Chloroplast dedifferentiation in mechanically isolated asparagus cells during culture initiation
Mechanically isolated asparagus (Asparagus officinalis) mesophyll cells dedifferentiate and divide when cultured in the dark in a medium containing sucrose. A strong correlation was observed between the onset of cell division and a loss of photosynthetic capacity. For the first 8 to 9 d of culture,...
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| Format: | Article |
| Language: | English |
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American Society of Plant Biologists
1992
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| Online Access: | http://psasir.upm.edu.my/id/eprint/39692/1/6.%20Chloroplast%20Dedifferentiation%20in%20Mechanically%20Isolated%20Asparagus%20Cells%20during%20Culture%20Initiation%C2%B9.pdf |
| _version_ | 1796973588987772928 |
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| author | Harikrishna, Kulaveerasingham Darby, Robert Draper, John |
| author_facet | Harikrishna, Kulaveerasingham Darby, Robert Draper, John |
| author_sort | Harikrishna, Kulaveerasingham |
| collection | UPM |
| description | Mechanically isolated asparagus (Asparagus officinalis) mesophyll cells dedifferentiate and divide when cultured in the dark in a medium containing sucrose. A strong correlation was observed between the onset of cell division and a loss of photosynthetic capacity. For the first 8 to 9 d of culture, there was no change in chloroplast size or morphology. However, following this period, the chloroplasts divided to form smaller proplastid-like structures. The gross chlorophyll content of the cell population did not change, suggesting that the loss of photosynthetic potential was not by senescence. Northern analysis showed that mRNA of the small subunit of ribulose l,5-bisphosphate carboxylase/OJeygenase was undetectable within 1 d postisolation, which was quicker than in
dark-treated plants. The mRNA of the large subunit of ribulose 1,S-bisphosphate carboxylase/oxygenase decreased to low levels within 2 d of cell isolation. Both the large and small subunits of ribulose l,S-bisphosphate carboxylase/oxygenase protein showed a gradual reduction in abundance, falling to basal levels by days 6 to 7, which coincided with the onset of rapid cell division. A similar
trend was observed with chloroplast rRNA molecules, which decreased to basal levels by day 6 in culture
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| first_indexed | 2024-03-06T08:44:52Z |
| format | Article |
| id | upm.eprints-39692 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:44:52Z |
| publishDate | 1992 |
| publisher | American Society of Plant Biologists |
| record_format | dspace |
| spelling | upm.eprints-396922015-08-12T07:09:49Z http://psasir.upm.edu.my/id/eprint/39692/ Chloroplast dedifferentiation in mechanically isolated asparagus cells during culture initiation Harikrishna, Kulaveerasingham Darby, Robert Draper, John Mechanically isolated asparagus (Asparagus officinalis) mesophyll cells dedifferentiate and divide when cultured in the dark in a medium containing sucrose. A strong correlation was observed between the onset of cell division and a loss of photosynthetic capacity. For the first 8 to 9 d of culture, there was no change in chloroplast size or morphology. However, following this period, the chloroplasts divided to form smaller proplastid-like structures. The gross chlorophyll content of the cell population did not change, suggesting that the loss of photosynthetic potential was not by senescence. Northern analysis showed that mRNA of the small subunit of ribulose l,5-bisphosphate carboxylase/OJeygenase was undetectable within 1 d postisolation, which was quicker than in dark-treated plants. The mRNA of the large subunit of ribulose 1,S-bisphosphate carboxylase/oxygenase decreased to low levels within 2 d of cell isolation. Both the large and small subunits of ribulose l,S-bisphosphate carboxylase/oxygenase protein showed a gradual reduction in abundance, falling to basal levels by days 6 to 7, which coincided with the onset of rapid cell division. A similar trend was observed with chloroplast rRNA molecules, which decreased to basal levels by day 6 in culture American Society of Plant Biologists 1992 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/39692/1/6.%20Chloroplast%20Dedifferentiation%20in%20Mechanically%20Isolated%20Asparagus%20Cells%20during%20Culture%20Initiation%C2%B9.pdf Harikrishna, Kulaveerasingham and Darby, Robert and Draper, John (1992) Chloroplast dedifferentiation in mechanically isolated asparagus cells during culture initiation. Plant Physiology, 100. pp. 1177-1183. ISSN 0032-0889 |
| spellingShingle | Harikrishna, Kulaveerasingham Darby, Robert Draper, John Chloroplast dedifferentiation in mechanically isolated asparagus cells during culture initiation |
| title | Chloroplast dedifferentiation in mechanically isolated
asparagus cells during culture initiation
|
| title_full | Chloroplast dedifferentiation in mechanically isolated
asparagus cells during culture initiation
|
| title_fullStr | Chloroplast dedifferentiation in mechanically isolated
asparagus cells during culture initiation
|
| title_full_unstemmed | Chloroplast dedifferentiation in mechanically isolated
asparagus cells during culture initiation
|
| title_short | Chloroplast dedifferentiation in mechanically isolated
asparagus cells during culture initiation
|
| title_sort | chloroplast dedifferentiation in mechanically isolated asparagus cells during culture initiation |
| url | http://psasir.upm.edu.my/id/eprint/39692/1/6.%20Chloroplast%20Dedifferentiation%20in%20Mechanically%20Isolated%20Asparagus%20Cells%20during%20Culture%20Initiation%C2%B9.pdf |
| work_keys_str_mv | AT harikrishnakulaveerasingham chloroplastdedifferentiationinmechanicallyisolatedasparaguscellsduringcultureinitiation AT darbyrobert chloroplastdedifferentiationinmechanicallyisolatedasparaguscellsduringcultureinitiation AT draperjohn chloroplastdedifferentiationinmechanicallyisolatedasparaguscellsduringcultureinitiation |