Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species
The antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of seven Hedyotisspecies were investigated. The antioxidant activity was evaluated by the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods while the radical scavenging...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2005
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/40322/1/Antioxidant%2C%20radical-scavenging%2C%20anti-inflammatory%2C%20cytotoxic%20and%20antibacterial%20activities%20of%20methanolic%20extracts%20of%20some%20Hedyotis%20species.pdf |
| _version_ | 1796973717551579136 |
|---|---|
| author | Ahmad, Rohaya Ali, Abdul Manaf Ahmad Israf, Daud Ismail, Nor Hadiani Shaari, Khozirah Lajis, Nordin |
| author_facet | Ahmad, Rohaya Ali, Abdul Manaf Ahmad Israf, Daud Ismail, Nor Hadiani Shaari, Khozirah Lajis, Nordin |
| author_sort | Ahmad, Rohaya |
| collection | UPM |
| description | The antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of seven Hedyotisspecies were investigated. The antioxidant activity was evaluated by the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods while the radical scavenging activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The anti-inflammatory activity related to NO inhibition of the plant extracts was measured by the Griess assay while cytotoxicity were measured by the MTT assay against CEM-SS cell line. The antibacterial bioassay (against 4 bacteria, i.e. Bacillus subtilis B28 (mutant), Bacillus subtilis B29 (wild-type), Pseudomonas aeruginosa UI 60690 and methicillin resistant Staphylococcus aureus, (MRSA) was also carried out using the disc-diffusion method. All tested extracts exhibited very strong antioxidant properties when compared to Vitamin E (α-tocopherol) with percent inhibition of 89–98% in the FTC and 60–95% in the TBA assays. In the DPPH method, H. herbacea exhibited the strongest radical scavenging activity with an IC50 value of 32 μg/ml. The results from the Griess assay showed that the tested extracts are weak inhibitors of NO synthase. However, all tested extracts exhibited moderate cytotoxic properties against CEM-SS cell line giving CD50 values in the range of 21–41 μg/ml. In the antibacterial bioassay, the stems and the roots of H. capitellata showed moderate activity against the 4 tested bacteria while the leaves showed moderate activity towards B. subtilis B28, MRSA and P. aeruginosa only. The roots of H. dichotoma showed strong antibacterial activity against all 4 bacteria. All other extracts did not exhibit any antibacterial activity. |
| first_indexed | 2024-03-06T08:46:44Z |
| format | Article |
| id | upm.eprints-40322 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:46:44Z |
| publishDate | 2005 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-403222015-09-17T01:34:54Z http://psasir.upm.edu.my/id/eprint/40322/ Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species Ahmad, Rohaya Ali, Abdul Manaf Ahmad Israf, Daud Ismail, Nor Hadiani Shaari, Khozirah Lajis, Nordin The antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of seven Hedyotisspecies were investigated. The antioxidant activity was evaluated by the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods while the radical scavenging activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The anti-inflammatory activity related to NO inhibition of the plant extracts was measured by the Griess assay while cytotoxicity were measured by the MTT assay against CEM-SS cell line. The antibacterial bioassay (against 4 bacteria, i.e. Bacillus subtilis B28 (mutant), Bacillus subtilis B29 (wild-type), Pseudomonas aeruginosa UI 60690 and methicillin resistant Staphylococcus aureus, (MRSA) was also carried out using the disc-diffusion method. All tested extracts exhibited very strong antioxidant properties when compared to Vitamin E (α-tocopherol) with percent inhibition of 89–98% in the FTC and 60–95% in the TBA assays. In the DPPH method, H. herbacea exhibited the strongest radical scavenging activity with an IC50 value of 32 μg/ml. The results from the Griess assay showed that the tested extracts are weak inhibitors of NO synthase. However, all tested extracts exhibited moderate cytotoxic properties against CEM-SS cell line giving CD50 values in the range of 21–41 μg/ml. In the antibacterial bioassay, the stems and the roots of H. capitellata showed moderate activity against the 4 tested bacteria while the leaves showed moderate activity towards B. subtilis B28, MRSA and P. aeruginosa only. The roots of H. dichotoma showed strong antibacterial activity against all 4 bacteria. All other extracts did not exhibit any antibacterial activity. Elsevier 2005-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40322/1/Antioxidant%2C%20radical-scavenging%2C%20anti-inflammatory%2C%20cytotoxic%20and%20antibacterial%20activities%20of%20methanolic%20extracts%20of%20some%20Hedyotis%20species.pdf Ahmad, Rohaya and Ali, Abdul Manaf and Ahmad Israf, Daud and Ismail, Nor Hadiani and Shaari, Khozirah and Lajis, Nordin (2005) Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species. Life Sciences, 76 (17). pp. 1953-1964. ISSN 0024-3205; ESSN: 1879-0631 http://www.sciencedirect.com/science/article/pii/S0024320505000032 10.1016/j.lfs.2004.08.039 |
| spellingShingle | Ahmad, Rohaya Ali, Abdul Manaf Ahmad Israf, Daud Ismail, Nor Hadiani Shaari, Khozirah Lajis, Nordin Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species |
| title | Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species |
| title_full | Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species |
| title_fullStr | Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species |
| title_full_unstemmed | Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species |
| title_short | Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species |
| title_sort | antioxidant radical scavenging anti inflammatory cytotoxic and antibacterial activities of methanolic extracts of some hedyotis species |
| url | http://psasir.upm.edu.my/id/eprint/40322/1/Antioxidant%2C%20radical-scavenging%2C%20anti-inflammatory%2C%20cytotoxic%20and%20antibacterial%20activities%20of%20methanolic%20extracts%20of%20some%20Hedyotis%20species.pdf |
| work_keys_str_mv | AT ahmadrohaya antioxidantradicalscavengingantiinflammatorycytotoxicandantibacterialactivitiesofmethanolicextractsofsomehedyotisspecies AT aliabdulmanaf antioxidantradicalscavengingantiinflammatorycytotoxicandantibacterialactivitiesofmethanolicextractsofsomehedyotisspecies AT ahmadisrafdaud antioxidantradicalscavengingantiinflammatorycytotoxicandantibacterialactivitiesofmethanolicextractsofsomehedyotisspecies AT ismailnorhadiani antioxidantradicalscavengingantiinflammatorycytotoxicandantibacterialactivitiesofmethanolicextractsofsomehedyotisspecies AT shaarikhozirah antioxidantradicalscavengingantiinflammatorycytotoxicandantibacterialactivitiesofmethanolicextractsofsomehedyotisspecies AT lajisnordin antioxidantradicalscavengingantiinflammatorycytotoxicandantibacterialactivitiesofmethanolicextractsofsomehedyotisspecies |