Microbial phosphotriesterase: structure, function, and biotechnological applications
The role of phosphotriesterase as an enzyme which is able to hydrolyze organophosphate compounds cannot be disputed. Contamination by organophosphate (OP) compounds in the environment is alarming, and even more worrying is the toxicity of this compound, which affects the nervous system. Thus, it is...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38289/1/38289.pdf |
| _version_ | 1796973316630642688 |
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| author | Latip, Wahhida Knight, Victor Feizal Abdul Halim, Norhana Ong, Keat Khim Mohd Kasim, Noor Azilah Wan Yunus, Wan Md. Zin Mohd Noor, Siti Aminah Mohamad Ali, Mohd Shukuri |
| author_facet | Latip, Wahhida Knight, Victor Feizal Abdul Halim, Norhana Ong, Keat Khim Mohd Kasim, Noor Azilah Wan Yunus, Wan Md. Zin Mohd Noor, Siti Aminah Mohamad Ali, Mohd Shukuri |
| author_sort | Latip, Wahhida |
| collection | UPM |
| description | The role of phosphotriesterase as an enzyme which is able to hydrolyze organophosphate compounds cannot be disputed. Contamination by organophosphate (OP) compounds in the environment is alarming, and even more worrying is the toxicity of this compound, which affects the nervous system. Thus, it is important to find a safer way to detoxify, detect and recuperate from the toxicity effects of this compound. Phosphotriesterases (PTEs) are mostly isolated from soil bacteria and are classified as metalloenzymes or metal-dependent enzymes that contain bimetals at the active site. There are three separate pockets to accommodate the substrate into the active site of each PTE. This enzyme generally shows a high catalytic activity towards phosphotriesters. These microbial enzymes are robust and easy to manipulate. Currently, PTEs are widely studied for the detection, detoxification, and enzyme therapies for OP compound poisoning incidents. The discovery and understanding of PTEs would pave ways for greener approaches in biotechnological applications and to solve environmental issues relating to OP contamination. |
| first_indexed | 2024-03-06T08:40:51Z |
| format | Article |
| id | upm.eprints-38289 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:51Z |
| publishDate | 2019 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-382892020-05-04T16:12:22Z http://psasir.upm.edu.my/id/eprint/38289/ Microbial phosphotriesterase: structure, function, and biotechnological applications Latip, Wahhida Knight, Victor Feizal Abdul Halim, Norhana Ong, Keat Khim Mohd Kasim, Noor Azilah Wan Yunus, Wan Md. Zin Mohd Noor, Siti Aminah Mohamad Ali, Mohd Shukuri The role of phosphotriesterase as an enzyme which is able to hydrolyze organophosphate compounds cannot be disputed. Contamination by organophosphate (OP) compounds in the environment is alarming, and even more worrying is the toxicity of this compound, which affects the nervous system. Thus, it is important to find a safer way to detoxify, detect and recuperate from the toxicity effects of this compound. Phosphotriesterases (PTEs) are mostly isolated from soil bacteria and are classified as metalloenzymes or metal-dependent enzymes that contain bimetals at the active site. There are three separate pockets to accommodate the substrate into the active site of each PTE. This enzyme generally shows a high catalytic activity towards phosphotriesters. These microbial enzymes are robust and easy to manipulate. Currently, PTEs are widely studied for the detection, detoxification, and enzyme therapies for OP compound poisoning incidents. The discovery and understanding of PTEs would pave ways for greener approaches in biotechnological applications and to solve environmental issues relating to OP contamination. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38289/1/38289.pdf Latip, Wahhida and Knight, Victor Feizal and Abdul Halim, Norhana and Ong, Keat Khim and Mohd Kasim, Noor Azilah and Wan Yunus, Wan Md. Zin and Mohd Noor, Siti Aminah and Mohamad Ali, Mohd Shukuri (2019) Microbial phosphotriesterase: structure, function, and biotechnological applications. Catalysts, 9 (8). art. no. 671. pp. 1-11. ISSN 2073-4344 https://www.mdpi.com/2073-4344/9/8/671 10.3390/catal9080671 |
| spellingShingle | Latip, Wahhida Knight, Victor Feizal Abdul Halim, Norhana Ong, Keat Khim Mohd Kasim, Noor Azilah Wan Yunus, Wan Md. Zin Mohd Noor, Siti Aminah Mohamad Ali, Mohd Shukuri Microbial phosphotriesterase: structure, function, and biotechnological applications |
| title | Microbial phosphotriesterase: structure, function, and biotechnological applications |
| title_full | Microbial phosphotriesterase: structure, function, and biotechnological applications |
| title_fullStr | Microbial phosphotriesterase: structure, function, and biotechnological applications |
| title_full_unstemmed | Microbial phosphotriesterase: structure, function, and biotechnological applications |
| title_short | Microbial phosphotriesterase: structure, function, and biotechnological applications |
| title_sort | microbial phosphotriesterase structure function and biotechnological applications |
| url | http://psasir.upm.edu.my/id/eprint/38289/1/38289.pdf |
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