Comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from Malaysian foods
Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, β-glucosidase, β-m...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2019
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/38239/1/38239.pdf |
| _version_ | 1796973306309509120 |
|---|---|
| author | Lee, Fu Haw Wan, Suet Ying Foo, Hooi Ling Loh, Teck Chwen Mohamad, Rosfarizan Abdul Rahim, Raha Idrus, Zulkifli |
| author_facet | Lee, Fu Haw Wan, Suet Ying Foo, Hooi Ling Loh, Teck Chwen Mohamad, Rosfarizan Abdul Rahim, Raha Idrus, Zulkifli |
| author_sort | Lee, Fu Haw |
| collection | UPM |
| description | Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, β-glucosidase, β-mannosidase, and β-mannanase activities. Therefore, extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities of seven Lactobacillus plantarum strains (a prominent species of LAB) isolated from Malaysian foods were compared in this study. The biotransformation of palm kernel cake (PKC) biomass mediated by selected L. plantarum strains was subsequently conducted. The results obtained in this study exhibited the studied L. plantarum strains produced versatile multi extracellular hydrolytic enzyme activities that were active from acidic to alkaline pH conditions. The highest total score of extracellular hydrolytic enzyme activities were recorded by L. plantarum RI11, L. plantarum RG11, and L. plantarum RG14. Therefore, they were selected for the subsequent biotransformation of PKC biomass via SSF. The hydrolytic enzyme activities of treated PKC extract were compared for each sampling interval. The scanning electron microscopy analyses revealed the formation of extracellular matrices around L. plantarum strains attached to the surface of PKC biomass during SSF, inferring that the investigated L. plantarum strains have the capability to grow on PKC biomass and perform synergistic secretions of various extracellular proteolytic, cellulolytic, and hemicellulolytic enzymes that were essential for the effective biodegradation of PKC. The substantial growth of selected L. plamtraum strains on PKC during SSF revealed the promising application of selected L. plantarum strains as a biotransformation agent for cellulosic biomass. |
| first_indexed | 2024-03-06T08:40:42Z |
| format | Article |
| id | upm.eprints-38239 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:42Z |
| publishDate | 2019 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-382392020-05-04T16:05:13Z http://psasir.upm.edu.my/id/eprint/38239/ Comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from Malaysian foods Lee, Fu Haw Wan, Suet Ying Foo, Hooi Ling Loh, Teck Chwen Mohamad, Rosfarizan Abdul Rahim, Raha Idrus, Zulkifli Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, β-glucosidase, β-mannosidase, and β-mannanase activities. Therefore, extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities of seven Lactobacillus plantarum strains (a prominent species of LAB) isolated from Malaysian foods were compared in this study. The biotransformation of palm kernel cake (PKC) biomass mediated by selected L. plantarum strains was subsequently conducted. The results obtained in this study exhibited the studied L. plantarum strains produced versatile multi extracellular hydrolytic enzyme activities that were active from acidic to alkaline pH conditions. The highest total score of extracellular hydrolytic enzyme activities were recorded by L. plantarum RI11, L. plantarum RG11, and L. plantarum RG14. Therefore, they were selected for the subsequent biotransformation of PKC biomass via SSF. The hydrolytic enzyme activities of treated PKC extract were compared for each sampling interval. The scanning electron microscopy analyses revealed the formation of extracellular matrices around L. plantarum strains attached to the surface of PKC biomass during SSF, inferring that the investigated L. plantarum strains have the capability to grow on PKC biomass and perform synergistic secretions of various extracellular proteolytic, cellulolytic, and hemicellulolytic enzymes that were essential for the effective biodegradation of PKC. The substantial growth of selected L. plamtraum strains on PKC during SSF revealed the promising application of selected L. plantarum strains as a biotransformation agent for cellulosic biomass. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38239/1/38239.pdf Lee, Fu Haw and Wan, Suet Ying and Foo, Hooi Ling and Loh, Teck Chwen and Mohamad, Rosfarizan and Abdul Rahim, Raha and Idrus, Zulkifli (2019) Comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from Malaysian foods. International Journal of Molecular Sciences, 20 (20). art. no. 4979. pp. 1-26. ISSN 1661-6596; ESSN: 1422-0067 https://www.mdpi.com/1422-0067/20/20/4979 10.3390/ijms20204979 |
| spellingShingle | Lee, Fu Haw Wan, Suet Ying Foo, Hooi Ling Loh, Teck Chwen Mohamad, Rosfarizan Abdul Rahim, Raha Idrus, Zulkifli Comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from Malaysian foods |
| title | Comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from Malaysian foods |
| title_full | Comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from Malaysian foods |
| title_fullStr | Comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from Malaysian foods |
| title_full_unstemmed | Comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from Malaysian foods |
| title_short | Comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from Malaysian foods |
| title_sort | comparative study of extracellular proteolytic cellulolytic and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from malaysian foods |
| url | http://psasir.upm.edu.my/id/eprint/38239/1/38239.pdf |
| work_keys_str_mv | AT leefuhaw comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods AT wansuetying comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods AT foohooiling comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods AT lohteckchwen comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods AT mohamadrosfarizan comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods AT abdulrahimraha comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods AT idruszulkifli comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods |