Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures
Mesophyll cells of asparagus will undergo dedifferentiation when placed in culture medium. Cells may be mechanically isolated from asparagus cladodes rapidly and in sufficient quantities to enable a study of this switch in development at the microscopic level. During dedifferentiation a reproducible...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1989
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38564/1/DEDIFFERENTIATION%20OF%20ASPARAGUS%20OFFICINALIS%20L.%20MESOPHYLL%20CELLS.pdf |
| _version_ | 1825949248298942464 |
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| author | Paul, E. Harikrishna, K. Fioroni, O. Draper, J. |
| author_facet | Paul, E. Harikrishna, K. Fioroni, O. Draper, J. |
| author_sort | Paul, E. |
| collection | UPM |
| description | Mesophyll cells of asparagus will undergo dedifferentiation when placed in culture medium. Cells may be mechanically isolated from asparagus cladodes rapidly and in sufficient quantities to enable a study of this switch in development at the microscopic level. During dedifferentiation a reproducible, ordered sequence of events occurs that includes revocery from wound stress, reactivation of the cell cycle followed by cell expansion and cell division. Dedifferentiation in vitro is a complicated process which, at the cellular level, appears to resemble most aspects of dedifferentation during callus formation at wound sites in intact plants and large explants. Results from this intial study have been used to define developmental phases of dedifferentiation, which will be used in future molecular analyses of this system. |
| first_indexed | 2024-03-06T08:41:38Z |
| format | Article |
| id | upm.eprints-38564 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:41:38Z |
| publishDate | 1989 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-385642016-01-11T06:57:49Z http://psasir.upm.edu.my/id/eprint/38564/ Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures Paul, E. Harikrishna, K. Fioroni, O. Draper, J. Mesophyll cells of asparagus will undergo dedifferentiation when placed in culture medium. Cells may be mechanically isolated from asparagus cladodes rapidly and in sufficient quantities to enable a study of this switch in development at the microscopic level. During dedifferentiation a reproducible, ordered sequence of events occurs that includes revocery from wound stress, reactivation of the cell cycle followed by cell expansion and cell division. Dedifferentiation in vitro is a complicated process which, at the cellular level, appears to resemble most aspects of dedifferentation during callus formation at wound sites in intact plants and large explants. Results from this intial study have been used to define developmental phases of dedifferentiation, which will be used in future molecular analyses of this system. Elsevier 1989 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/38564/1/DEDIFFERENTIATION%20OF%20ASPARAGUS%20OFFICINALIS%20L.%20MESOPHYLL%20CELLS.pdf Paul, E. and Harikrishna, K. and Fioroni, O. and Draper, J. (1989) Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures. Plant Science, 65 (1). pp. 111-117. ISSN 0168-9452; ESSN: 1873-2259 http://www.sciencedirect.com/science/article/pii/0168945289902136 10.1016/0168-9452(89)90213-6 |
| spellingShingle | Paul, E. Harikrishna, K. Fioroni, O. Draper, J. Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures |
| title | Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures |
| title_full | Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures |
| title_fullStr | Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures |
| title_full_unstemmed | Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures |
| title_short | Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures |
| title_sort | dedifferentiation of asparagus officinalis l mesophyll cells during initiation of cell cultures |
| url | http://psasir.upm.edu.my/id/eprint/38564/1/DEDIFFERENTIATION%20OF%20ASPARAGUS%20OFFICINALIS%20L.%20MESOPHYLL%20CELLS.pdf |
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