Polymer pen lithography-fabricated DNA arrays for highly sensitive and selective detection of unamplified Ganoderma boninense DNA
There is an increasing demand for lithography methods to enable the fabrication of diagnostic devices for the biomedical and agri-food sectors. In this regard, scanning probe lithography methods have emerged as a possible approach for this purpose, as they are not only convenient, robust and accessi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2019
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/38415/1/38415.pdf |
| _version_ | 1796973342242111488 |
|---|---|
| author | Rani, Ekta Mohshim, Siti Akhtar Ahmad, Muhammad Zamharir Goodacre, Royston Alang Ahmad, Shahrul Ainliah Wong, Lu Shin |
| author_facet | Rani, Ekta Mohshim, Siti Akhtar Ahmad, Muhammad Zamharir Goodacre, Royston Alang Ahmad, Shahrul Ainliah Wong, Lu Shin |
| author_sort | Rani, Ekta |
| collection | UPM |
| description | There is an increasing demand for lithography methods to enable the fabrication of diagnostic devices for the biomedical and agri-food sectors. In this regard, scanning probe lithography methods have emerged as a possible approach for this purpose, as they are not only convenient, robust and accessible, but also enable the deposition of “soft” materials such as complex organic molecules and biomolecules. In this report, the use of polymer pen lithography for the fabrication of DNA oligonucleotide arrays is described, together with the application of the arrays for the sensitive and selective detection of Ganoderma boninense, a fungal pathogen of the oil palm. When used in a sandwich assay format with DNA-conjugated gold nanoparticles, this system is able to generate a visually observable result in the presence of the target DNA. This assay is able to detect as little as 30 ng of Ganoderma-derived DNA without any pre-amplification and without the need for specialist laboratory equipment or training. |
| first_indexed | 2024-03-06T08:41:14Z |
| format | Article |
| id | upm.eprints-38415 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:41:14Z |
| publishDate | 2019 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-384152020-05-04T16:47:41Z http://psasir.upm.edu.my/id/eprint/38415/ Polymer pen lithography-fabricated DNA arrays for highly sensitive and selective detection of unamplified Ganoderma boninense DNA Rani, Ekta Mohshim, Siti Akhtar Ahmad, Muhammad Zamharir Goodacre, Royston Alang Ahmad, Shahrul Ainliah Wong, Lu Shin There is an increasing demand for lithography methods to enable the fabrication of diagnostic devices for the biomedical and agri-food sectors. In this regard, scanning probe lithography methods have emerged as a possible approach for this purpose, as they are not only convenient, robust and accessible, but also enable the deposition of “soft” materials such as complex organic molecules and biomolecules. In this report, the use of polymer pen lithography for the fabrication of DNA oligonucleotide arrays is described, together with the application of the arrays for the sensitive and selective detection of Ganoderma boninense, a fungal pathogen of the oil palm. When used in a sandwich assay format with DNA-conjugated gold nanoparticles, this system is able to generate a visually observable result in the presence of the target DNA. This assay is able to detect as little as 30 ng of Ganoderma-derived DNA without any pre-amplification and without the need for specialist laboratory equipment or training. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38415/1/38415.pdf Rani, Ekta and Mohshim, Siti Akhtar and Ahmad, Muhammad Zamharir and Goodacre, Royston and Alang Ahmad, Shahrul Ainliah and Wong, Lu Shin (2019) Polymer pen lithography-fabricated DNA arrays for highly sensitive and selective detection of unamplified Ganoderma boninense DNA. Polymers, 11 (3). art. no. 561. pp. 1-12. ISSN 2073-4360 https://www.mdpi.com/2073-4360/11/3/561 10.3390/polym11030561 |
| spellingShingle | Rani, Ekta Mohshim, Siti Akhtar Ahmad, Muhammad Zamharir Goodacre, Royston Alang Ahmad, Shahrul Ainliah Wong, Lu Shin Polymer pen lithography-fabricated DNA arrays for highly sensitive and selective detection of unamplified Ganoderma boninense DNA |
| title | Polymer pen lithography-fabricated DNA arrays for highly sensitive and selective detection of unamplified Ganoderma boninense DNA |
| title_full | Polymer pen lithography-fabricated DNA arrays for highly sensitive and selective detection of unamplified Ganoderma boninense DNA |
| title_fullStr | Polymer pen lithography-fabricated DNA arrays for highly sensitive and selective detection of unamplified Ganoderma boninense DNA |
| title_full_unstemmed | Polymer pen lithography-fabricated DNA arrays for highly sensitive and selective detection of unamplified Ganoderma boninense DNA |
| title_short | Polymer pen lithography-fabricated DNA arrays for highly sensitive and selective detection of unamplified Ganoderma boninense DNA |
| title_sort | polymer pen lithography fabricated dna arrays for highly sensitive and selective detection of unamplified ganoderma boninense dna |
| url | http://psasir.upm.edu.my/id/eprint/38415/1/38415.pdf |
| work_keys_str_mv | AT raniekta polymerpenlithographyfabricateddnaarraysforhighlysensitiveandselectivedetectionofunamplifiedganodermaboninensedna AT mohshimsitiakhtar polymerpenlithographyfabricateddnaarraysforhighlysensitiveandselectivedetectionofunamplifiedganodermaboninensedna AT ahmadmuhammadzamharir polymerpenlithographyfabricateddnaarraysforhighlysensitiveandselectivedetectionofunamplifiedganodermaboninensedna AT goodacreroyston polymerpenlithographyfabricateddnaarraysforhighlysensitiveandselectivedetectionofunamplifiedganodermaboninensedna AT alangahmadshahrulainliah polymerpenlithographyfabricateddnaarraysforhighlysensitiveandselectivedetectionofunamplifiedganodermaboninensedna AT wonglushin polymerpenlithographyfabricateddnaarraysforhighlysensitiveandselectivedetectionofunamplifiedganodermaboninensedna |