Naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle
This study was conducted to examine the effects of different levels of lovastatin on the histological and sarcoplasmic proteome profile of goat skeletal muscle. A total of 20 intact male Saanen goats were randomly assigned in equal numbers to four groups and fed a total mixed ration containing 50% r...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38194/1/38194.pdf |
| _version_ | 1825949178505723904 |
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| author | Leo, Teik Kee Garba, Sani Abubakar, Danmaigoro Sazili, Awis Qurni Su, Candyrine Chui Len Jahromi, Mohammad Faseleh Goh, Yong Meng Ronimus, Ron Muetzel, Stefan Liang, Juan Boo |
| author_facet | Leo, Teik Kee Garba, Sani Abubakar, Danmaigoro Sazili, Awis Qurni Su, Candyrine Chui Len Jahromi, Mohammad Faseleh Goh, Yong Meng Ronimus, Ron Muetzel, Stefan Liang, Juan Boo |
| author_sort | Leo, Teik Kee |
| collection | UPM |
| description | This study was conducted to examine the effects of different levels of lovastatin on the histological and sarcoplasmic proteome profile of goat skeletal muscle. A total of 20 intact male Saanen goats were randomly assigned in equal numbers to four groups and fed a total mixed ration containing 50% rice straw, 22.8% concentrates and 27.2% of various proportions of untreated or treated palm kernel cake (PKC) to achieve the target daily intake levels of 0 (Control), 2 (Low), 4 (Medium) or 6 (High) mg lovastatin/kg BW. A histological examination discovered that the longissimus thoracis et lumborum muscle of animals from the Medium and High treatment groups showed abnormalities in terms of necrosis, degeneration, interstitial space and vacuolization. Our preliminary label-free proteomics analysis demonstrates that lovastatin supplementation induced complex modifications to the protein expression patterns of the skeletal muscle of the goat which were associated with the metabolism of carbohydrate and creatine, cell growth and development processes and other metabolic processes. The changes in these biochemical processes indicate perturbations in energy metabolism, which could play a major role in the development of myopathy. In conclusion, the present study suggests that supplementation of naturally produced lovastatin above 4 mg/kg BW could adversely affecting the health and wellbeing of treated animals. |
| first_indexed | 2024-03-06T08:40:34Z |
| format | Article |
| id | upm.eprints-38194 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:34Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-381942020-05-04T15:51:26Z http://psasir.upm.edu.my/id/eprint/38194/ Naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle Leo, Teik Kee Garba, Sani Abubakar, Danmaigoro Sazili, Awis Qurni Su, Candyrine Chui Len Jahromi, Mohammad Faseleh Goh, Yong Meng Ronimus, Ron Muetzel, Stefan Liang, Juan Boo This study was conducted to examine the effects of different levels of lovastatin on the histological and sarcoplasmic proteome profile of goat skeletal muscle. A total of 20 intact male Saanen goats were randomly assigned in equal numbers to four groups and fed a total mixed ration containing 50% rice straw, 22.8% concentrates and 27.2% of various proportions of untreated or treated palm kernel cake (PKC) to achieve the target daily intake levels of 0 (Control), 2 (Low), 4 (Medium) or 6 (High) mg lovastatin/kg BW. A histological examination discovered that the longissimus thoracis et lumborum muscle of animals from the Medium and High treatment groups showed abnormalities in terms of necrosis, degeneration, interstitial space and vacuolization. Our preliminary label-free proteomics analysis demonstrates that lovastatin supplementation induced complex modifications to the protein expression patterns of the skeletal muscle of the goat which were associated with the metabolism of carbohydrate and creatine, cell growth and development processes and other metabolic processes. The changes in these biochemical processes indicate perturbations in energy metabolism, which could play a major role in the development of myopathy. In conclusion, the present study suggests that supplementation of naturally produced lovastatin above 4 mg/kg BW could adversely affecting the health and wellbeing of treated animals. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38194/1/38194.pdf Leo, Teik Kee and Garba, Sani and Abubakar, Danmaigoro and Sazili, Awis Qurni and Su, Candyrine Chui Len and Jahromi, Mohammad Faseleh and Goh, Yong Meng and Ronimus, Ron and Muetzel, Stefan and Liang, Juan Boo (2020) Naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle. Animals, 10 (1). art. no. 72. pp. 1-19. ISSN 2076-2615 https://www.mdpi.com/2076-2615/10/1/72 10.3390/ani10010072 |
| spellingShingle | Leo, Teik Kee Garba, Sani Abubakar, Danmaigoro Sazili, Awis Qurni Su, Candyrine Chui Len Jahromi, Mohammad Faseleh Goh, Yong Meng Ronimus, Ron Muetzel, Stefan Liang, Juan Boo Naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle |
| title | Naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle |
| title_full | Naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle |
| title_fullStr | Naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle |
| title_full_unstemmed | Naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle |
| title_short | Naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle |
| title_sort | naturally produced lovastatin modifies the histology and proteome profile of goat skeletal muscle |
| url | http://psasir.upm.edu.my/id/eprint/38194/1/38194.pdf |
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