pediococcus acidilactici TP-6 isolated from Malaysian food via statistical approaches |
| title_fullStr | Rapid evaluation and optimization of medium components governing tryptophan production by Pediococcus acidilactici TP-6 isolated from Malaysian food via statistical approaches |
| title_full_unstemmed | Rapid evaluation and optimization of medium components governing tryptophan production by Pediococcus acidilactici TP-6 isolated from Malaysian food via statistical approaches |
| title_short | Rapid evaluation and optimization of medium components governing tryptophan production by Pediococcus acidilactici TP-6 isolated from Malaysian food via statistical approaches |
| title_sort | rapid evaluation and optimization of medium components governing tryptophan production by pediococcus acidilactici tp 6 isolated from malaysian food via statistical approaches |
| url | http://psasir.upm.edu.my/id/eprint/38166/1/38166.pdf |
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