Carboxymethyl-cellulase and filter-paperase activities of napier grass fermentation by new bacterial isolates
Cellulosic biomass is a renewable and most abundant resource with high potential for bioconversion to biofuels. It can be degraded by cellulase produced by cellulolytic producing bacteria. Proximately, three different types of cellulase needed for effective enzymatic hydrolysis; endoglucanase (CMCas...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/39919/1/39919.pdf |
| _version_ | 1825949501614981120 |
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| author | Manap, Shahirah Wan Omar, Wan Siti Atikah Saad, Wan Zuhainis Harith, Siti Suhaila Mohd Noor, Badrul Hisham Mohd Mydin, Marlina Kamarullah, Shahida Hanum Mohamad, Rosfarizan |
| author_facet | Manap, Shahirah Wan Omar, Wan Siti Atikah Saad, Wan Zuhainis Harith, Siti Suhaila Mohd Noor, Badrul Hisham Mohd Mydin, Marlina Kamarullah, Shahida Hanum Mohamad, Rosfarizan |
| author_sort | Manap, Shahirah |
| collection | UPM |
| description | Cellulosic biomass is a renewable and most abundant resource with high potential for bioconversion to biofuels. It can be degraded by cellulase produced by cellulolytic producing bacteria. Proximately, three different types of cellulase needed for effective enzymatic hydrolysis; endoglucanase (CMCase), exocellobiohydrolase and β -glucosidase. Total cellulase activity can be determined by measuring reducing sugar derived from filter paper (FPase) while endoglucanase (CMCase) can be determined by measure reducing sugar release from carboxymethyl- cellulose (CMC). In this study, the CMCase and FPase activities of new six isolates from different environments (compost: C5/1 and C5/2; ponds: L5/1, L6/2, and L6/5; and decayed wood: D6/1) were examined according methods recommended by the International Union of Pure and Applied Chemistry (IUPAC).The fermentation has been conducted for 4 days incubation period at 37°C and agitated at 150 rpm. Napier grass has been used as a substrate. As a result, the C5/2 exhibited the highest enzymes activities of CMCase and FPase, which were 2.205U/mL and 0.39U/mL, respectively. |
| first_indexed | 2024-03-06T08:45:32Z |
| format | Conference or Workshop Item |
| id | upm.eprints-39919 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:45:32Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | upm.eprints-399192016-06-30T06:39:18Z http://psasir.upm.edu.my/id/eprint/39919/ Carboxymethyl-cellulase and filter-paperase activities of napier grass fermentation by new bacterial isolates Manap, Shahirah Wan Omar, Wan Siti Atikah Saad, Wan Zuhainis Harith, Siti Suhaila Mohd Noor, Badrul Hisham Mohd Mydin, Marlina Kamarullah, Shahida Hanum Mohamad, Rosfarizan Cellulosic biomass is a renewable and most abundant resource with high potential for bioconversion to biofuels. It can be degraded by cellulase produced by cellulolytic producing bacteria. Proximately, three different types of cellulase needed for effective enzymatic hydrolysis; endoglucanase (CMCase), exocellobiohydrolase and β -glucosidase. Total cellulase activity can be determined by measuring reducing sugar derived from filter paper (FPase) while endoglucanase (CMCase) can be determined by measure reducing sugar release from carboxymethyl- cellulose (CMC). In this study, the CMCase and FPase activities of new six isolates from different environments (compost: C5/1 and C5/2; ponds: L5/1, L6/2, and L6/5; and decayed wood: D6/1) were examined according methods recommended by the International Union of Pure and Applied Chemistry (IUPAC).The fermentation has been conducted for 4 days incubation period at 37°C and agitated at 150 rpm. Napier grass has been used as a substrate. As a result, the C5/2 exhibited the highest enzymes activities of CMCase and FPase, which were 2.205U/mL and 0.39U/mL, respectively. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/39919/1/39919.pdf Manap, Shahirah and Wan Omar, Wan Siti Atikah and Saad, Wan Zuhainis and Harith, Siti Suhaila and Mohd Noor, Badrul Hisham and Mohd Mydin, Marlina and Kamarullah, Shahida Hanum and Mohamad, Rosfarizan (2014) Carboxymethyl-cellulase and filter-paperase activities of napier grass fermentation by new bacterial isolates. In: 32nd Symposium of the Malaysian Society for Microbiology (MSM2014), 6-8 Dec. 2014, Terengganu Equestrian Resort (TER), Terengganu, Malaysia . (pp. 250-253). |
| spellingShingle | Manap, Shahirah Wan Omar, Wan Siti Atikah Saad, Wan Zuhainis Harith, Siti Suhaila Mohd Noor, Badrul Hisham Mohd Mydin, Marlina Kamarullah, Shahida Hanum Mohamad, Rosfarizan Carboxymethyl-cellulase and filter-paperase activities of napier grass fermentation by new bacterial isolates |
| title | Carboxymethyl-cellulase and filter-paperase activities of napier grass fermentation by new bacterial isolates |
| title_full | Carboxymethyl-cellulase and filter-paperase activities of napier grass fermentation by new bacterial isolates |
| title_fullStr | Carboxymethyl-cellulase and filter-paperase activities of napier grass fermentation by new bacterial isolates |
| title_full_unstemmed | Carboxymethyl-cellulase and filter-paperase activities of napier grass fermentation by new bacterial isolates |
| title_short | Carboxymethyl-cellulase and filter-paperase activities of napier grass fermentation by new bacterial isolates |
| title_sort | carboxymethyl cellulase and filter paperase activities of napier grass fermentation by new bacterial isolates |
| url | http://psasir.upm.edu.my/id/eprint/39919/1/39919.pdf |
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