Preparation and characterization of self nano-emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro
Citral is an active compound naturally found in lemongrass, lemon, and lime. Although this pale-yellow liquid confers low water solubility, the compound has been reported to possess good therapeutic features including antiproliferative and anticancer modalities. The self nano-emulsifying drug delive...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2019
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/38299/1/38299.pdf |
| _version_ | 1825949198958198784 |
|---|---|
| author | Mohd Izham, Mira Nadiah Hussin, Yazmin Aziz, Muhammad Nazirul Mubin Yeap, Swee Keong Rahman, Heshu Sulaiman Masarudin, Mas Jaffri Mohamad, Nurul Elyani Abdullah, Rasedee Mohammed Alitheen, Noorjahan Banu |
| author_facet | Mohd Izham, Mira Nadiah Hussin, Yazmin Aziz, Muhammad Nazirul Mubin Yeap, Swee Keong Rahman, Heshu Sulaiman Masarudin, Mas Jaffri Mohamad, Nurul Elyani Abdullah, Rasedee Mohammed Alitheen, Noorjahan Banu |
| author_sort | Mohd Izham, Mira Nadiah |
| collection | UPM |
| description | Citral is an active compound naturally found in lemongrass, lemon, and lime. Although this pale-yellow liquid confers low water solubility, the compound has been reported to possess good therapeutic features including antiproliferative and anticancer modalities. The self nano-emulsifying drug delivery system (SNEDDS) is a type of liquid-lipid nanocarrier that is suitable for the loading of insolubilized oil-based compound such as Citral. This study reports the design and optimization of a SNEDDS formulation, synthesis and characterization as well as loading with Citral (CIT-SNEDDS). Further assessment of theantiproliferative effects of CIT-SNEDDS towards colorectal cancer cells was also conducted. SNEDDS composed of coconut oil, dimethyl sulfoxide (DMSO) and Tween 80. CIT-SNEDDS was prepared via gentle agitation of SNEDDS with 0.5% Citral for 72 h at room temperature. Physicochemical characterization was performed using several physicochemical analyses. The average particle size of CIT-SNEDDS was16.86 ± 0.15 nm, zeta potential of 0.58 ± 0.19 mV, and polydispersity index (PDI) of 0.23 ± 0.01. In vitro drug release of Citral from CIT-SNEDDS was 79.25% of release, and for Citral the release percentage was 93.56% over 72 h. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done to determine the cytotoxicity effect of CIT-SNEDDS in human colorectal cancer cell lines HT29 and SW620. The half maximal inhibitory concentrations (IC50) for 72 hof CIT-SNEDDS and Citral on SW620 were 16.50 ± 0.87 µg/mL and 22.50 ± 2.50 µg/mL, respectively. The IC50 values of CIT-SNEDDS and Citral after 72 h of treatment on HT29 were 34.10 ± 0.30 µg/mL and 21.77 ± 0.23 µg/mL, respectively. This study strongly suggests that CIT-SNEDDS has permitted the sustained release of Citral and that CIT-SNEDDS constitutes a potential soluble drug nanocarrier that is effective against colorectal cancer cells. |
| first_indexed | 2024-03-06T08:40:53Z |
| format | Article |
| id | upm.eprints-38299 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:53Z |
| publishDate | 2019 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-382992020-05-04T16:13:28Z http://psasir.upm.edu.my/id/eprint/38299/ Preparation and characterization of self nano-emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro Mohd Izham, Mira Nadiah Hussin, Yazmin Aziz, Muhammad Nazirul Mubin Yeap, Swee Keong Rahman, Heshu Sulaiman Masarudin, Mas Jaffri Mohamad, Nurul Elyani Abdullah, Rasedee Mohammed Alitheen, Noorjahan Banu Citral is an active compound naturally found in lemongrass, lemon, and lime. Although this pale-yellow liquid confers low water solubility, the compound has been reported to possess good therapeutic features including antiproliferative and anticancer modalities. The self nano-emulsifying drug delivery system (SNEDDS) is a type of liquid-lipid nanocarrier that is suitable for the loading of insolubilized oil-based compound such as Citral. This study reports the design and optimization of a SNEDDS formulation, synthesis and characterization as well as loading with Citral (CIT-SNEDDS). Further assessment of theantiproliferative effects of CIT-SNEDDS towards colorectal cancer cells was also conducted. SNEDDS composed of coconut oil, dimethyl sulfoxide (DMSO) and Tween 80. CIT-SNEDDS was prepared via gentle agitation of SNEDDS with 0.5% Citral for 72 h at room temperature. Physicochemical characterization was performed using several physicochemical analyses. The average particle size of CIT-SNEDDS was16.86 ± 0.15 nm, zeta potential of 0.58 ± 0.19 mV, and polydispersity index (PDI) of 0.23 ± 0.01. In vitro drug release of Citral from CIT-SNEDDS was 79.25% of release, and for Citral the release percentage was 93.56% over 72 h. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done to determine the cytotoxicity effect of CIT-SNEDDS in human colorectal cancer cell lines HT29 and SW620. The half maximal inhibitory concentrations (IC50) for 72 hof CIT-SNEDDS and Citral on SW620 were 16.50 ± 0.87 µg/mL and 22.50 ± 2.50 µg/mL, respectively. The IC50 values of CIT-SNEDDS and Citral after 72 h of treatment on HT29 were 34.10 ± 0.30 µg/mL and 21.77 ± 0.23 µg/mL, respectively. This study strongly suggests that CIT-SNEDDS has permitted the sustained release of Citral and that CIT-SNEDDS constitutes a potential soluble drug nanocarrier that is effective against colorectal cancer cells. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38299/1/38299.pdf Mohd Izham, Mira Nadiah and Hussin, Yazmin and Aziz, Muhammad Nazirul Mubin and Yeap, Swee Keong and Rahman, Heshu Sulaiman and Masarudin, Mas Jaffri and Mohamad, Nurul Elyani and Abdullah, Rasedee and Mohammed Alitheen, Noorjahan Banu (2019) Preparation and characterization of self nano-emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro. Nanomaterials, 9 (7). art. no. 1028. pp. 1-18. ISSN 2079-4991 https://www.mdpi.com/2079-4991/9/7/1028 10.3390/nano9071028 |
| spellingShingle | Mohd Izham, Mira Nadiah Hussin, Yazmin Aziz, Muhammad Nazirul Mubin Yeap, Swee Keong Rahman, Heshu Sulaiman Masarudin, Mas Jaffri Mohamad, Nurul Elyani Abdullah, Rasedee Mohammed Alitheen, Noorjahan Banu Preparation and characterization of self nano-emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro |
| title | Preparation and characterization of self nano-emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro |
| title_full | Preparation and characterization of self nano-emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro |
| title_fullStr | Preparation and characterization of self nano-emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro |
| title_full_unstemmed | Preparation and characterization of self nano-emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro |
| title_short | Preparation and characterization of self nano-emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro |
| title_sort | preparation and characterization of self nano emulsifying drug delivery system loaded with citraland its antiproliferative effect on colorectal cells in vitro |
| url | http://psasir.upm.edu.my/id/eprint/38299/1/38299.pdf |
| work_keys_str_mv | AT mohdizhammiranadiah preparationandcharacterizationofselfnanoemulsifyingdrugdeliverysystemloadedwithcitralanditsantiproliferativeeffectoncolorectalcellsinvitro AT hussinyazmin preparationandcharacterizationofselfnanoemulsifyingdrugdeliverysystemloadedwithcitralanditsantiproliferativeeffectoncolorectalcellsinvitro AT azizmuhammadnazirulmubin preparationandcharacterizationofselfnanoemulsifyingdrugdeliverysystemloadedwithcitralanditsantiproliferativeeffectoncolorectalcellsinvitro AT yeapsweekeong preparationandcharacterizationofselfnanoemulsifyingdrugdeliverysystemloadedwithcitralanditsantiproliferativeeffectoncolorectalcellsinvitro AT rahmanheshusulaiman preparationandcharacterizationofselfnanoemulsifyingdrugdeliverysystemloadedwithcitralanditsantiproliferativeeffectoncolorectalcellsinvitro AT masarudinmasjaffri preparationandcharacterizationofselfnanoemulsifyingdrugdeliverysystemloadedwithcitralanditsantiproliferativeeffectoncolorectalcellsinvitro AT mohamadnurulelyani preparationandcharacterizationofselfnanoemulsifyingdrugdeliverysystemloadedwithcitralanditsantiproliferativeeffectoncolorectalcellsinvitro AT abdullahrasedee preparationandcharacterizationofselfnanoemulsifyingdrugdeliverysystemloadedwithcitralanditsantiproliferativeeffectoncolorectalcellsinvitro AT mohammedalitheennoorjahanbanu preparationandcharacterizationofselfnanoemulsifyingdrugdeliverysystemloadedwithcitralanditsantiproliferativeeffectoncolorectalcellsinvitro |