Ampelopsin E reduces the invasiveness of the triple negative breast cancer cell line, MDA-MB-231
Breast cancer is the most common and the second leading cause of cancer-related deaths in women. It has two distinctive hallmarks: rapid abnormal growth and the ability to invade and metastasize. During metastasis, cancer cells are thought to form actin-rich protrusions, called invadopodia, which de...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38300/1/38300.pdf |
| _version_ | 1796973318833700864 |
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| author | Tieng, Francis Yew Fu Saiful Yazan, Latifah Md Hashim, Nur Fariesha Khaza'ai, Huzwah Ahmat, Norizan Gopalsamy, Banulata Wibowo, Agustono |
| author_facet | Tieng, Francis Yew Fu Saiful Yazan, Latifah Md Hashim, Nur Fariesha Khaza'ai, Huzwah Ahmat, Norizan Gopalsamy, Banulata Wibowo, Agustono |
| author_sort | Tieng, Francis Yew Fu |
| collection | UPM |
| description | Breast cancer is the most common and the second leading cause of cancer-related deaths in women. It has two distinctive hallmarks: rapid abnormal growth and the ability to invade and metastasize. During metastasis, cancer cells are thought to form actin-rich protrusions, called invadopodia, which degrade the extracellular matrix. Current breast cancer treatments, particularly chemotherapy, comes with adverse effects like immunosuppression, resistance development and secondary tumour formation. Hence, naturally-occurring molecules claimed to be less toxic are being studied as new drug candidates. Ampelopsin E, a natural oligostilbene extracted from Dryobalanops species, has exhibited various pharmacological properties, including anticancer and anti-inflammatory activities. However, there is yet no scientific evidence of the effects of ampelopsin E towards metastasis. Scratch assay, transwell migration and invasion assays, invadopodia and gelatin degradation assays, and ELISA were used to determine the effects of ampelopsin E towards the invasiveness of MDA-MB-231 cells. Strikingly in this study, ampelopsin E was able to halt migration, transmigration and invasion in MDA-MB-231 cells by reducing formation of invadopodia and its degradation capability through significant reduction (p < 0.05) in expression levels of PDGF, MMP2, MMP9 and MMP14. In conclusion, ampelopsin E reduced the invasiveness of MDA-MB-231 cells and was proven to be a potential alternative in treating TNBC. |
| first_indexed | 2024-03-06T08:40:53Z |
| format | Article |
| id | upm.eprints-38300 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:40:53Z |
| publishDate | 2019 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-383002020-05-04T16:14:23Z http://psasir.upm.edu.my/id/eprint/38300/ Ampelopsin E reduces the invasiveness of the triple negative breast cancer cell line, MDA-MB-231 Tieng, Francis Yew Fu Saiful Yazan, Latifah Md Hashim, Nur Fariesha Khaza'ai, Huzwah Ahmat, Norizan Gopalsamy, Banulata Wibowo, Agustono Breast cancer is the most common and the second leading cause of cancer-related deaths in women. It has two distinctive hallmarks: rapid abnormal growth and the ability to invade and metastasize. During metastasis, cancer cells are thought to form actin-rich protrusions, called invadopodia, which degrade the extracellular matrix. Current breast cancer treatments, particularly chemotherapy, comes with adverse effects like immunosuppression, resistance development and secondary tumour formation. Hence, naturally-occurring molecules claimed to be less toxic are being studied as new drug candidates. Ampelopsin E, a natural oligostilbene extracted from Dryobalanops species, has exhibited various pharmacological properties, including anticancer and anti-inflammatory activities. However, there is yet no scientific evidence of the effects of ampelopsin E towards metastasis. Scratch assay, transwell migration and invasion assays, invadopodia and gelatin degradation assays, and ELISA were used to determine the effects of ampelopsin E towards the invasiveness of MDA-MB-231 cells. Strikingly in this study, ampelopsin E was able to halt migration, transmigration and invasion in MDA-MB-231 cells by reducing formation of invadopodia and its degradation capability through significant reduction (p < 0.05) in expression levels of PDGF, MMP2, MMP9 and MMP14. In conclusion, ampelopsin E reduced the invasiveness of MDA-MB-231 cells and was proven to be a potential alternative in treating TNBC. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38300/1/38300.pdf Tieng, Francis Yew Fu and Saiful Yazan, Latifah and Md Hashim, Nur Fariesha and Khaza'ai, Huzwah and Ahmat, Norizan and Gopalsamy, Banulata and Wibowo, Agustono (2019) Ampelopsin E reduces the invasiveness of the triple negative breast cancer cell line, MDA-MB-231. Molecules, 24 (14). art. no. 2619. pp. 1-25. ISSN 1420-3049 https://www.mdpi.com/1420-3049/24/14/2619 10.3390/molecules24142619 |
| spellingShingle | Tieng, Francis Yew Fu Saiful Yazan, Latifah Md Hashim, Nur Fariesha Khaza'ai, Huzwah Ahmat, Norizan Gopalsamy, Banulata Wibowo, Agustono Ampelopsin E reduces the invasiveness of the triple negative breast cancer cell line, MDA-MB-231 |
| title | Ampelopsin E reduces the invasiveness of the triple negative breast cancer cell line, MDA-MB-231 |
| title_full | Ampelopsin E reduces the invasiveness of the triple negative breast cancer cell line, MDA-MB-231 |
| title_fullStr | Ampelopsin E reduces the invasiveness of the triple negative breast cancer cell line, MDA-MB-231 |
| title_full_unstemmed | Ampelopsin E reduces the invasiveness of the triple negative breast cancer cell line, MDA-MB-231 |
| title_short | Ampelopsin E reduces the invasiveness of the triple negative breast cancer cell line, MDA-MB-231 |
| title_sort | ampelopsin e reduces the invasiveness of the triple negative breast cancer cell line mda mb 231 |
| url | http://psasir.upm.edu.my/id/eprint/38300/1/38300.pdf |
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